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Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects
BACKGROUND: Oxidative stress may aggravate symptoms of hemolytic anemias such as beta-thalassemia. FoxO3 activation results in resistance to oxidative stress in fibroblasts and neuronal cell cultures. OBJECTIVE: The purpose of this research was to study FoxO3 gene expression and oxidative status in...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Hematologia e Hemoterapia
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457465/ https://www.ncbi.nlm.nih.gov/pubmed/28577647 http://dx.doi.org/10.1016/j.bjhh.2017.01.005 |
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author | Lazarte, Sandra Stella Mónaco, María Eugenia Terán, Magdalena María Haro, Ana Cecilia Achem, Miryam Emilse Ledesma Issé, Blanca Alicia |
author_facet | Lazarte, Sandra Stella Mónaco, María Eugenia Terán, Magdalena María Haro, Ana Cecilia Achem, Miryam Emilse Ledesma Issé, Blanca Alicia |
author_sort | Lazarte, Sandra Stella |
collection | PubMed |
description | BACKGROUND: Oxidative stress may aggravate symptoms of hemolytic anemias such as beta-thalassemia. FoxO3 activation results in resistance to oxidative stress in fibroblasts and neuronal cell cultures. OBJECTIVE: The purpose of this research was to study FoxO3 gene expression and oxidative status in beta-thalassemia minor individuals. METHODS: Sixty-three subjects (42 apparently healthy individuals and 21 with beta-thalassemia minor) were analyzed at the Universidad Nacional de Tucumán, Argentina, between September 2013 and June 2014. A complete blood count, hemoglobin electrophoresis in alkaline pH and hemoglobin A(2) levels were quantified. Moreover, thiobarbituric acid reactive species, erythrocyte catalase activity and iron status were evaluated. Beta-thalassemia mutations were determined by real-time polymerase chain reaction. FoxO3 gene expression was investigated by real-time reverse transcription-polymerase chain reaction using mononuclear cells from peripheral blood. RESULTS: Subjects were grouped as children (≤12 years), and adult women and men. The analysis of erythrocyte catalase activity/hemoglobin ratio revealed a significant difference (p-value <0.05) between healthy and beta-thalassemia minor adults, but no significant difference was observed in the thiobarbituric acid reactive species levels and FoxO3 gene expression (p-value >0.05). Thiobarbituric acid reactive species and the erythrocyte catalase activity/hemoglobin ratio were not significantly different on comparing the type of beta-thalassemia mutation (β(0) or β(+)) present in carriers. CONCLUSIONS: The lack of systemic oxidative imbalance demonstrated by thiobarbituric acid reactive species is correlated to the observation of normal FoxO3 gene expression in mononuclear cells of peripheral blood. However, an imbalanced antioxidant state was shown by the erythrocyte catalase activity/hemoglobin ratio in beta-thalassemia minor carriers. It would be necessary to study FoxO3 gene expression in reticulocytes to elucidate the role of FoxO3 in this pathology. |
format | Online Article Text |
id | pubmed-5457465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Sociedade Brasileira de Hematologia e Hemoterapia |
record_format | MEDLINE/PubMed |
spelling | pubmed-54574652017-06-12 Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects Lazarte, Sandra Stella Mónaco, María Eugenia Terán, Magdalena María Haro, Ana Cecilia Achem, Miryam Emilse Ledesma Issé, Blanca Alicia Rev Bras Hematol Hemoter Original Article BACKGROUND: Oxidative stress may aggravate symptoms of hemolytic anemias such as beta-thalassemia. FoxO3 activation results in resistance to oxidative stress in fibroblasts and neuronal cell cultures. OBJECTIVE: The purpose of this research was to study FoxO3 gene expression and oxidative status in beta-thalassemia minor individuals. METHODS: Sixty-three subjects (42 apparently healthy individuals and 21 with beta-thalassemia minor) were analyzed at the Universidad Nacional de Tucumán, Argentina, between September 2013 and June 2014. A complete blood count, hemoglobin electrophoresis in alkaline pH and hemoglobin A(2) levels were quantified. Moreover, thiobarbituric acid reactive species, erythrocyte catalase activity and iron status were evaluated. Beta-thalassemia mutations were determined by real-time polymerase chain reaction. FoxO3 gene expression was investigated by real-time reverse transcription-polymerase chain reaction using mononuclear cells from peripheral blood. RESULTS: Subjects were grouped as children (≤12 years), and adult women and men. The analysis of erythrocyte catalase activity/hemoglobin ratio revealed a significant difference (p-value <0.05) between healthy and beta-thalassemia minor adults, but no significant difference was observed in the thiobarbituric acid reactive species levels and FoxO3 gene expression (p-value >0.05). Thiobarbituric acid reactive species and the erythrocyte catalase activity/hemoglobin ratio were not significantly different on comparing the type of beta-thalassemia mutation (β(0) or β(+)) present in carriers. CONCLUSIONS: The lack of systemic oxidative imbalance demonstrated by thiobarbituric acid reactive species is correlated to the observation of normal FoxO3 gene expression in mononuclear cells of peripheral blood. However, an imbalanced antioxidant state was shown by the erythrocyte catalase activity/hemoglobin ratio in beta-thalassemia minor carriers. It would be necessary to study FoxO3 gene expression in reticulocytes to elucidate the role of FoxO3 in this pathology. Sociedade Brasileira de Hematologia e Hemoterapia 2017 2017-02-22 /pmc/articles/PMC5457465/ /pubmed/28577647 http://dx.doi.org/10.1016/j.bjhh.2017.01.005 Text en © 2017 Associaç˜ao Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Lazarte, Sandra Stella Mónaco, María Eugenia Terán, Magdalena María Haro, Ana Cecilia Achem, Miryam Emilse Ledesma Issé, Blanca Alicia Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects |
title | Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects |
title_full | Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects |
title_fullStr | Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects |
title_full_unstemmed | Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects |
title_short | Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects |
title_sort | foxo3 gene expression and oxidative status in beta-thalassemia minor subjects |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457465/ https://www.ncbi.nlm.nih.gov/pubmed/28577647 http://dx.doi.org/10.1016/j.bjhh.2017.01.005 |
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