Cargando…
Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea
BACKGROUND: Clostridium perfringens is an opportunistic human pathogen that causes necrotic enteritis, mild diarrhea, clostridial myonecrosis or gas gangrene, sepsis, etc. In this study, we aim to determine the pathogenesis of this bacterium at the genomic level. The genome of strain CBA7123 was seq...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457660/ https://www.ncbi.nlm.nih.gov/pubmed/28588654 http://dx.doi.org/10.1186/s13099-017-0181-1 |
_version_ | 1783241590381215744 |
---|---|
author | Kim, Yeon Bee Kim, Joon Yong Song, Hye Seon Lee, Changsu Kwon, Joseph Kang, Jisu Rhee, Jin-Kyu Jeong, Myeong Seon Nam, Young-Do Roh, Seong Woon |
author_facet | Kim, Yeon Bee Kim, Joon Yong Song, Hye Seon Lee, Changsu Kwon, Joseph Kang, Jisu Rhee, Jin-Kyu Jeong, Myeong Seon Nam, Young-Do Roh, Seong Woon |
author_sort | Kim, Yeon Bee |
collection | PubMed |
description | BACKGROUND: Clostridium perfringens is an opportunistic human pathogen that causes necrotic enteritis, mild diarrhea, clostridial myonecrosis or gas gangrene, sepsis, etc. In this study, we aim to determine the pathogenesis of this bacterium at the genomic level. The genome of strain CBA7123 was sequenced, and a comparative genomic analysis between strain CBA7123 and four other related C. perfringens strains was performed. RESULTS: The genome of strain CBA7123 consisted of one circular chromosome and one plasmid that were 3,088,370 and 46,640 bp long with 28.5 and 27.1 mol% G+C content, respectively. The genomic DNA was predicted to contain 2798 open reading frames (ORFs), 10 rRNA genes, and 94 tRNA genes. The genomic comparison analysis between the five strains revealed the distinctive virulence properties of strain CBA7123 by highlighting certain strain-specific genes. CONCLUSIONS: In this study, the C. perfringens CBA7123 genome was sequenced and compared with other C. perfringens genomes. Among the various genes sequenced, the detection of antimicrobial resistance genes and those encoding various virulence factors may extend the understanding of the pathogenesis of C. perfringens strains. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13099-017-0181-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5457660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54576602017-06-06 Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea Kim, Yeon Bee Kim, Joon Yong Song, Hye Seon Lee, Changsu Kwon, Joseph Kang, Jisu Rhee, Jin-Kyu Jeong, Myeong Seon Nam, Young-Do Roh, Seong Woon Gut Pathog Genome Report BACKGROUND: Clostridium perfringens is an opportunistic human pathogen that causes necrotic enteritis, mild diarrhea, clostridial myonecrosis or gas gangrene, sepsis, etc. In this study, we aim to determine the pathogenesis of this bacterium at the genomic level. The genome of strain CBA7123 was sequenced, and a comparative genomic analysis between strain CBA7123 and four other related C. perfringens strains was performed. RESULTS: The genome of strain CBA7123 consisted of one circular chromosome and one plasmid that were 3,088,370 and 46,640 bp long with 28.5 and 27.1 mol% G+C content, respectively. The genomic DNA was predicted to contain 2798 open reading frames (ORFs), 10 rRNA genes, and 94 tRNA genes. The genomic comparison analysis between the five strains revealed the distinctive virulence properties of strain CBA7123 by highlighting certain strain-specific genes. CONCLUSIONS: In this study, the C. perfringens CBA7123 genome was sequenced and compared with other C. perfringens genomes. Among the various genes sequenced, the detection of antimicrobial resistance genes and those encoding various virulence factors may extend the understanding of the pathogenesis of C. perfringens strains. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13099-017-0181-1) contains supplementary material, which is available to authorized users. BioMed Central 2017-06-02 /pmc/articles/PMC5457660/ /pubmed/28588654 http://dx.doi.org/10.1186/s13099-017-0181-1 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Genome Report Kim, Yeon Bee Kim, Joon Yong Song, Hye Seon Lee, Changsu Kwon, Joseph Kang, Jisu Rhee, Jin-Kyu Jeong, Myeong Seon Nam, Young-Do Roh, Seong Woon Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea |
title | Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea |
title_full | Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea |
title_fullStr | Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea |
title_full_unstemmed | Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea |
title_short | Complete genome sequence of Clostridium perfringens CBA7123 isolated from a faecal sample from Korea |
title_sort | complete genome sequence of clostridium perfringens cba7123 isolated from a faecal sample from korea |
topic | Genome Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457660/ https://www.ncbi.nlm.nih.gov/pubmed/28588654 http://dx.doi.org/10.1186/s13099-017-0181-1 |
work_keys_str_mv | AT kimyeonbee completegenomesequenceofclostridiumperfringenscba7123isolatedfromafaecalsamplefromkorea AT kimjoonyong completegenomesequenceofclostridiumperfringenscba7123isolatedfromafaecalsamplefromkorea AT songhyeseon completegenomesequenceofclostridiumperfringenscba7123isolatedfromafaecalsamplefromkorea AT leechangsu completegenomesequenceofclostridiumperfringenscba7123isolatedfromafaecalsamplefromkorea AT kwonjoseph completegenomesequenceofclostridiumperfringenscba7123isolatedfromafaecalsamplefromkorea AT kangjisu completegenomesequenceofclostridiumperfringenscba7123isolatedfromafaecalsamplefromkorea AT rheejinkyu completegenomesequenceofclostridiumperfringenscba7123isolatedfromafaecalsamplefromkorea AT jeongmyeongseon completegenomesequenceofclostridiumperfringenscba7123isolatedfromafaecalsamplefromkorea AT namyoungdo completegenomesequenceofclostridiumperfringenscba7123isolatedfromafaecalsamplefromkorea AT rohseongwoon completegenomesequenceofclostridiumperfringenscba7123isolatedfromafaecalsamplefromkorea |