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Exosomal microRNAs isolated from plasma of mesenteric veins linked to liver metastases in resected patients with colon cancer

Before reaching a peripheral vein (PV), miRNAs released by the tumor are diluted and dispersed throughout the body or even retained in a specific organ. We hypothesized that blood drawn from the tumor-draining vein could provide more homogeneous information than blood drawn from the PV as that blood...

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Autores principales: Monzo, Mariano, Santasusagna, Sandra, Moreno, Isabel, Martinez, Francisco, Hernáez, Raquel, Muñoz, Carmen, Castellano, Joan J., Moreno, Josep, Navarro, Alfons
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458173/
https://www.ncbi.nlm.nih.gov/pubmed/28415718
http://dx.doi.org/10.18632/oncotarget.16103
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author Monzo, Mariano
Santasusagna, Sandra
Moreno, Isabel
Martinez, Francisco
Hernáez, Raquel
Muñoz, Carmen
Castellano, Joan J.
Moreno, Josep
Navarro, Alfons
author_facet Monzo, Mariano
Santasusagna, Sandra
Moreno, Isabel
Martinez, Francisco
Hernáez, Raquel
Muñoz, Carmen
Castellano, Joan J.
Moreno, Josep
Navarro, Alfons
author_sort Monzo, Mariano
collection PubMed
description Before reaching a peripheral vein (PV), miRNAs released by the tumor are diluted and dispersed throughout the body or even retained in a specific organ. We hypothesized that blood drawn from the tumor-draining vein could provide more homogeneous information than blood drawn from the PV as that blood would contain all the biomarkers released by the tumor before they reach a potential metastatic site. We have profiled 754 miRNAs in 15 colon cancer plasma samples from the tumor-draining vein, the mesenteric vein (MV), identifying 13 microRNAs associated with relapse. The prognostic impact of these miRNAs were validated in 50 MV and 50 paired PV plasma samples of stage I-III colon cancer patients. Four miRNAs, let-7g, miR-15b, miR-155 and miR-328, were found overexpressed in MV compared to PV, and patients with high levels of those miRNAs in MV plasma had shorter time to relapse. Interestingly, in patients developing liver metastases, the exosomal cargo of miR-328 was much greater in MV than in PV plasma indicating a possible role of miR-328 in the development of liver metastases. Our results indicate that in colon cancer, the primary tumor releases high concentrations of miRNAs through the MV, and some of them are contained in tumor derived exosomes.
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spelling pubmed-54581732017-06-08 Exosomal microRNAs isolated from plasma of mesenteric veins linked to liver metastases in resected patients with colon cancer Monzo, Mariano Santasusagna, Sandra Moreno, Isabel Martinez, Francisco Hernáez, Raquel Muñoz, Carmen Castellano, Joan J. Moreno, Josep Navarro, Alfons Oncotarget Research Paper Before reaching a peripheral vein (PV), miRNAs released by the tumor are diluted and dispersed throughout the body or even retained in a specific organ. We hypothesized that blood drawn from the tumor-draining vein could provide more homogeneous information than blood drawn from the PV as that blood would contain all the biomarkers released by the tumor before they reach a potential metastatic site. We have profiled 754 miRNAs in 15 colon cancer plasma samples from the tumor-draining vein, the mesenteric vein (MV), identifying 13 microRNAs associated with relapse. The prognostic impact of these miRNAs were validated in 50 MV and 50 paired PV plasma samples of stage I-III colon cancer patients. Four miRNAs, let-7g, miR-15b, miR-155 and miR-328, were found overexpressed in MV compared to PV, and patients with high levels of those miRNAs in MV plasma had shorter time to relapse. Interestingly, in patients developing liver metastases, the exosomal cargo of miR-328 was much greater in MV than in PV plasma indicating a possible role of miR-328 in the development of liver metastases. Our results indicate that in colon cancer, the primary tumor releases high concentrations of miRNAs through the MV, and some of them are contained in tumor derived exosomes. Impact Journals LLC 2017-03-10 /pmc/articles/PMC5458173/ /pubmed/28415718 http://dx.doi.org/10.18632/oncotarget.16103 Text en Copyright: © 2017 Monzo et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Monzo, Mariano
Santasusagna, Sandra
Moreno, Isabel
Martinez, Francisco
Hernáez, Raquel
Muñoz, Carmen
Castellano, Joan J.
Moreno, Josep
Navarro, Alfons
Exosomal microRNAs isolated from plasma of mesenteric veins linked to liver metastases in resected patients with colon cancer
title Exosomal microRNAs isolated from plasma of mesenteric veins linked to liver metastases in resected patients with colon cancer
title_full Exosomal microRNAs isolated from plasma of mesenteric veins linked to liver metastases in resected patients with colon cancer
title_fullStr Exosomal microRNAs isolated from plasma of mesenteric veins linked to liver metastases in resected patients with colon cancer
title_full_unstemmed Exosomal microRNAs isolated from plasma of mesenteric veins linked to liver metastases in resected patients with colon cancer
title_short Exosomal microRNAs isolated from plasma of mesenteric veins linked to liver metastases in resected patients with colon cancer
title_sort exosomal micrornas isolated from plasma of mesenteric veins linked to liver metastases in resected patients with colon cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458173/
https://www.ncbi.nlm.nih.gov/pubmed/28415718
http://dx.doi.org/10.18632/oncotarget.16103
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