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Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)

Plant JAZ (Jasmonate ZIM-domain) proteins play versatile roles in multiple aspects of plant development and defense. However, little is known about the JAZ family in allotetraploid upland cotton (Gossypium hirsutum) so far. In this study, 30 non-redundant JAZ genes were identified in upland cotton t...

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Autores principales: Li, Wen, Xia, Xiao-Cong, Han, Li-Hong, Ni, Ping, Yan, Jing-Qiu, Tao, Miao, Huang, Geng-Qing, Li, Xue-Bao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5459830/
https://www.ncbi.nlm.nih.gov/pubmed/28584307
http://dx.doi.org/10.1038/s41598-017-03155-4
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author Li, Wen
Xia, Xiao-Cong
Han, Li-Hong
Ni, Ping
Yan, Jing-Qiu
Tao, Miao
Huang, Geng-Qing
Li, Xue-Bao
author_facet Li, Wen
Xia, Xiao-Cong
Han, Li-Hong
Ni, Ping
Yan, Jing-Qiu
Tao, Miao
Huang, Geng-Qing
Li, Xue-Bao
author_sort Li, Wen
collection PubMed
description Plant JAZ (Jasmonate ZIM-domain) proteins play versatile roles in multiple aspects of plant development and defense. However, little is known about the JAZ family in allotetraploid upland cotton (Gossypium hirsutum) so far. In this study, 30 non-redundant JAZ genes were identified in upland cotton through genome-wide screening. Phylogenetic analysis revealed that the 30 proteins in cotton JAZ family are further divided into five groups (I – V), and members in the same group share highly conserved motif structures. Subcellular localization assay demonstrated that GhJAZ proteins are localized in the cell nucleus. Quantitative RT-PCR analysis indicated that GhJAZs display different expression patterns in cotton tissues, and most of them could be induced by Jasmonic (JA). Furthermore, some GhJAZ genes are preferentially expressed in cotton ovules and fibers, and showed differential expression in ovules of wild type cotton and fiberless mutant (fl) during fiber initiation. GhJAZ proteins could interact with each other to form homodimer or heterodimer, and they also interacted with some JA signaling regulators and the proteins involved in cotton fiber initiation. Collectively, our data suggested that some GhJAZ proteins may play important roles in cotton fiber initiation and development by regulating JA signaling as well as some fiber-related proteins.
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spelling pubmed-54598302017-06-06 Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum) Li, Wen Xia, Xiao-Cong Han, Li-Hong Ni, Ping Yan, Jing-Qiu Tao, Miao Huang, Geng-Qing Li, Xue-Bao Sci Rep Article Plant JAZ (Jasmonate ZIM-domain) proteins play versatile roles in multiple aspects of plant development and defense. However, little is known about the JAZ family in allotetraploid upland cotton (Gossypium hirsutum) so far. In this study, 30 non-redundant JAZ genes were identified in upland cotton through genome-wide screening. Phylogenetic analysis revealed that the 30 proteins in cotton JAZ family are further divided into five groups (I – V), and members in the same group share highly conserved motif structures. Subcellular localization assay demonstrated that GhJAZ proteins are localized in the cell nucleus. Quantitative RT-PCR analysis indicated that GhJAZs display different expression patterns in cotton tissues, and most of them could be induced by Jasmonic (JA). Furthermore, some GhJAZ genes are preferentially expressed in cotton ovules and fibers, and showed differential expression in ovules of wild type cotton and fiberless mutant (fl) during fiber initiation. GhJAZ proteins could interact with each other to form homodimer or heterodimer, and they also interacted with some JA signaling regulators and the proteins involved in cotton fiber initiation. Collectively, our data suggested that some GhJAZ proteins may play important roles in cotton fiber initiation and development by regulating JA signaling as well as some fiber-related proteins. Nature Publishing Group UK 2017-06-05 /pmc/articles/PMC5459830/ /pubmed/28584307 http://dx.doi.org/10.1038/s41598-017-03155-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Wen
Xia, Xiao-Cong
Han, Li-Hong
Ni, Ping
Yan, Jing-Qiu
Tao, Miao
Huang, Geng-Qing
Li, Xue-Bao
Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)
title Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)
title_full Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)
title_fullStr Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)
title_full_unstemmed Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)
title_short Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)
title_sort genome-wide identification and characterization of jaz gene family in upland cotton (gossypium hirsutum)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5459830/
https://www.ncbi.nlm.nih.gov/pubmed/28584307
http://dx.doi.org/10.1038/s41598-017-03155-4
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