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CRISPR system for genome engineering: the application for autophagy study

CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR...

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Autores principales: Cui, Jianzhou, Chew, Shirley Jia Li, Shi, Yin, Gong, Zhiyuan, Shen, Han-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458674/
https://www.ncbi.nlm.nih.gov/pubmed/28288698
http://dx.doi.org/10.5483/BMBRep.2017.50.5.044
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author Cui, Jianzhou
Chew, Shirley Jia Li
Shi, Yin
Gong, Zhiyuan
Shen, Han-Ming
author_facet Cui, Jianzhou
Chew, Shirley Jia Li
Shi, Yin
Gong, Zhiyuan
Shen, Han-Ming
author_sort Cui, Jianzhou
collection PubMed
description CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research.
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spelling pubmed-54586742017-06-08 CRISPR system for genome engineering: the application for autophagy study Cui, Jianzhou Chew, Shirley Jia Li Shi, Yin Gong, Zhiyuan Shen, Han-Ming BMB Rep Invited Mini Review CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research. Korean Society for Biochemistry and Molecular Biology 2017-05 2017-05-31 /pmc/articles/PMC5458674/ /pubmed/28288698 http://dx.doi.org/10.5483/BMBRep.2017.50.5.044 Text en Copyright © 2017 by the The Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Invited Mini Review
Cui, Jianzhou
Chew, Shirley Jia Li
Shi, Yin
Gong, Zhiyuan
Shen, Han-Ming
CRISPR system for genome engineering: the application for autophagy study
title CRISPR system for genome engineering: the application for autophagy study
title_full CRISPR system for genome engineering: the application for autophagy study
title_fullStr CRISPR system for genome engineering: the application for autophagy study
title_full_unstemmed CRISPR system for genome engineering: the application for autophagy study
title_short CRISPR system for genome engineering: the application for autophagy study
title_sort crispr system for genome engineering: the application for autophagy study
topic Invited Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458674/
https://www.ncbi.nlm.nih.gov/pubmed/28288698
http://dx.doi.org/10.5483/BMBRep.2017.50.5.044
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