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Synthesis of CdSe Quantum Dots Using Fusarium oxysporum
CdSe quantum dots are often used in industry as fluorescent materials. In this study, CdSe quantum dots were synthesized using Fusarium oxysporum. The cadmium and selenium concentration, pH, and temperature for the culture of F. oxysporum (Fusarium oxysporum) were optimized for the synthesis, and th...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456586/ https://www.ncbi.nlm.nih.gov/pubmed/28773975 http://dx.doi.org/10.3390/ma9100855 |
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author | Yamaguchi, Takaaki Tsuruda, Yoshijiro Furukawa, Tomohiro Negishi, Lumi Imura, Yuki Sakuda, Shohei Yoshimura, Etsuro Suzuki, Michio |
author_facet | Yamaguchi, Takaaki Tsuruda, Yoshijiro Furukawa, Tomohiro Negishi, Lumi Imura, Yuki Sakuda, Shohei Yoshimura, Etsuro Suzuki, Michio |
author_sort | Yamaguchi, Takaaki |
collection | PubMed |
description | CdSe quantum dots are often used in industry as fluorescent materials. In this study, CdSe quantum dots were synthesized using Fusarium oxysporum. The cadmium and selenium concentration, pH, and temperature for the culture of F. oxysporum (Fusarium oxysporum) were optimized for the synthesis, and the CdSe quantum dots obtained from the mycelial cells of F. oxysporum were observed by transmission electron microscopy. Ultra-thin sections of F. oxysporum showed that the CdSe quantum dots were precipitated in the intracellular space, indicating that cadmium and selenium ions were incorporated into the cell and that the quantum dots were synthesized with intracellular metabolites. To reveal differences in F. oxysporum metabolism, cell extracts of F. oxysporum, before and after CdSe synthesis, were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results suggested that the amount of superoxide dismutase (SOD) decreased after CdSe synthesis. Fluorescence microscopy revealed that cytoplasmic superoxide increased significantly after CdSe synthesis. The accumulation of superoxide may increase the expression of various metabolites that play a role in reducing Se(4+) to Se(2−) and inhibit the aggregation of CdSe to make nanoparticles. |
format | Online Article Text |
id | pubmed-5456586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54565862017-07-28 Synthesis of CdSe Quantum Dots Using Fusarium oxysporum Yamaguchi, Takaaki Tsuruda, Yoshijiro Furukawa, Tomohiro Negishi, Lumi Imura, Yuki Sakuda, Shohei Yoshimura, Etsuro Suzuki, Michio Materials (Basel) Article CdSe quantum dots are often used in industry as fluorescent materials. In this study, CdSe quantum dots were synthesized using Fusarium oxysporum. The cadmium and selenium concentration, pH, and temperature for the culture of F. oxysporum (Fusarium oxysporum) were optimized for the synthesis, and the CdSe quantum dots obtained from the mycelial cells of F. oxysporum were observed by transmission electron microscopy. Ultra-thin sections of F. oxysporum showed that the CdSe quantum dots were precipitated in the intracellular space, indicating that cadmium and selenium ions were incorporated into the cell and that the quantum dots were synthesized with intracellular metabolites. To reveal differences in F. oxysporum metabolism, cell extracts of F. oxysporum, before and after CdSe synthesis, were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results suggested that the amount of superoxide dismutase (SOD) decreased after CdSe synthesis. Fluorescence microscopy revealed that cytoplasmic superoxide increased significantly after CdSe synthesis. The accumulation of superoxide may increase the expression of various metabolites that play a role in reducing Se(4+) to Se(2−) and inhibit the aggregation of CdSe to make nanoparticles. MDPI 2016-10-20 /pmc/articles/PMC5456586/ /pubmed/28773975 http://dx.doi.org/10.3390/ma9100855 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yamaguchi, Takaaki Tsuruda, Yoshijiro Furukawa, Tomohiro Negishi, Lumi Imura, Yuki Sakuda, Shohei Yoshimura, Etsuro Suzuki, Michio Synthesis of CdSe Quantum Dots Using Fusarium oxysporum |
title | Synthesis of CdSe Quantum Dots Using Fusarium oxysporum |
title_full | Synthesis of CdSe Quantum Dots Using Fusarium oxysporum |
title_fullStr | Synthesis of CdSe Quantum Dots Using Fusarium oxysporum |
title_full_unstemmed | Synthesis of CdSe Quantum Dots Using Fusarium oxysporum |
title_short | Synthesis of CdSe Quantum Dots Using Fusarium oxysporum |
title_sort | synthesis of cdse quantum dots using fusarium oxysporum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456586/ https://www.ncbi.nlm.nih.gov/pubmed/28773975 http://dx.doi.org/10.3390/ma9100855 |
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