Cargando…
Combination of immunohistochemistry, FISH and RT-PCR shows high incidence of Xp11 translocation RCC: comparison of three different diagnostic methods
We evaluated the frequency of translocation renal cell carcinoma (RCC) by reverse transcription polymerase chain reaction (RT-PCR) and how well the TFE3 immunoreactivity is concordant with TFE3 gene translocation status proved by fluorescence in situ hybridization (FISH) assay and RT-PCR. TFE3 and C...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458165/ https://www.ncbi.nlm.nih.gov/pubmed/28415646 http://dx.doi.org/10.18632/oncotarget.16481 |
_version_ | 1783241700161880064 |
---|---|
author | Lee, Hyun Jung Shin, Dong Hoon Noh, Gyu You Kim, Young Keum Kim, Ahrong Shin, Nari Lee, Jung Hee Choi, Kyung Un Kim, Jee Yeon Lee, Chang Hun Sol, Mee Young Rha, Seo Hee Park, Sung Woo |
author_facet | Lee, Hyun Jung Shin, Dong Hoon Noh, Gyu You Kim, Young Keum Kim, Ahrong Shin, Nari Lee, Jung Hee Choi, Kyung Un Kim, Jee Yeon Lee, Chang Hun Sol, Mee Young Rha, Seo Hee Park, Sung Woo |
author_sort | Lee, Hyun Jung |
collection | PubMed |
description | We evaluated the frequency of translocation renal cell carcinoma (RCC) by reverse transcription polymerase chain reaction (RT-PCR) and how well the TFE3 immunoreactivity is concordant with TFE3 gene translocation status proved by fluorescence in situ hybridization (FISH) assay and RT-PCR. TFE3 and Cathepsin K expression was analyzed by immunohistochemistry in 185 RCC cases, and 48 cases either of more than weak expression of TFE3 or of positivity for Cathepsin K were done for FISH analysis and RT-PCR. All the RT-PCR positive cases were confirmed by cloning and sequencing. Of the 14 cases with strong nuclear TFE3 expression, 12 showed a break-apart signal by FISH. ASPL- and PRCC-TFE3 translocations were detected in 13 and one case, respectively, by RT-PCR. Of 21 cases with weak TFE3 expression, five were translocation-positive by FISH. ASPL-, PRCC-, and PSF-TFE3 translocations were detected by RT-PCR (n=3, 3, and 1, respectively). All 13 TFE3-negative/cathepsin K-positive cases were negative by FISH and two each harbored ASPL- and PRCC-TFE3 translocations that were detected by RT-PCR. A high rate of TFE3 immunoreactivity (8.6%) was confirmed by RT-PCR (13.5%) and FISH (9.7%). Higher translocation rate of RT-PCR means RT-PCR detected translocation in TFE3 weak expression group and only cathepsin K positive group more specifically than FISH. Thus, RT-PCR would complement FISH analysis for detecting translocation RCC with fusion partners. |
format | Online Article Text |
id | pubmed-5458165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-54581652017-06-08 Combination of immunohistochemistry, FISH and RT-PCR shows high incidence of Xp11 translocation RCC: comparison of three different diagnostic methods Lee, Hyun Jung Shin, Dong Hoon Noh, Gyu You Kim, Young Keum Kim, Ahrong Shin, Nari Lee, Jung Hee Choi, Kyung Un Kim, Jee Yeon Lee, Chang Hun Sol, Mee Young Rha, Seo Hee Park, Sung Woo Oncotarget Research Paper: Pathology We evaluated the frequency of translocation renal cell carcinoma (RCC) by reverse transcription polymerase chain reaction (RT-PCR) and how well the TFE3 immunoreactivity is concordant with TFE3 gene translocation status proved by fluorescence in situ hybridization (FISH) assay and RT-PCR. TFE3 and Cathepsin K expression was analyzed by immunohistochemistry in 185 RCC cases, and 48 cases either of more than weak expression of TFE3 or of positivity for Cathepsin K were done for FISH analysis and RT-PCR. All the RT-PCR positive cases were confirmed by cloning and sequencing. Of the 14 cases with strong nuclear TFE3 expression, 12 showed a break-apart signal by FISH. ASPL- and PRCC-TFE3 translocations were detected in 13 and one case, respectively, by RT-PCR. Of 21 cases with weak TFE3 expression, five were translocation-positive by FISH. ASPL-, PRCC-, and PSF-TFE3 translocations were detected by RT-PCR (n=3, 3, and 1, respectively). All 13 TFE3-negative/cathepsin K-positive cases were negative by FISH and two each harbored ASPL- and PRCC-TFE3 translocations that were detected by RT-PCR. A high rate of TFE3 immunoreactivity (8.6%) was confirmed by RT-PCR (13.5%) and FISH (9.7%). Higher translocation rate of RT-PCR means RT-PCR detected translocation in TFE3 weak expression group and only cathepsin K positive group more specifically than FISH. Thus, RT-PCR would complement FISH analysis for detecting translocation RCC with fusion partners. Impact Journals LLC 2017-03-22 /pmc/articles/PMC5458165/ /pubmed/28415646 http://dx.doi.org/10.18632/oncotarget.16481 Text en Copyright: © 2017 Lee et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper: Pathology Lee, Hyun Jung Shin, Dong Hoon Noh, Gyu You Kim, Young Keum Kim, Ahrong Shin, Nari Lee, Jung Hee Choi, Kyung Un Kim, Jee Yeon Lee, Chang Hun Sol, Mee Young Rha, Seo Hee Park, Sung Woo Combination of immunohistochemistry, FISH and RT-PCR shows high incidence of Xp11 translocation RCC: comparison of three different diagnostic methods |
title | Combination of immunohistochemistry, FISH and RT-PCR shows high incidence of Xp11 translocation RCC: comparison of three different diagnostic methods |
title_full | Combination of immunohistochemistry, FISH and RT-PCR shows high incidence of Xp11 translocation RCC: comparison of three different diagnostic methods |
title_fullStr | Combination of immunohistochemistry, FISH and RT-PCR shows high incidence of Xp11 translocation RCC: comparison of three different diagnostic methods |
title_full_unstemmed | Combination of immunohistochemistry, FISH and RT-PCR shows high incidence of Xp11 translocation RCC: comparison of three different diagnostic methods |
title_short | Combination of immunohistochemistry, FISH and RT-PCR shows high incidence of Xp11 translocation RCC: comparison of three different diagnostic methods |
title_sort | combination of immunohistochemistry, fish and rt-pcr shows high incidence of xp11 translocation rcc: comparison of three different diagnostic methods |
topic | Research Paper: Pathology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458165/ https://www.ncbi.nlm.nih.gov/pubmed/28415646 http://dx.doi.org/10.18632/oncotarget.16481 |
work_keys_str_mv | AT leehyunjung combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT shindonghoon combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT nohgyuyou combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT kimyoungkeum combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT kimahrong combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT shinnari combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT leejunghee combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT choikyungun combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT kimjeeyeon combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT leechanghun combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT solmeeyoung combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT rhaseohee combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods AT parksungwoo combinationofimmunohistochemistryfishandrtpcrshowshighincidenceofxp11translocationrcccomparisonofthreedifferentdiagnosticmethods |