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Preparation of Nanofibrous Structure of Mesoporous Bioactive Glass Microbeads for Biomedical Applications

A highly ordered, mesoporous (pore size 2~50 nm) bioactive glass (MBG) structure has a greater surface area and pore volume and excellent bone-forming bioactivity compared with traditional bioactive glasses (BGs). Hence, MBGs have been used in drug delivery and bone tissue engineering. MBGs can be d...

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Autores principales: Tsai, Shiao-Wen, Chang, Yu-Han, Yu, Jing-Lun, Hsu, Hsien-Wen, Rau, Lih-Rou, Hsu, Fu-Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456792/
https://www.ncbi.nlm.nih.gov/pubmed/28773610
http://dx.doi.org/10.3390/ma9060487
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author Tsai, Shiao-Wen
Chang, Yu-Han
Yu, Jing-Lun
Hsu, Hsien-Wen
Rau, Lih-Rou
Hsu, Fu-Yin
author_facet Tsai, Shiao-Wen
Chang, Yu-Han
Yu, Jing-Lun
Hsu, Hsien-Wen
Rau, Lih-Rou
Hsu, Fu-Yin
author_sort Tsai, Shiao-Wen
collection PubMed
description A highly ordered, mesoporous (pore size 2~50 nm) bioactive glass (MBG) structure has a greater surface area and pore volume and excellent bone-forming bioactivity compared with traditional bioactive glasses (BGs). Hence, MBGs have been used in drug delivery and bone tissue engineering. MBGs can be developed as either a dense or porous block. Compared with a block, microbeads provide greater flexibility for filling different-shaped cavities and are suitable for culturing cells in vitro. In contrast, the fibrous structure of a scaffold has been shown to increase cell attachment and differentiation due to its ability to mimic the three-dimensional structure of natural extracellular matrices. Hence, the aim of this study is to fabricate MBG microbeads with a fibrous structure. First, a sol-gel/electrospinning technique was utilized to fabricate the MBG nanofiber (MBGNF) structure. Subsequently, the MBGNF microbeads (MFBs) were produced by an electrospraying technology. The results show that the diameter of the MFBs decreases when the applied voltage increases. The drug loading and release profiles and mechanisms of the MFBs were also evaluated. MFBs had a better drug entrapment efficiency, could reduce the burst release of tetracycline, and sustain the release over 10 days. Hence, the MFBs may be suitable drug carriers. In addition, the cellular attachment of MG63 osteoblast-like cells is significantly higher for MFBs than for glass microbeads after culturing for 4 h. The nanofibrous structure of MFBs could provide an appropriate environment for cellular spreading. Therefore, MFBs have great potential for use as a bone graft material in bone tissue engineering applications.
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spelling pubmed-54567922017-07-28 Preparation of Nanofibrous Structure of Mesoporous Bioactive Glass Microbeads for Biomedical Applications Tsai, Shiao-Wen Chang, Yu-Han Yu, Jing-Lun Hsu, Hsien-Wen Rau, Lih-Rou Hsu, Fu-Yin Materials (Basel) Article A highly ordered, mesoporous (pore size 2~50 nm) bioactive glass (MBG) structure has a greater surface area and pore volume and excellent bone-forming bioactivity compared with traditional bioactive glasses (BGs). Hence, MBGs have been used in drug delivery and bone tissue engineering. MBGs can be developed as either a dense or porous block. Compared with a block, microbeads provide greater flexibility for filling different-shaped cavities and are suitable for culturing cells in vitro. In contrast, the fibrous structure of a scaffold has been shown to increase cell attachment and differentiation due to its ability to mimic the three-dimensional structure of natural extracellular matrices. Hence, the aim of this study is to fabricate MBG microbeads with a fibrous structure. First, a sol-gel/electrospinning technique was utilized to fabricate the MBG nanofiber (MBGNF) structure. Subsequently, the MBGNF microbeads (MFBs) were produced by an electrospraying technology. The results show that the diameter of the MFBs decreases when the applied voltage increases. The drug loading and release profiles and mechanisms of the MFBs were also evaluated. MFBs had a better drug entrapment efficiency, could reduce the burst release of tetracycline, and sustain the release over 10 days. Hence, the MFBs may be suitable drug carriers. In addition, the cellular attachment of MG63 osteoblast-like cells is significantly higher for MFBs than for glass microbeads after culturing for 4 h. The nanofibrous structure of MFBs could provide an appropriate environment for cellular spreading. Therefore, MFBs have great potential for use as a bone graft material in bone tissue engineering applications. MDPI 2016-06-17 /pmc/articles/PMC5456792/ /pubmed/28773610 http://dx.doi.org/10.3390/ma9060487 Text en © 2016 by the authors; Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsai, Shiao-Wen
Chang, Yu-Han
Yu, Jing-Lun
Hsu, Hsien-Wen
Rau, Lih-Rou
Hsu, Fu-Yin
Preparation of Nanofibrous Structure of Mesoporous Bioactive Glass Microbeads for Biomedical Applications
title Preparation of Nanofibrous Structure of Mesoporous Bioactive Glass Microbeads for Biomedical Applications
title_full Preparation of Nanofibrous Structure of Mesoporous Bioactive Glass Microbeads for Biomedical Applications
title_fullStr Preparation of Nanofibrous Structure of Mesoporous Bioactive Glass Microbeads for Biomedical Applications
title_full_unstemmed Preparation of Nanofibrous Structure of Mesoporous Bioactive Glass Microbeads for Biomedical Applications
title_short Preparation of Nanofibrous Structure of Mesoporous Bioactive Glass Microbeads for Biomedical Applications
title_sort preparation of nanofibrous structure of mesoporous bioactive glass microbeads for biomedical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456792/
https://www.ncbi.nlm.nih.gov/pubmed/28773610
http://dx.doi.org/10.3390/ma9060487
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