Cargando…

Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects

BACKGROUND: Oxidative stress may aggravate symptoms of hemolytic anemias such as beta-thalassemia. FoxO3 activation results in resistance to oxidative stress in fibroblasts and neuronal cell cultures. OBJECTIVE: The purpose of this research was to study FoxO3 gene expression and oxidative status in...

Descripción completa

Detalles Bibliográficos
Autores principales: Lazarte, Sandra Stella, Mónaco, María Eugenia, Terán, Magdalena María, Haro, Ana Cecilia, Achem, Miryam Emilse Ledesma, Issé, Blanca Alicia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Hematologia e Hemoterapia 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457465/
https://www.ncbi.nlm.nih.gov/pubmed/28577647
http://dx.doi.org/10.1016/j.bjhh.2017.01.005
_version_ 1783241542216974336
author Lazarte, Sandra Stella
Mónaco, María Eugenia
Terán, Magdalena María
Haro, Ana Cecilia
Achem, Miryam Emilse Ledesma
Issé, Blanca Alicia
author_facet Lazarte, Sandra Stella
Mónaco, María Eugenia
Terán, Magdalena María
Haro, Ana Cecilia
Achem, Miryam Emilse Ledesma
Issé, Blanca Alicia
author_sort Lazarte, Sandra Stella
collection PubMed
description BACKGROUND: Oxidative stress may aggravate symptoms of hemolytic anemias such as beta-thalassemia. FoxO3 activation results in resistance to oxidative stress in fibroblasts and neuronal cell cultures. OBJECTIVE: The purpose of this research was to study FoxO3 gene expression and oxidative status in beta-thalassemia minor individuals. METHODS: Sixty-three subjects (42 apparently healthy individuals and 21 with beta-thalassemia minor) were analyzed at the Universidad Nacional de Tucumán, Argentina, between September 2013 and June 2014. A complete blood count, hemoglobin electrophoresis in alkaline pH and hemoglobin A(2) levels were quantified. Moreover, thiobarbituric acid reactive species, erythrocyte catalase activity and iron status were evaluated. Beta-thalassemia mutations were determined by real-time polymerase chain reaction. FoxO3 gene expression was investigated by real-time reverse transcription-polymerase chain reaction using mononuclear cells from peripheral blood. RESULTS: Subjects were grouped as children (≤12 years), and adult women and men. The analysis of erythrocyte catalase activity/hemoglobin ratio revealed a significant difference (p-value <0.05) between healthy and beta-thalassemia minor adults, but no significant difference was observed in the thiobarbituric acid reactive species levels and FoxO3 gene expression (p-value >0.05). Thiobarbituric acid reactive species and the erythrocyte catalase activity/hemoglobin ratio were not significantly different on comparing the type of beta-thalassemia mutation (β(0) or β(+)) present in carriers. CONCLUSIONS: The lack of systemic oxidative imbalance demonstrated by thiobarbituric acid reactive species is correlated to the observation of normal FoxO3 gene expression in mononuclear cells of peripheral blood. However, an imbalanced antioxidant state was shown by the erythrocyte catalase activity/hemoglobin ratio in beta-thalassemia minor carriers. It would be necessary to study FoxO3 gene expression in reticulocytes to elucidate the role of FoxO3 in this pathology.
format Online
Article
Text
id pubmed-5457465
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Sociedade Brasileira de Hematologia e Hemoterapia
record_format MEDLINE/PubMed
spelling pubmed-54574652017-06-12 Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects Lazarte, Sandra Stella Mónaco, María Eugenia Terán, Magdalena María Haro, Ana Cecilia Achem, Miryam Emilse Ledesma Issé, Blanca Alicia Rev Bras Hematol Hemoter Original Article BACKGROUND: Oxidative stress may aggravate symptoms of hemolytic anemias such as beta-thalassemia. FoxO3 activation results in resistance to oxidative stress in fibroblasts and neuronal cell cultures. OBJECTIVE: The purpose of this research was to study FoxO3 gene expression and oxidative status in beta-thalassemia minor individuals. METHODS: Sixty-three subjects (42 apparently healthy individuals and 21 with beta-thalassemia minor) were analyzed at the Universidad Nacional de Tucumán, Argentina, between September 2013 and June 2014. A complete blood count, hemoglobin electrophoresis in alkaline pH and hemoglobin A(2) levels were quantified. Moreover, thiobarbituric acid reactive species, erythrocyte catalase activity and iron status were evaluated. Beta-thalassemia mutations were determined by real-time polymerase chain reaction. FoxO3 gene expression was investigated by real-time reverse transcription-polymerase chain reaction using mononuclear cells from peripheral blood. RESULTS: Subjects were grouped as children (≤12 years), and adult women and men. The analysis of erythrocyte catalase activity/hemoglobin ratio revealed a significant difference (p-value <0.05) between healthy and beta-thalassemia minor adults, but no significant difference was observed in the thiobarbituric acid reactive species levels and FoxO3 gene expression (p-value >0.05). Thiobarbituric acid reactive species and the erythrocyte catalase activity/hemoglobin ratio were not significantly different on comparing the type of beta-thalassemia mutation (β(0) or β(+)) present in carriers. CONCLUSIONS: The lack of systemic oxidative imbalance demonstrated by thiobarbituric acid reactive species is correlated to the observation of normal FoxO3 gene expression in mononuclear cells of peripheral blood. However, an imbalanced antioxidant state was shown by the erythrocyte catalase activity/hemoglobin ratio in beta-thalassemia minor carriers. It would be necessary to study FoxO3 gene expression in reticulocytes to elucidate the role of FoxO3 in this pathology. Sociedade Brasileira de Hematologia e Hemoterapia 2017 2017-02-22 /pmc/articles/PMC5457465/ /pubmed/28577647 http://dx.doi.org/10.1016/j.bjhh.2017.01.005 Text en © 2017 Associaç˜ao Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Lazarte, Sandra Stella
Mónaco, María Eugenia
Terán, Magdalena María
Haro, Ana Cecilia
Achem, Miryam Emilse Ledesma
Issé, Blanca Alicia
Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects
title Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects
title_full Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects
title_fullStr Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects
title_full_unstemmed Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects
title_short Foxo3 gene expression and oxidative status in beta-thalassemia minor subjects
title_sort foxo3 gene expression and oxidative status in beta-thalassemia minor subjects
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457465/
https://www.ncbi.nlm.nih.gov/pubmed/28577647
http://dx.doi.org/10.1016/j.bjhh.2017.01.005
work_keys_str_mv AT lazartesandrastella foxo3geneexpressionandoxidativestatusinbetathalassemiaminorsubjects
AT monacomariaeugenia foxo3geneexpressionandoxidativestatusinbetathalassemiaminorsubjects
AT teranmagdalenamaria foxo3geneexpressionandoxidativestatusinbetathalassemiaminorsubjects
AT haroanacecilia foxo3geneexpressionandoxidativestatusinbetathalassemiaminorsubjects
AT achemmiryamemilseledesma foxo3geneexpressionandoxidativestatusinbetathalassemiaminorsubjects
AT isseblancaalicia foxo3geneexpressionandoxidativestatusinbetathalassemiaminorsubjects