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Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications

Gelatin methacryloyl (GelMA) has been increasingly considered as an important bioink material due to its tailorable mechanical properties, good biocompatibility, and ability to be photopolymerized in situ as well as printability. GelMA can be classified into two types: type A GelMA (a product from a...

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Autores principales: Lee, Bae Hoon, Lum, Nathaniel, Seow, Li Yuan, Lim, Pei Qi, Tan, Lay Poh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456596/
https://www.ncbi.nlm.nih.gov/pubmed/28773918
http://dx.doi.org/10.3390/ma9100797
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author Lee, Bae Hoon
Lum, Nathaniel
Seow, Li Yuan
Lim, Pei Qi
Tan, Lay Poh
author_facet Lee, Bae Hoon
Lum, Nathaniel
Seow, Li Yuan
Lim, Pei Qi
Tan, Lay Poh
author_sort Lee, Bae Hoon
collection PubMed
description Gelatin methacryloyl (GelMA) has been increasingly considered as an important bioink material due to its tailorable mechanical properties, good biocompatibility, and ability to be photopolymerized in situ as well as printability. GelMA can be classified into two types: type A GelMA (a product from acid treatment) and type B GelMA (a product from alkali treatment). In current literature, there is little research on the comparison of type A GelMA and type B GelMA in terms of synthesis, rheological properties, and printability for bioink applications. Here, we report the synthesis, rheological properties, and printability of types A and B GelMA. Types A and B GelMA samples with different degrees of substitution (DS) were prepared in a controllable manner by a time-lapse loading method of methacrylic anhydride (MAA) and different feed ratios of MAA to gelatin. Type B GelMA tended to have a slightly higher DS compared to type A GelMA, especially in a lower feed ratio of MAA to gelatin. All the type A and type B GelMA solutions with different DS exhibited shear thinning behaviours at 37 °C. However, only GelMA with a high DS had an easy-to-extrude feature at room temperature. The cell-laden printed constructs of types A and B GelMA at 20% w/v showed around 75% cell viability.
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spelling pubmed-54565962017-07-28 Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications Lee, Bae Hoon Lum, Nathaniel Seow, Li Yuan Lim, Pei Qi Tan, Lay Poh Materials (Basel) Article Gelatin methacryloyl (GelMA) has been increasingly considered as an important bioink material due to its tailorable mechanical properties, good biocompatibility, and ability to be photopolymerized in situ as well as printability. GelMA can be classified into two types: type A GelMA (a product from acid treatment) and type B GelMA (a product from alkali treatment). In current literature, there is little research on the comparison of type A GelMA and type B GelMA in terms of synthesis, rheological properties, and printability for bioink applications. Here, we report the synthesis, rheological properties, and printability of types A and B GelMA. Types A and B GelMA samples with different degrees of substitution (DS) were prepared in a controllable manner by a time-lapse loading method of methacrylic anhydride (MAA) and different feed ratios of MAA to gelatin. Type B GelMA tended to have a slightly higher DS compared to type A GelMA, especially in a lower feed ratio of MAA to gelatin. All the type A and type B GelMA solutions with different DS exhibited shear thinning behaviours at 37 °C. However, only GelMA with a high DS had an easy-to-extrude feature at room temperature. The cell-laden printed constructs of types A and B GelMA at 20% w/v showed around 75% cell viability. MDPI 2016-09-24 /pmc/articles/PMC5456596/ /pubmed/28773918 http://dx.doi.org/10.3390/ma9100797 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Bae Hoon
Lum, Nathaniel
Seow, Li Yuan
Lim, Pei Qi
Tan, Lay Poh
Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications
title Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications
title_full Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications
title_fullStr Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications
title_full_unstemmed Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications
title_short Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications
title_sort synthesis and characterization of types a and b gelatin methacryloyl for bioink applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456596/
https://www.ncbi.nlm.nih.gov/pubmed/28773918
http://dx.doi.org/10.3390/ma9100797
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