Cargando…

Preparation and Evaluation of Dexamethasone-Loaded Electrospun Nanofiber Sheets as a Sustained Drug Delivery System

Recently, electrospinning technology has been widely used as a processing method to make nanofiber sheets (NS) for biomedical applications because of its unique features, such as ease of fabrication and high surface area. To develop a sustained dexamethasone (Dex) delivery system, in this work, poly...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Jin Woo, Lee, Hye Yun, Park, Seung Hun, Park, Ji Hoon, Kim, Jae Ho, Min, Byoung Hyun, Kim, Moon Suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456713/
https://www.ncbi.nlm.nih.gov/pubmed/28773300
http://dx.doi.org/10.3390/ma9030175
_version_ 1783241354716905472
author Lee, Jin Woo
Lee, Hye Yun
Park, Seung Hun
Park, Ji Hoon
Kim, Jae Ho
Min, Byoung Hyun
Kim, Moon Suk
author_facet Lee, Jin Woo
Lee, Hye Yun
Park, Seung Hun
Park, Ji Hoon
Kim, Jae Ho
Min, Byoung Hyun
Kim, Moon Suk
author_sort Lee, Jin Woo
collection PubMed
description Recently, electrospinning technology has been widely used as a processing method to make nanofiber sheets (NS) for biomedical applications because of its unique features, such as ease of fabrication and high surface area. To develop a sustained dexamethasone (Dex) delivery system, in this work, poly(ε-caprolactone-co-l-lactide) (PCLA) copolymer with controllable biodegradability was synthesized and further utilized to prepare electrospun Dex-loaded NS using water-insoluble Dex (Dex(b)) or water-soluble Dex (Dex(s)). The Dex-NS obtained by electrospinning exhibited randomly oriented and interconnected fibrillar structures. The in vitro and in vivo degradation of Dex-NS was confirmed over a period of a few weeks by gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR). The evaluation of in vitro and in vivo Dex(b) and Dex(s) release from Dex-NS showed an initial burst of Dex(b) at day 1 and, thereafter, almost the same amount of release as Dex(b) for up to 28 days. In contrast, Dex(s)-NS exhibited a small initial burst of Dex(s) and a first-order releasing profile from Dex-NS. In conclusion, Dex-NS exhibited sustained in vitro and in vivo Dex(s) release for a prolonged period, as well as controlled biodegradation of the NS over a defined treatment period.
format Online
Article
Text
id pubmed-5456713
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-54567132017-07-28 Preparation and Evaluation of Dexamethasone-Loaded Electrospun Nanofiber Sheets as a Sustained Drug Delivery System Lee, Jin Woo Lee, Hye Yun Park, Seung Hun Park, Ji Hoon Kim, Jae Ho Min, Byoung Hyun Kim, Moon Suk Materials (Basel) Article Recently, electrospinning technology has been widely used as a processing method to make nanofiber sheets (NS) for biomedical applications because of its unique features, such as ease of fabrication and high surface area. To develop a sustained dexamethasone (Dex) delivery system, in this work, poly(ε-caprolactone-co-l-lactide) (PCLA) copolymer with controllable biodegradability was synthesized and further utilized to prepare electrospun Dex-loaded NS using water-insoluble Dex (Dex(b)) or water-soluble Dex (Dex(s)). The Dex-NS obtained by electrospinning exhibited randomly oriented and interconnected fibrillar structures. The in vitro and in vivo degradation of Dex-NS was confirmed over a period of a few weeks by gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR). The evaluation of in vitro and in vivo Dex(b) and Dex(s) release from Dex-NS showed an initial burst of Dex(b) at day 1 and, thereafter, almost the same amount of release as Dex(b) for up to 28 days. In contrast, Dex(s)-NS exhibited a small initial burst of Dex(s) and a first-order releasing profile from Dex-NS. In conclusion, Dex-NS exhibited sustained in vitro and in vivo Dex(s) release for a prolonged period, as well as controlled biodegradation of the NS over a defined treatment period. MDPI 2016-03-08 /pmc/articles/PMC5456713/ /pubmed/28773300 http://dx.doi.org/10.3390/ma9030175 Text en © 2016 by the authors; Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Jin Woo
Lee, Hye Yun
Park, Seung Hun
Park, Ji Hoon
Kim, Jae Ho
Min, Byoung Hyun
Kim, Moon Suk
Preparation and Evaluation of Dexamethasone-Loaded Electrospun Nanofiber Sheets as a Sustained Drug Delivery System
title Preparation and Evaluation of Dexamethasone-Loaded Electrospun Nanofiber Sheets as a Sustained Drug Delivery System
title_full Preparation and Evaluation of Dexamethasone-Loaded Electrospun Nanofiber Sheets as a Sustained Drug Delivery System
title_fullStr Preparation and Evaluation of Dexamethasone-Loaded Electrospun Nanofiber Sheets as a Sustained Drug Delivery System
title_full_unstemmed Preparation and Evaluation of Dexamethasone-Loaded Electrospun Nanofiber Sheets as a Sustained Drug Delivery System
title_short Preparation and Evaluation of Dexamethasone-Loaded Electrospun Nanofiber Sheets as a Sustained Drug Delivery System
title_sort preparation and evaluation of dexamethasone-loaded electrospun nanofiber sheets as a sustained drug delivery system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456713/
https://www.ncbi.nlm.nih.gov/pubmed/28773300
http://dx.doi.org/10.3390/ma9030175
work_keys_str_mv AT leejinwoo preparationandevaluationofdexamethasoneloadedelectrospunnanofibersheetsasasustaineddrugdeliverysystem
AT leehyeyun preparationandevaluationofdexamethasoneloadedelectrospunnanofibersheetsasasustaineddrugdeliverysystem
AT parkseunghun preparationandevaluationofdexamethasoneloadedelectrospunnanofibersheetsasasustaineddrugdeliverysystem
AT parkjihoon preparationandevaluationofdexamethasoneloadedelectrospunnanofibersheetsasasustaineddrugdeliverysystem
AT kimjaeho preparationandevaluationofdexamethasoneloadedelectrospunnanofibersheetsasasustaineddrugdeliverysystem
AT minbyounghyun preparationandevaluationofdexamethasoneloadedelectrospunnanofibersheetsasasustaineddrugdeliverysystem
AT kimmoonsuk preparationandevaluationofdexamethasoneloadedelectrospunnanofibersheetsasasustaineddrugdeliverysystem