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Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in Synechococcus sp. PCC 7002

BACKGROUND: Synechococcus sp. PCC 7002 (henceforth Synechococcus) is developing into a powerful synthetic biology chassis. In order to streamline the integration of genes into the Synechococcus chromosome, validation of neutral integration sites with optimization of the DNA transformation protocol p...

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Detalles Bibliográficos
Autores principales: Vogel, Anne Ilse Maria, Lale, Rahmi, Hohmann-Marriott, Martin Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458483/
https://www.ncbi.nlm.nih.gov/pubmed/28592992
http://dx.doi.org/10.1186/s13036-017-0061-8