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Genetic diversity of Plasmodium Vivax revealed by the merozoite surface protein-1 icb5-6 fragment

BACKGROUND: Plasmodium vivax remains a potential cause of morbidity and mortality for people living in its endemic areas. Understanding the genetic diversity of P. vivax from different regions is valuable for studying population dynamics and tracing the origins of parasites. The PvMSP-1 gene is high...

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Autores principales: Ruan, Wei, Zhang, Ling-ling, Feng, Yan, Zhang, Xuan, Chen, Hua-liang, Lu, Qiao-yi, Yao, Li-nong, Hu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458480/
https://www.ncbi.nlm.nih.gov/pubmed/28578709
http://dx.doi.org/10.1186/s40249-017-0302-6
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author Ruan, Wei
Zhang, Ling-ling
Feng, Yan
Zhang, Xuan
Chen, Hua-liang
Lu, Qiao-yi
Yao, Li-nong
Hu, Wei
author_facet Ruan, Wei
Zhang, Ling-ling
Feng, Yan
Zhang, Xuan
Chen, Hua-liang
Lu, Qiao-yi
Yao, Li-nong
Hu, Wei
author_sort Ruan, Wei
collection PubMed
description BACKGROUND: Plasmodium vivax remains a potential cause of morbidity and mortality for people living in its endemic areas. Understanding the genetic diversity of P. vivax from different regions is valuable for studying population dynamics and tracing the origins of parasites. The PvMSP-1 gene is highly polymorphic and has been used as a marker in many P. vivax population studies. The aim of this study was to investigate the genetic diversity of the PvMSP-1 gene icb5-6 fragment and to provide more genetic polymorphism data for further studies on P. vivax population structure and tracking of the origin of clinical cases. METHODS: Nested PCR and sequencing of the PvMSP-1 icb5-6 marker were performed to obtain the nucleotide sequences of 95 P. vivax isolates collected from Zhejiang province, China. To investigate the genetic diversity of PvMSP-1, the 95 nucleotide sequences of the PvMSP-1 icb5-6 fragment were genotyped and analyzed using DnaSP v5, MEGA software. RESULTS: The 95 P. vivax isolates collected from Zhejiang province were either indigenous cases or imported cases from different regions around the world. A total of 95 sequences ranging from 390 to 460 bp were obtained. The 95 sequences were genotyped into four allele-types (Sal I, Belem, R-III and R-IV) and 17 unique haplotypes. R-III and Sal I were the predominant allele-types. The haplotype diversity (Hd) and nucleotide diversity (Pi) were estimated to be 0.729 and 0.062, indicating that the PvMSP-1 icb5-6 fragment had the highest level of polymorphism due to frequent recombination processes and single nucleotide polymorphism. The values of dN/dS and Tajima’s D both suggested neutral selection for the PvMSP-1icb5-6 fragment. In addition, a rare recombinant style of R-IV type was identified. CONCLUSIONS: This study presented high genetic diversity in the PvMSP-1 marker among P. vivax strains from around the world. The genetic data is valuable for expanding the polymorphism information on P. vivax, which could be helpful for further study on population dynamics and tracking the origin of P. vivax. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40249-017-0302-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-54584802017-06-07 Genetic diversity of Plasmodium Vivax revealed by the merozoite surface protein-1 icb5-6 fragment Ruan, Wei Zhang, Ling-ling Feng, Yan Zhang, Xuan Chen, Hua-liang Lu, Qiao-yi Yao, Li-nong Hu, Wei Infect Dis Poverty Short Report BACKGROUND: Plasmodium vivax remains a potential cause of morbidity and mortality for people living in its endemic areas. Understanding the genetic diversity of P. vivax from different regions is valuable for studying population dynamics and tracing the origins of parasites. The PvMSP-1 gene is highly polymorphic and has been used as a marker in many P. vivax population studies. The aim of this study was to investigate the genetic diversity of the PvMSP-1 gene icb5-6 fragment and to provide more genetic polymorphism data for further studies on P. vivax population structure and tracking of the origin of clinical cases. METHODS: Nested PCR and sequencing of the PvMSP-1 icb5-6 marker were performed to obtain the nucleotide sequences of 95 P. vivax isolates collected from Zhejiang province, China. To investigate the genetic diversity of PvMSP-1, the 95 nucleotide sequences of the PvMSP-1 icb5-6 fragment were genotyped and analyzed using DnaSP v5, MEGA software. RESULTS: The 95 P. vivax isolates collected from Zhejiang province were either indigenous cases or imported cases from different regions around the world. A total of 95 sequences ranging from 390 to 460 bp were obtained. The 95 sequences were genotyped into four allele-types (Sal I, Belem, R-III and R-IV) and 17 unique haplotypes. R-III and Sal I were the predominant allele-types. The haplotype diversity (Hd) and nucleotide diversity (Pi) were estimated to be 0.729 and 0.062, indicating that the PvMSP-1 icb5-6 fragment had the highest level of polymorphism due to frequent recombination processes and single nucleotide polymorphism. The values of dN/dS and Tajima’s D both suggested neutral selection for the PvMSP-1icb5-6 fragment. In addition, a rare recombinant style of R-IV type was identified. CONCLUSIONS: This study presented high genetic diversity in the PvMSP-1 marker among P. vivax strains from around the world. The genetic data is valuable for expanding the polymorphism information on P. vivax, which could be helpful for further study on population dynamics and tracking the origin of P. vivax. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40249-017-0302-6) contains supplementary material, which is available to authorized users. BioMed Central 2017-06-05 /pmc/articles/PMC5458480/ /pubmed/28578709 http://dx.doi.org/10.1186/s40249-017-0302-6 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Ruan, Wei
Zhang, Ling-ling
Feng, Yan
Zhang, Xuan
Chen, Hua-liang
Lu, Qiao-yi
Yao, Li-nong
Hu, Wei
Genetic diversity of Plasmodium Vivax revealed by the merozoite surface protein-1 icb5-6 fragment
title Genetic diversity of Plasmodium Vivax revealed by the merozoite surface protein-1 icb5-6 fragment
title_full Genetic diversity of Plasmodium Vivax revealed by the merozoite surface protein-1 icb5-6 fragment
title_fullStr Genetic diversity of Plasmodium Vivax revealed by the merozoite surface protein-1 icb5-6 fragment
title_full_unstemmed Genetic diversity of Plasmodium Vivax revealed by the merozoite surface protein-1 icb5-6 fragment
title_short Genetic diversity of Plasmodium Vivax revealed by the merozoite surface protein-1 icb5-6 fragment
title_sort genetic diversity of plasmodium vivax revealed by the merozoite surface protein-1 icb5-6 fragment
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458480/
https://www.ncbi.nlm.nih.gov/pubmed/28578709
http://dx.doi.org/10.1186/s40249-017-0302-6
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