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(1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR in bipolar disorder metabolic profiling

BACKGROUND: The objective of this study was to identify molecular alterations in the human blood serum related to bipolar disorder, using nuclear magnetic resonance (NMR) spectroscopy and chemometrics. METHODS: Metabolomic profiling, employing (1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR spectroscopy...

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Autores principales: Sethi, Sumit, Pedrini, Mariana, Rizzo, Lucas B., Zeni-Graiff, Maiara, Mas, Caroline Dal, Cassinelli, Ana Cláudia, Noto, Mariane N., Asevedo, Elson, Cordeiro, Quirino, Pontes, João G. M., Brasil, Antonio J. M., Lacerda, Acioly, Hayashi, Mirian A. F., Poppi, Ronei, Tasic, Ljubica, Brietzke, Elisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457743/
https://www.ncbi.nlm.nih.gov/pubmed/28447334
http://dx.doi.org/10.1186/s40345-017-0088-2
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author Sethi, Sumit
Pedrini, Mariana
Rizzo, Lucas B.
Zeni-Graiff, Maiara
Mas, Caroline Dal
Cassinelli, Ana Cláudia
Noto, Mariane N.
Asevedo, Elson
Cordeiro, Quirino
Pontes, João G. M.
Brasil, Antonio J. M.
Lacerda, Acioly
Hayashi, Mirian A. F.
Poppi, Ronei
Tasic, Ljubica
Brietzke, Elisa
author_facet Sethi, Sumit
Pedrini, Mariana
Rizzo, Lucas B.
Zeni-Graiff, Maiara
Mas, Caroline Dal
Cassinelli, Ana Cláudia
Noto, Mariane N.
Asevedo, Elson
Cordeiro, Quirino
Pontes, João G. M.
Brasil, Antonio J. M.
Lacerda, Acioly
Hayashi, Mirian A. F.
Poppi, Ronei
Tasic, Ljubica
Brietzke, Elisa
author_sort Sethi, Sumit
collection PubMed
description BACKGROUND: The objective of this study was to identify molecular alterations in the human blood serum related to bipolar disorder, using nuclear magnetic resonance (NMR) spectroscopy and chemometrics. METHODS: Metabolomic profiling, employing (1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR spectroscopy and chemometrics of human blood serum samples from patients with bipolar disorder (n = 26) compared with healthy volunteers (n = 50) was performed. RESULTS: The investigated groups presented distinct metabolic profiles, in which the main differential metabolites found in the serum sample of bipolar disorder patients compared with those from controls were lipids, lipid metabolism-related molecules (choline, myo-inositol), and some amino acids (N-acetyl-l-phenyl alanine, N-acetyl-l-aspartyl-l-glutamic acid, l-glutamine). In addition, amygdalin, α-ketoglutaric acid, and lipoamide, among other compounds, were also present or were significantly altered in the serum of bipolar disorder patients. The data presented herein suggest that some of these metabolites differentially distributed between the groups studied may be directly related to the bipolar disorder pathophysiology. CONCLUSIONS: The strategy employed here showed significant potential for exploring pathophysiological features and molecular pathways involved in bipolar disorder. Thus, our findings may contribute to pave the way for future studies aiming at identifying important potential biomarkers for bipolar disorder diagnosis or progression follow-up. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40345-017-0088-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-54577432017-06-19 (1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR in bipolar disorder metabolic profiling Sethi, Sumit Pedrini, Mariana Rizzo, Lucas B. Zeni-Graiff, Maiara Mas, Caroline Dal Cassinelli, Ana Cláudia Noto, Mariane N. Asevedo, Elson Cordeiro, Quirino Pontes, João G. M. Brasil, Antonio J. M. Lacerda, Acioly Hayashi, Mirian A. F. Poppi, Ronei Tasic, Ljubica Brietzke, Elisa Int J Bipolar Disord Research BACKGROUND: The objective of this study was to identify molecular alterations in the human blood serum related to bipolar disorder, using nuclear magnetic resonance (NMR) spectroscopy and chemometrics. METHODS: Metabolomic profiling, employing (1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR spectroscopy and chemometrics of human blood serum samples from patients with bipolar disorder (n = 26) compared with healthy volunteers (n = 50) was performed. RESULTS: The investigated groups presented distinct metabolic profiles, in which the main differential metabolites found in the serum sample of bipolar disorder patients compared with those from controls were lipids, lipid metabolism-related molecules (choline, myo-inositol), and some amino acids (N-acetyl-l-phenyl alanine, N-acetyl-l-aspartyl-l-glutamic acid, l-glutamine). In addition, amygdalin, α-ketoglutaric acid, and lipoamide, among other compounds, were also present or were significantly altered in the serum of bipolar disorder patients. The data presented herein suggest that some of these metabolites differentially distributed between the groups studied may be directly related to the bipolar disorder pathophysiology. CONCLUSIONS: The strategy employed here showed significant potential for exploring pathophysiological features and molecular pathways involved in bipolar disorder. Thus, our findings may contribute to pave the way for future studies aiming at identifying important potential biomarkers for bipolar disorder diagnosis or progression follow-up. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40345-017-0088-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-06-04 /pmc/articles/PMC5457743/ /pubmed/28447334 http://dx.doi.org/10.1186/s40345-017-0088-2 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Sethi, Sumit
Pedrini, Mariana
Rizzo, Lucas B.
Zeni-Graiff, Maiara
Mas, Caroline Dal
Cassinelli, Ana Cláudia
Noto, Mariane N.
Asevedo, Elson
Cordeiro, Quirino
Pontes, João G. M.
Brasil, Antonio J. M.
Lacerda, Acioly
Hayashi, Mirian A. F.
Poppi, Ronei
Tasic, Ljubica
Brietzke, Elisa
(1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR in bipolar disorder metabolic profiling
title (1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR in bipolar disorder metabolic profiling
title_full (1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR in bipolar disorder metabolic profiling
title_fullStr (1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR in bipolar disorder metabolic profiling
title_full_unstemmed (1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR in bipolar disorder metabolic profiling
title_short (1)H-NMR, (1)H-NMR T(2)-edited, and 2D-NMR in bipolar disorder metabolic profiling
title_sort (1)h-nmr, (1)h-nmr t(2)-edited, and 2d-nmr in bipolar disorder metabolic profiling
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457743/
https://www.ncbi.nlm.nih.gov/pubmed/28447334
http://dx.doi.org/10.1186/s40345-017-0088-2
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