The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures

Incorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are located at the...

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Autores principales: Mir, B., Solés, X., González, C., Escaja, N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5459817/
https://www.ncbi.nlm.nih.gov/pubmed/28584239
http://dx.doi.org/10.1038/s41598-017-02723-y
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author Mir, B.
Solés, X.
González, C.
Escaja, N.
author_facet Mir, B.
Solés, X.
González, C.
Escaja, N.
author_sort Mir, B.
collection PubMed
description Incorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are located at the ends of intercalated C:C(+) stacks. When psC occupies central positions, the resulting i-motifs are only observed at low pH and psC:C(+) or psC:psC(+) hemiprotonated base pairs are formed instead of their neutral analogs. Overall, our results suggest that positively charged base pairs are necessary to stabilize this non-canonical DNA structure.
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spelling pubmed-54598172017-06-06 The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures Mir, B. Solés, X. González, C. Escaja, N. Sci Rep Article Incorporation of pseudoisocytidine (psC), a neutral analogue of protonated cytidine, in i-motifs has been studied by spectroscopic methods. Our results show that neutral psC:C base pairs can stabilize i-motifs at neutral pH, but the stabilization only occurs when psC:C base pairs are located at the ends of intercalated C:C(+) stacks. When psC occupies central positions, the resulting i-motifs are only observed at low pH and psC:C(+) or psC:psC(+) hemiprotonated base pairs are formed instead of their neutral analogs. Overall, our results suggest that positively charged base pairs are necessary to stabilize this non-canonical DNA structure. Nature Publishing Group UK 2017-06-05 /pmc/articles/PMC5459817/ /pubmed/28584239 http://dx.doi.org/10.1038/s41598-017-02723-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mir, B.
Solés, X.
González, C.
Escaja, N.
The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title_full The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title_fullStr The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title_full_unstemmed The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title_short The effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
title_sort effect of the neutral cytidine protonated analogue pseudoisocytidine on the stability of i-motif structures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5459817/
https://www.ncbi.nlm.nih.gov/pubmed/28584239
http://dx.doi.org/10.1038/s41598-017-02723-y
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