Cargando…
The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse
Ionizing radiation may cause irreversible ovarian failure, which, therefore, calls for an effective radioprotective reagent. The aim of the present study was to evaluate the potential radioprotective effect of N-acetylcysteine (NAC) on ionizing radiation induced ovarian failure and loss of ovarian r...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457747/ https://www.ncbi.nlm.nih.gov/pubmed/28607932 http://dx.doi.org/10.1155/2017/4176170 |
_version_ | 1783241601661796352 |
---|---|
author | Gao, Wei Liang, Jin-Xiao Ma, Chi Dong, Jing-yin Yan, Qiu |
author_facet | Gao, Wei Liang, Jin-Xiao Ma, Chi Dong, Jing-yin Yan, Qiu |
author_sort | Gao, Wei |
collection | PubMed |
description | Ionizing radiation may cause irreversible ovarian failure, which, therefore, calls for an effective radioprotective reagent. The aim of the present study was to evaluate the potential radioprotective effect of N-acetylcysteine (NAC) on ionizing radiation induced ovarian failure and loss of ovarian reserve in mice. Kun-Ming mice were either exposed to X-irradiation (4 Gy), once, and/or treated with NAC (300 mg/kg), once daily for 7 days before X-irradiation. We examined the serum circulating hormone levels and the development of ovarian follicles as well as apoptosis, cell proliferation, and oxidative stress 24 hours after X-irradiation. In addition, morphological observations on the endometrial luminal epithelium and the fertility assessment were performed. We found that NAC successfully restored the ovarian and uterine function, enhanced the embryo implantation, improved the follicle development, and altered the abnormal hormone levels through reducing the oxidative stress and apoptosis level in granulosa cells while promoting the proliferation of granulosa cells. In conclusion, the radioprotective effect of NAC on mice ovary from X-irradiation was assessed, and our results suggested that NAC can be a potential radioprotector which is capable of preventing the ovarian failure occurrence and restoring the ovarian reserve. |
format | Online Article Text |
id | pubmed-5457747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-54577472017-06-12 The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse Gao, Wei Liang, Jin-Xiao Ma, Chi Dong, Jing-yin Yan, Qiu Biomed Res Int Research Article Ionizing radiation may cause irreversible ovarian failure, which, therefore, calls for an effective radioprotective reagent. The aim of the present study was to evaluate the potential radioprotective effect of N-acetylcysteine (NAC) on ionizing radiation induced ovarian failure and loss of ovarian reserve in mice. Kun-Ming mice were either exposed to X-irradiation (4 Gy), once, and/or treated with NAC (300 mg/kg), once daily for 7 days before X-irradiation. We examined the serum circulating hormone levels and the development of ovarian follicles as well as apoptosis, cell proliferation, and oxidative stress 24 hours after X-irradiation. In addition, morphological observations on the endometrial luminal epithelium and the fertility assessment were performed. We found that NAC successfully restored the ovarian and uterine function, enhanced the embryo implantation, improved the follicle development, and altered the abnormal hormone levels through reducing the oxidative stress and apoptosis level in granulosa cells while promoting the proliferation of granulosa cells. In conclusion, the radioprotective effect of NAC on mice ovary from X-irradiation was assessed, and our results suggested that NAC can be a potential radioprotector which is capable of preventing the ovarian failure occurrence and restoring the ovarian reserve. Hindawi 2017 2017-05-21 /pmc/articles/PMC5457747/ /pubmed/28607932 http://dx.doi.org/10.1155/2017/4176170 Text en Copyright © 2017 Wei Gao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gao, Wei Liang, Jin-Xiao Ma, Chi Dong, Jing-yin Yan, Qiu The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse |
title | The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse |
title_full | The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse |
title_fullStr | The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse |
title_full_unstemmed | The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse |
title_short | The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse |
title_sort | protective effect of n-acetylcysteine on ionizing radiation induced ovarian failure and loss of ovarian reserve in female mouse |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457747/ https://www.ncbi.nlm.nih.gov/pubmed/28607932 http://dx.doi.org/10.1155/2017/4176170 |
work_keys_str_mv | AT gaowei theprotectiveeffectofnacetylcysteineonionizingradiationinducedovarianfailureandlossofovarianreserveinfemalemouse AT liangjinxiao theprotectiveeffectofnacetylcysteineonionizingradiationinducedovarianfailureandlossofovarianreserveinfemalemouse AT machi theprotectiveeffectofnacetylcysteineonionizingradiationinducedovarianfailureandlossofovarianreserveinfemalemouse AT dongjingyin theprotectiveeffectofnacetylcysteineonionizingradiationinducedovarianfailureandlossofovarianreserveinfemalemouse AT yanqiu theprotectiveeffectofnacetylcysteineonionizingradiationinducedovarianfailureandlossofovarianreserveinfemalemouse AT gaowei protectiveeffectofnacetylcysteineonionizingradiationinducedovarianfailureandlossofovarianreserveinfemalemouse AT liangjinxiao protectiveeffectofnacetylcysteineonionizingradiationinducedovarianfailureandlossofovarianreserveinfemalemouse AT machi protectiveeffectofnacetylcysteineonionizingradiationinducedovarianfailureandlossofovarianreserveinfemalemouse AT dongjingyin protectiveeffectofnacetylcysteineonionizingradiationinducedovarianfailureandlossofovarianreserveinfemalemouse AT yanqiu protectiveeffectofnacetylcysteineonionizingradiationinducedovarianfailureandlossofovarianreserveinfemalemouse |