Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering

Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of P...

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Detalles Bibliográficos
Autores principales: Hardy, John G., Torres-Rendon, Jose Guillermo, Leal-Egaña, Aldo, Walther, Andreas, Schlaad, Helmut, Cölfen, Helmut, Scheibel, Thomas R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456849/
https://www.ncbi.nlm.nih.gov/pubmed/28773681
http://dx.doi.org/10.3390/ma9070560
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author Hardy, John G.
Torres-Rendon, Jose Guillermo
Leal-Egaña, Aldo
Walther, Andreas
Schlaad, Helmut
Cölfen, Helmut
Scheibel, Thomas R.
author_facet Hardy, John G.
Torres-Rendon, Jose Guillermo
Leal-Egaña, Aldo
Walther, Andreas
Schlaad, Helmut
Cölfen, Helmut
Scheibel, Thomas R.
author_sort Hardy, John G.
collection PubMed
description Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.
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spelling pubmed-54568492017-07-28 Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering Hardy, John G. Torres-Rendon, Jose Guillermo Leal-Egaña, Aldo Walther, Andreas Schlaad, Helmut Cölfen, Helmut Scheibel, Thomas R. Materials (Basel) Communication Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering. MDPI 2016-07-11 /pmc/articles/PMC5456849/ /pubmed/28773681 http://dx.doi.org/10.3390/ma9070560 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Hardy, John G.
Torres-Rendon, Jose Guillermo
Leal-Egaña, Aldo
Walther, Andreas
Schlaad, Helmut
Cölfen, Helmut
Scheibel, Thomas R.
Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering
title Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering
title_full Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering
title_fullStr Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering
title_full_unstemmed Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering
title_short Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering
title_sort biomineralization of engineered spider silk protein-based composite materials for bone tissue engineering
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456849/
https://www.ncbi.nlm.nih.gov/pubmed/28773681
http://dx.doi.org/10.3390/ma9070560
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