Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression
Black yeast, Aureobasidium pullulans is extracellularly produced β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. In this study, using Glycine max cv. Kurosengoku (Kurosengoku soybeans), the production of β-glucan through fermentation of A. pullulans was evaluated, and the effects of A....
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460285/ https://www.ncbi.nlm.nih.gov/pubmed/28588201 http://dx.doi.org/10.1038/s41598-017-03053-9 |
_version_ | 1783242137570115584 |
---|---|
author | Muramatsu, Daisuke Okabe, Mitsuyasu Takaoka, Akinori Kida, Hiroshi Iwai, Atsushi |
author_facet | Muramatsu, Daisuke Okabe, Mitsuyasu Takaoka, Akinori Kida, Hiroshi Iwai, Atsushi |
author_sort | Muramatsu, Daisuke |
collection | PubMed |
description | Black yeast, Aureobasidium pullulans is extracellularly produced β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. In this study, using Glycine max cv. Kurosengoku (Kurosengoku soybeans), the production of β-glucan through fermentation of A. pullulans was evaluated, and the effects of A. pullulans cultured fluid (AP-CF) containing β-glucan made with Kurosengoku soybeans (kAP-CF) on a human monocyte derived cell line, Mono Mac 6 cells were investigated. Concentration of β-glucan in kAP-CF reached the same level as normal AP-CF. An anti-angiogenic protein, Thrombospondin-1 (THBS1) was effectively induced after the stimulation with kAP-CF for comparison with AP-CF. The THBS1 is also induced after stimulation with hot water extract of Kurosengoku soybeans (KS-E), while the combined stimulation of β-glucan with KS-E more effectively induced THBS1 than that with KS-E alone. These results suggest effects of A. pullulans-produced β-glucan on the enhancement of Kurosengoku soybean-induced THBS1 expression. |
format | Online Article Text |
id | pubmed-5460285 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54602852017-06-07 Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression Muramatsu, Daisuke Okabe, Mitsuyasu Takaoka, Akinori Kida, Hiroshi Iwai, Atsushi Sci Rep Article Black yeast, Aureobasidium pullulans is extracellularly produced β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. In this study, using Glycine max cv. Kurosengoku (Kurosengoku soybeans), the production of β-glucan through fermentation of A. pullulans was evaluated, and the effects of A. pullulans cultured fluid (AP-CF) containing β-glucan made with Kurosengoku soybeans (kAP-CF) on a human monocyte derived cell line, Mono Mac 6 cells were investigated. Concentration of β-glucan in kAP-CF reached the same level as normal AP-CF. An anti-angiogenic protein, Thrombospondin-1 (THBS1) was effectively induced after the stimulation with kAP-CF for comparison with AP-CF. The THBS1 is also induced after stimulation with hot water extract of Kurosengoku soybeans (KS-E), while the combined stimulation of β-glucan with KS-E more effectively induced THBS1 than that with KS-E alone. These results suggest effects of A. pullulans-produced β-glucan on the enhancement of Kurosengoku soybean-induced THBS1 expression. Nature Publishing Group UK 2017-06-06 /pmc/articles/PMC5460285/ /pubmed/28588201 http://dx.doi.org/10.1038/s41598-017-03053-9 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Muramatsu, Daisuke Okabe, Mitsuyasu Takaoka, Akinori Kida, Hiroshi Iwai, Atsushi Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression |
title | Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression |
title_full | Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression |
title_fullStr | Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression |
title_full_unstemmed | Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression |
title_short | Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression |
title_sort | aureobasidium pullulans produced β-glucan is effective to enhance kurosengoku soybean extract induced thrombospondin-1 expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460285/ https://www.ncbi.nlm.nih.gov/pubmed/28588201 http://dx.doi.org/10.1038/s41598-017-03053-9 |
work_keys_str_mv | AT muramatsudaisuke aureobasidiumpullulansproducedbglucaniseffectivetoenhancekurosengokusoybeanextractinducedthrombospondin1expression AT okabemitsuyasu aureobasidiumpullulansproducedbglucaniseffectivetoenhancekurosengokusoybeanextractinducedthrombospondin1expression AT takaokaakinori aureobasidiumpullulansproducedbglucaniseffectivetoenhancekurosengokusoybeanextractinducedthrombospondin1expression AT kidahiroshi aureobasidiumpullulansproducedbglucaniseffectivetoenhancekurosengokusoybeanextractinducedthrombospondin1expression AT iwaiatsushi aureobasidiumpullulansproducedbglucaniseffectivetoenhancekurosengokusoybeanextractinducedthrombospondin1expression |