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The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies

Despite our increasing understanding of zebrafish heart development and regeneration, there is limited information about the distribution of endothelial cells (ECs) in the adult zebrafish heart. Here, we investigate and compare the distribution of cardiac ECs (cECs) in adult mouse and zebrafish vent...

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Autores principales: Patra, Chinmoy, Kontarakis, Zacharias, Kaur, Harmandeep, Rayrikar, Amey, Mukherjee, Debanjan, Stainier, Didier Y. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457396/
https://www.ncbi.nlm.nih.gov/pubmed/28578380
http://dx.doi.org/10.1038/s41598-017-02461-1
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author Patra, Chinmoy
Kontarakis, Zacharias
Kaur, Harmandeep
Rayrikar, Amey
Mukherjee, Debanjan
Stainier, Didier Y. R.
author_facet Patra, Chinmoy
Kontarakis, Zacharias
Kaur, Harmandeep
Rayrikar, Amey
Mukherjee, Debanjan
Stainier, Didier Y. R.
author_sort Patra, Chinmoy
collection PubMed
description Despite our increasing understanding of zebrafish heart development and regeneration, there is limited information about the distribution of endothelial cells (ECs) in the adult zebrafish heart. Here, we investigate and compare the distribution of cardiac ECs (cECs) in adult mouse and zebrafish ventricles. Surprisingly, we find that (i) active coronary vessel growth is present in adult zebrafish, (ii) ~37 and ~39% of cells in the zebrafish heart are ECs and cardiomyocytes, respectively, a composition similar to that seen in mouse. However, we find that in zebrafish, ~36% of the ventricular tissue is covered with ECs, i.e., a substantially larger proportion than in mouse. Capitalising on the high abundance of cECs in zebrafish, we established a protocol to isolate them with high purity using fluorescent transgenic lines. Our approach eliminates side-effects due to antibody utilisation. Moreover, the isolated cECs maintained a high proliferation index even after three passages and were amenable to pharmacological treatments to study cEC migration in vitro. Such primary cultures will be a useful tool for supplementary in vitro studies on the accumulating zebrafish mutant lines as well as the screening of small molecule libraries on cardiac specific endothelial cells.
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spelling pubmed-54573962017-06-06 The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies Patra, Chinmoy Kontarakis, Zacharias Kaur, Harmandeep Rayrikar, Amey Mukherjee, Debanjan Stainier, Didier Y. R. Sci Rep Article Despite our increasing understanding of zebrafish heart development and regeneration, there is limited information about the distribution of endothelial cells (ECs) in the adult zebrafish heart. Here, we investigate and compare the distribution of cardiac ECs (cECs) in adult mouse and zebrafish ventricles. Surprisingly, we find that (i) active coronary vessel growth is present in adult zebrafish, (ii) ~37 and ~39% of cells in the zebrafish heart are ECs and cardiomyocytes, respectively, a composition similar to that seen in mouse. However, we find that in zebrafish, ~36% of the ventricular tissue is covered with ECs, i.e., a substantially larger proportion than in mouse. Capitalising on the high abundance of cECs in zebrafish, we established a protocol to isolate them with high purity using fluorescent transgenic lines. Our approach eliminates side-effects due to antibody utilisation. Moreover, the isolated cECs maintained a high proliferation index even after three passages and were amenable to pharmacological treatments to study cEC migration in vitro. Such primary cultures will be a useful tool for supplementary in vitro studies on the accumulating zebrafish mutant lines as well as the screening of small molecule libraries on cardiac specific endothelial cells. Nature Publishing Group UK 2017-06-02 /pmc/articles/PMC5457396/ /pubmed/28578380 http://dx.doi.org/10.1038/s41598-017-02461-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Patra, Chinmoy
Kontarakis, Zacharias
Kaur, Harmandeep
Rayrikar, Amey
Mukherjee, Debanjan
Stainier, Didier Y. R.
The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title_full The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title_fullStr The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title_full_unstemmed The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title_short The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
title_sort zebrafish ventricle: a hub of cardiac endothelial cells for in vitro cell behavior studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457396/
https://www.ncbi.nlm.nih.gov/pubmed/28578380
http://dx.doi.org/10.1038/s41598-017-02461-1
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