Regulation of ERG3, ERG6, and ERG11 Genes in Antifungal-Resistant isolates of Candida parapsilosis

BACKGROUND: Candida parapsilosis is one of the five common strains of yeasts involved in invasive candidiasis. The expression analysis of sterol biosynthesis pathway genes, which are associated with resistance, can assist the better understanding of antifungal resistance mechanisms. METHODS: The ant...

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Autores principales: Lotfali, Ensieh, Ghajari, Ali, Kordbacheh, Parivash, Zaini, Farideh, Mirhendi, Hossein, Mohammadi, Rasoul, Noorbakhsh, Fatemeh, Rezaie, Sassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute 2017
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5459943/
https://www.ncbi.nlm.nih.gov/pubmed/28176517
http://dx.doi.org/10.18869/acadpub.ibj.21.4.275
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author Lotfali, Ensieh
Ghajari, Ali
Kordbacheh, Parivash
Zaini, Farideh
Mirhendi, Hossein
Mohammadi, Rasoul
Noorbakhsh, Fatemeh
Rezaie, Sassan
author_facet Lotfali, Ensieh
Ghajari, Ali
Kordbacheh, Parivash
Zaini, Farideh
Mirhendi, Hossein
Mohammadi, Rasoul
Noorbakhsh, Fatemeh
Rezaie, Sassan
author_sort Lotfali, Ensieh
collection PubMed
description BACKGROUND: Candida parapsilosis is one of the five common strains of yeasts involved in invasive candidiasis. The expression analysis of sterol biosynthesis pathway genes, which are associated with resistance, can assist the better understanding of antifungal resistance mechanisms. METHODS: The antifungal susceptibility of 120 clinical C. parapsilosis isolates was examined. The changes in the gene expression related to resistance were analyzed. RESULTS: Eight strains were resistant to fluconazole (FLC), itraconazole (ITC), and amphotericin B (AMB). The regulation variations included increased mRNA levels of ERG3, ERG6, and ERG11 and decreased mRNA levels of ERG3 and ERG6 in response to FLC. ERG11 mRNA level increases in response to ITC and AMB. CONCLUSION: The mechanism of resistance to azoles in C. parapsilosis is very similar to C. Albicans. This feature may help to design new treatment strategy for candidiasis
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spelling pubmed-54599432017-07-01 Regulation of ERG3, ERG6, and ERG11 Genes in Antifungal-Resistant isolates of Candida parapsilosis Lotfali, Ensieh Ghajari, Ali Kordbacheh, Parivash Zaini, Farideh Mirhendi, Hossein Mohammadi, Rasoul Noorbakhsh, Fatemeh Rezaie, Sassan Iran Biomed J Short Communication BACKGROUND: Candida parapsilosis is one of the five common strains of yeasts involved in invasive candidiasis. The expression analysis of sterol biosynthesis pathway genes, which are associated with resistance, can assist the better understanding of antifungal resistance mechanisms. METHODS: The antifungal susceptibility of 120 clinical C. parapsilosis isolates was examined. The changes in the gene expression related to resistance were analyzed. RESULTS: Eight strains were resistant to fluconazole (FLC), itraconazole (ITC), and amphotericin B (AMB). The regulation variations included increased mRNA levels of ERG3, ERG6, and ERG11 and decreased mRNA levels of ERG3 and ERG6 in response to FLC. ERG11 mRNA level increases in response to ITC and AMB. CONCLUSION: The mechanism of resistance to azoles in C. parapsilosis is very similar to C. Albicans. This feature may help to design new treatment strategy for candidiasis Pasteur Institute 2017-07 /pmc/articles/PMC5459943/ /pubmed/28176517 http://dx.doi.org/10.18869/acadpub.ibj.21.4.275 Text en Copyright: © Iranian Biomedical Journal http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Lotfali, Ensieh
Ghajari, Ali
Kordbacheh, Parivash
Zaini, Farideh
Mirhendi, Hossein
Mohammadi, Rasoul
Noorbakhsh, Fatemeh
Rezaie, Sassan
Regulation of ERG3, ERG6, and ERG11 Genes in Antifungal-Resistant isolates of Candida parapsilosis
title Regulation of ERG3, ERG6, and ERG11 Genes in Antifungal-Resistant isolates of Candida parapsilosis
title_full Regulation of ERG3, ERG6, and ERG11 Genes in Antifungal-Resistant isolates of Candida parapsilosis
title_fullStr Regulation of ERG3, ERG6, and ERG11 Genes in Antifungal-Resistant isolates of Candida parapsilosis
title_full_unstemmed Regulation of ERG3, ERG6, and ERG11 Genes in Antifungal-Resistant isolates of Candida parapsilosis
title_short Regulation of ERG3, ERG6, and ERG11 Genes in Antifungal-Resistant isolates of Candida parapsilosis
title_sort regulation of erg3, erg6, and erg11 genes in antifungal-resistant isolates of candida parapsilosis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5459943/
https://www.ncbi.nlm.nih.gov/pubmed/28176517
http://dx.doi.org/10.18869/acadpub.ibj.21.4.275
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