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Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress

The Pacific white shrimp (Litopenaeus vannamei) is an important cultured crustacean species worldwide. However, little is known about the molecular mechanism of this species involved in the response to cold stress. In this study, four separate RNA-Seq libraries of L. vannamei were generated from 13°...

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Autores principales: Huang, Wen, Ren, Chunhua, Li, Hongmei, Huo, Da, Wang, Yanhong, Jiang, Xiao, Tian, Yushun, Luo, Peng, Chen, Ting, Hu, Chaoqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456072/
https://www.ncbi.nlm.nih.gov/pubmed/28575089
http://dx.doi.org/10.1371/journal.pone.0178604
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author Huang, Wen
Ren, Chunhua
Li, Hongmei
Huo, Da
Wang, Yanhong
Jiang, Xiao
Tian, Yushun
Luo, Peng
Chen, Ting
Hu, Chaoqun
author_facet Huang, Wen
Ren, Chunhua
Li, Hongmei
Huo, Da
Wang, Yanhong
Jiang, Xiao
Tian, Yushun
Luo, Peng
Chen, Ting
Hu, Chaoqun
author_sort Huang, Wen
collection PubMed
description The Pacific white shrimp (Litopenaeus vannamei) is an important cultured crustacean species worldwide. However, little is known about the molecular mechanism of this species involved in the response to cold stress. In this study, four separate RNA-Seq libraries of L. vannamei were generated from 13°C stress and control temperature. Total 29,662 of Unigenes and overall of 19,619 annotated genes were obtained. Three comparisons were carried out among the four libraries, in which 72 of the top 20% of differentially-expressed genes were obtained, 15 GO and 5 KEGG temperature-sensitive pathways were fished out. Catalytic activity (GO: 0003824) and Metabolic pathways (ko01100) were the most annotated GO and KEGG pathways in response to cold stress, respectively. In addition, Calcium, MAPK cascade, Transcription factor and Serine/threonine-protein kinase signal pathway were picked out and clustered. Serine/threonine-protein kinase signal pathway might play more important roles in cold adaptation, while other three signal pathway were not widely transcribed. Our results had summarized the differentially-expressed genes and suggested the major important signaling pathways and related genes. These findings provide the first profile insight into the molecular basis of L. vannamei response to cold stress.
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spelling pubmed-54560722017-06-12 Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress Huang, Wen Ren, Chunhua Li, Hongmei Huo, Da Wang, Yanhong Jiang, Xiao Tian, Yushun Luo, Peng Chen, Ting Hu, Chaoqun PLoS One Research Article The Pacific white shrimp (Litopenaeus vannamei) is an important cultured crustacean species worldwide. However, little is known about the molecular mechanism of this species involved in the response to cold stress. In this study, four separate RNA-Seq libraries of L. vannamei were generated from 13°C stress and control temperature. Total 29,662 of Unigenes and overall of 19,619 annotated genes were obtained. Three comparisons were carried out among the four libraries, in which 72 of the top 20% of differentially-expressed genes were obtained, 15 GO and 5 KEGG temperature-sensitive pathways were fished out. Catalytic activity (GO: 0003824) and Metabolic pathways (ko01100) were the most annotated GO and KEGG pathways in response to cold stress, respectively. In addition, Calcium, MAPK cascade, Transcription factor and Serine/threonine-protein kinase signal pathway were picked out and clustered. Serine/threonine-protein kinase signal pathway might play more important roles in cold adaptation, while other three signal pathway were not widely transcribed. Our results had summarized the differentially-expressed genes and suggested the major important signaling pathways and related genes. These findings provide the first profile insight into the molecular basis of L. vannamei response to cold stress. Public Library of Science 2017-06-02 /pmc/articles/PMC5456072/ /pubmed/28575089 http://dx.doi.org/10.1371/journal.pone.0178604 Text en © 2017 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Huang, Wen
Ren, Chunhua
Li, Hongmei
Huo, Da
Wang, Yanhong
Jiang, Xiao
Tian, Yushun
Luo, Peng
Chen, Ting
Hu, Chaoqun
Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress
title Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress
title_full Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress
title_fullStr Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress
title_full_unstemmed Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress
title_short Transcriptomic analyses on muscle tissues of Litopenaeus vannamei provide the first profile insight into the response to low temperature stress
title_sort transcriptomic analyses on muscle tissues of litopenaeus vannamei provide the first profile insight into the response to low temperature stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456072/
https://www.ncbi.nlm.nih.gov/pubmed/28575089
http://dx.doi.org/10.1371/journal.pone.0178604
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