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Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells

Background: To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP) ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing Ca...

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Autores principales: Braux, Julien, Velard, Frédéric, Guillaume, Christine, Jourdain, Marie-Laure, Gangloff, Sophie C., Jallot, Edouard, Nedelec, Jean-Marie, Laquerrière, Patrice, Laurent-Maquin, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456992/
https://www.ncbi.nlm.nih.gov/pubmed/28774105
http://dx.doi.org/10.3390/ma9120985
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author Braux, Julien
Velard, Frédéric
Guillaume, Christine
Jourdain, Marie-Laure
Gangloff, Sophie C.
Jallot, Edouard
Nedelec, Jean-Marie
Laquerrière, Patrice
Laurent-Maquin, Dominique
author_facet Braux, Julien
Velard, Frédéric
Guillaume, Christine
Jourdain, Marie-Laure
Gangloff, Sophie C.
Jallot, Edouard
Nedelec, Jean-Marie
Laquerrière, Patrice
Laurent-Maquin, Dominique
author_sort Braux, Julien
collection PubMed
description Background: To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP) ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing CaP ceramics were recently demonstrated for impacting cytokines’ secretion pattern of human primary monocytes. The present study focuses on the ability of strontium-containing CaP to control the human primary bone cell production of two major inflammatory and pro-osteoclastogenic mediators, namely MCP-1 and Gro-α, in response to ceramics particles. Methods: This in vitro study was performed using human primary osteoblasts in which their response to ceramics was evaluated by PCR arrays, antibody arrays were used for screening and real-time PCR and ELISA for more focused analyses. Results: Study of mRNA and protein expression highlights that human primary bone cells are able to produce these inflammatory mediators and reveal that the adjunction of CaP in the culture medium leads to their enhanced production. Importantly, the current work determines the down-regulating effect of strontium-substituted CaP on MCP-1 and Gro-α production. Conclusion: Our findings point out a new capability of strontium to modulate human primary bone cells’ communication with the immune system.
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spelling pubmed-54569922017-07-28 Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells Braux, Julien Velard, Frédéric Guillaume, Christine Jourdain, Marie-Laure Gangloff, Sophie C. Jallot, Edouard Nedelec, Jean-Marie Laquerrière, Patrice Laurent-Maquin, Dominique Materials (Basel) Article Background: To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP) ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing CaP ceramics were recently demonstrated for impacting cytokines’ secretion pattern of human primary monocytes. The present study focuses on the ability of strontium-containing CaP to control the human primary bone cell production of two major inflammatory and pro-osteoclastogenic mediators, namely MCP-1 and Gro-α, in response to ceramics particles. Methods: This in vitro study was performed using human primary osteoblasts in which their response to ceramics was evaluated by PCR arrays, antibody arrays were used for screening and real-time PCR and ELISA for more focused analyses. Results: Study of mRNA and protein expression highlights that human primary bone cells are able to produce these inflammatory mediators and reveal that the adjunction of CaP in the culture medium leads to their enhanced production. Importantly, the current work determines the down-regulating effect of strontium-substituted CaP on MCP-1 and Gro-α production. Conclusion: Our findings point out a new capability of strontium to modulate human primary bone cells’ communication with the immune system. MDPI 2016-12-05 /pmc/articles/PMC5456992/ /pubmed/28774105 http://dx.doi.org/10.3390/ma9120985 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Braux, Julien
Velard, Frédéric
Guillaume, Christine
Jourdain, Marie-Laure
Gangloff, Sophie C.
Jallot, Edouard
Nedelec, Jean-Marie
Laquerrière, Patrice
Laurent-Maquin, Dominique
Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells
title Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells
title_full Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells
title_fullStr Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells
title_full_unstemmed Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells
title_short Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells
title_sort strontium-substituted bioceramics particles: a new way to modulate mcp-1 and gro-α production by human primary osteoblastic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456992/
https://www.ncbi.nlm.nih.gov/pubmed/28774105
http://dx.doi.org/10.3390/ma9120985
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