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Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells
Background: To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP) ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing Ca...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456992/ https://www.ncbi.nlm.nih.gov/pubmed/28774105 http://dx.doi.org/10.3390/ma9120985 |
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author | Braux, Julien Velard, Frédéric Guillaume, Christine Jourdain, Marie-Laure Gangloff, Sophie C. Jallot, Edouard Nedelec, Jean-Marie Laquerrière, Patrice Laurent-Maquin, Dominique |
author_facet | Braux, Julien Velard, Frédéric Guillaume, Christine Jourdain, Marie-Laure Gangloff, Sophie C. Jallot, Edouard Nedelec, Jean-Marie Laquerrière, Patrice Laurent-Maquin, Dominique |
author_sort | Braux, Julien |
collection | PubMed |
description | Background: To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP) ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing CaP ceramics were recently demonstrated for impacting cytokines’ secretion pattern of human primary monocytes. The present study focuses on the ability of strontium-containing CaP to control the human primary bone cell production of two major inflammatory and pro-osteoclastogenic mediators, namely MCP-1 and Gro-α, in response to ceramics particles. Methods: This in vitro study was performed using human primary osteoblasts in which their response to ceramics was evaluated by PCR arrays, antibody arrays were used for screening and real-time PCR and ELISA for more focused analyses. Results: Study of mRNA and protein expression highlights that human primary bone cells are able to produce these inflammatory mediators and reveal that the adjunction of CaP in the culture medium leads to their enhanced production. Importantly, the current work determines the down-regulating effect of strontium-substituted CaP on MCP-1 and Gro-α production. Conclusion: Our findings point out a new capability of strontium to modulate human primary bone cells’ communication with the immune system. |
format | Online Article Text |
id | pubmed-5456992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54569922017-07-28 Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells Braux, Julien Velard, Frédéric Guillaume, Christine Jourdain, Marie-Laure Gangloff, Sophie C. Jallot, Edouard Nedelec, Jean-Marie Laquerrière, Patrice Laurent-Maquin, Dominique Materials (Basel) Article Background: To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP) ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing CaP ceramics were recently demonstrated for impacting cytokines’ secretion pattern of human primary monocytes. The present study focuses on the ability of strontium-containing CaP to control the human primary bone cell production of two major inflammatory and pro-osteoclastogenic mediators, namely MCP-1 and Gro-α, in response to ceramics particles. Methods: This in vitro study was performed using human primary osteoblasts in which their response to ceramics was evaluated by PCR arrays, antibody arrays were used for screening and real-time PCR and ELISA for more focused analyses. Results: Study of mRNA and protein expression highlights that human primary bone cells are able to produce these inflammatory mediators and reveal that the adjunction of CaP in the culture medium leads to their enhanced production. Importantly, the current work determines the down-regulating effect of strontium-substituted CaP on MCP-1 and Gro-α production. Conclusion: Our findings point out a new capability of strontium to modulate human primary bone cells’ communication with the immune system. MDPI 2016-12-05 /pmc/articles/PMC5456992/ /pubmed/28774105 http://dx.doi.org/10.3390/ma9120985 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Braux, Julien Velard, Frédéric Guillaume, Christine Jourdain, Marie-Laure Gangloff, Sophie C. Jallot, Edouard Nedelec, Jean-Marie Laquerrière, Patrice Laurent-Maquin, Dominique Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells |
title | Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells |
title_full | Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells |
title_fullStr | Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells |
title_full_unstemmed | Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells |
title_short | Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells |
title_sort | strontium-substituted bioceramics particles: a new way to modulate mcp-1 and gro-α production by human primary osteoblastic cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456992/ https://www.ncbi.nlm.nih.gov/pubmed/28774105 http://dx.doi.org/10.3390/ma9120985 |
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