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Cryo-electron microscopy of chromatin biology

The basic unit of chromatin, the nucleosome core particle (NCP), controls how DNA in eukaryotic cells is compacted, replicated and read. Since its discovery, biochemists have sought to understand how this protein–DNA complex can help to control so many diverse tasks. Recent electron-microscopy (EM)...

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Detalles Bibliográficos
Autores principales: Wilson, Marcus D., Costa, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458496/
https://www.ncbi.nlm.nih.gov/pubmed/28580916
http://dx.doi.org/10.1107/S2059798317004430
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author Wilson, Marcus D.
Costa, Alessandro
author_facet Wilson, Marcus D.
Costa, Alessandro
author_sort Wilson, Marcus D.
collection PubMed
description The basic unit of chromatin, the nucleosome core particle (NCP), controls how DNA in eukaryotic cells is compacted, replicated and read. Since its discovery, biochemists have sought to understand how this protein–DNA complex can help to control so many diverse tasks. Recent electron-microscopy (EM) studies on NCP-containing assemblies have helped to describe important chromatin transactions at a molecular level. With the implementation of recent technical advances in single-particle EM, our understanding of how nucleosomes are recognized and read looks to take a leap forward. In this review, the authors highlight recent advances in the architectural understanding of chromatin biology elucidated by EM.
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spelling pubmed-54584962017-06-27 Cryo-electron microscopy of chromatin biology Wilson, Marcus D. Costa, Alessandro Acta Crystallogr D Struct Biol Research Papers The basic unit of chromatin, the nucleosome core particle (NCP), controls how DNA in eukaryotic cells is compacted, replicated and read. Since its discovery, biochemists have sought to understand how this protein–DNA complex can help to control so many diverse tasks. Recent electron-microscopy (EM) studies on NCP-containing assemblies have helped to describe important chromatin transactions at a molecular level. With the implementation of recent technical advances in single-particle EM, our understanding of how nucleosomes are recognized and read looks to take a leap forward. In this review, the authors highlight recent advances in the architectural understanding of chromatin biology elucidated by EM. International Union of Crystallography 2017-04-20 /pmc/articles/PMC5458496/ /pubmed/28580916 http://dx.doi.org/10.1107/S2059798317004430 Text en © Wilson & Costa 2017 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/2.0/uk/
spellingShingle Research Papers
Wilson, Marcus D.
Costa, Alessandro
Cryo-electron microscopy of chromatin biology
title Cryo-electron microscopy of chromatin biology
title_full Cryo-electron microscopy of chromatin biology
title_fullStr Cryo-electron microscopy of chromatin biology
title_full_unstemmed Cryo-electron microscopy of chromatin biology
title_short Cryo-electron microscopy of chromatin biology
title_sort cryo-electron microscopy of chromatin biology
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458496/
https://www.ncbi.nlm.nih.gov/pubmed/28580916
http://dx.doi.org/10.1107/S2059798317004430
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