Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression

Black yeast, Aureobasidium pullulans is extracellularly produced β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. In this study, using Glycine max cv. Kurosengoku (Kurosengoku soybeans), the production of β-glucan through fermentation of A. pullulans was evaluated, and the effects of A....

Descripción completa

Detalles Bibliográficos
Autores principales: Muramatsu, Daisuke, Okabe, Mitsuyasu, Takaoka, Akinori, Kida, Hiroshi, Iwai, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460285/
https://www.ncbi.nlm.nih.gov/pubmed/28588201
http://dx.doi.org/10.1038/s41598-017-03053-9
_version_ 1783242137570115584
author Muramatsu, Daisuke
Okabe, Mitsuyasu
Takaoka, Akinori
Kida, Hiroshi
Iwai, Atsushi
author_facet Muramatsu, Daisuke
Okabe, Mitsuyasu
Takaoka, Akinori
Kida, Hiroshi
Iwai, Atsushi
author_sort Muramatsu, Daisuke
collection PubMed
description Black yeast, Aureobasidium pullulans is extracellularly produced β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. In this study, using Glycine max cv. Kurosengoku (Kurosengoku soybeans), the production of β-glucan through fermentation of A. pullulans was evaluated, and the effects of A. pullulans cultured fluid (AP-CF) containing β-glucan made with Kurosengoku soybeans (kAP-CF) on a human monocyte derived cell line, Mono Mac 6 cells were investigated. Concentration of β-glucan in kAP-CF reached the same level as normal AP-CF. An anti-angiogenic protein, Thrombospondin-1 (THBS1) was effectively induced after the stimulation with kAP-CF for comparison with AP-CF. The THBS1 is also induced after stimulation with hot water extract of Kurosengoku soybeans (KS-E), while the combined stimulation of β-glucan with KS-E more effectively induced THBS1 than that with KS-E alone. These results suggest effects of A. pullulans-produced β-glucan on the enhancement of Kurosengoku soybean-induced THBS1 expression.
format Online
Article
Text
id pubmed-5460285
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54602852017-06-07 Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression Muramatsu, Daisuke Okabe, Mitsuyasu Takaoka, Akinori Kida, Hiroshi Iwai, Atsushi Sci Rep Article Black yeast, Aureobasidium pullulans is extracellularly produced β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. In this study, using Glycine max cv. Kurosengoku (Kurosengoku soybeans), the production of β-glucan through fermentation of A. pullulans was evaluated, and the effects of A. pullulans cultured fluid (AP-CF) containing β-glucan made with Kurosengoku soybeans (kAP-CF) on a human monocyte derived cell line, Mono Mac 6 cells were investigated. Concentration of β-glucan in kAP-CF reached the same level as normal AP-CF. An anti-angiogenic protein, Thrombospondin-1 (THBS1) was effectively induced after the stimulation with kAP-CF for comparison with AP-CF. The THBS1 is also induced after stimulation with hot water extract of Kurosengoku soybeans (KS-E), while the combined stimulation of β-glucan with KS-E more effectively induced THBS1 than that with KS-E alone. These results suggest effects of A. pullulans-produced β-glucan on the enhancement of Kurosengoku soybean-induced THBS1 expression. Nature Publishing Group UK 2017-06-06 /pmc/articles/PMC5460285/ /pubmed/28588201 http://dx.doi.org/10.1038/s41598-017-03053-9 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Muramatsu, Daisuke
Okabe, Mitsuyasu
Takaoka, Akinori
Kida, Hiroshi
Iwai, Atsushi
Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression
title Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression
title_full Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression
title_fullStr Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression
title_full_unstemmed Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression
title_short Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression
title_sort aureobasidium pullulans produced β-glucan is effective to enhance kurosengoku soybean extract induced thrombospondin-1 expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460285/
https://www.ncbi.nlm.nih.gov/pubmed/28588201
http://dx.doi.org/10.1038/s41598-017-03053-9
work_keys_str_mv AT muramatsudaisuke aureobasidiumpullulansproducedbglucaniseffectivetoenhancekurosengokusoybeanextractinducedthrombospondin1expression
AT okabemitsuyasu aureobasidiumpullulansproducedbglucaniseffectivetoenhancekurosengokusoybeanextractinducedthrombospondin1expression
AT takaokaakinori aureobasidiumpullulansproducedbglucaniseffectivetoenhancekurosengokusoybeanextractinducedthrombospondin1expression
AT kidahiroshi aureobasidiumpullulansproducedbglucaniseffectivetoenhancekurosengokusoybeanextractinducedthrombospondin1expression
AT iwaiatsushi aureobasidiumpullulansproducedbglucaniseffectivetoenhancekurosengokusoybeanextractinducedthrombospondin1expression