Cargando…
Molecular Targets and Associated Potential Pathways of Danlu Capsules in Hyperplasia of Mammary Glands Based on Systems Pharmacology
Hyperplasia of mammary glands (HMG) is common in middle-aged women. Danlu capsules (DLCs) can effectively relieve pain and improve clinical symptoms and are safe for treating HMG. However, the active substances in DLCs and the molecular mechanisms of DLCs in HMG remain unclear. This study identified...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460382/ https://www.ncbi.nlm.nih.gov/pubmed/28620417 http://dx.doi.org/10.1155/2017/1930598 |
_version_ | 1783242159203287040 |
---|---|
author | Huang, Jihan Tang, Haitao Cao, Sumin He, Yingchun Feng, Yibin Wang, Kun Zheng, Qingshan |
author_facet | Huang, Jihan Tang, Haitao Cao, Sumin He, Yingchun Feng, Yibin Wang, Kun Zheng, Qingshan |
author_sort | Huang, Jihan |
collection | PubMed |
description | Hyperplasia of mammary glands (HMG) is common in middle-aged women. Danlu capsules (DLCs) can effectively relieve pain and improve clinical symptoms and are safe for treating HMG. However, the active substances in DLCs and the molecular mechanisms of DLCs in HMG remain unclear. This study identified the bioactive compounds and delineated the molecular targets and potential pathways of DLCs by using a systems pharmacology approach. The candidate compounds were retrieved from the traditional Chinese medicine systems pharmacology (TCMSP) database and analysis platform. Each candidate's druggability was analyzed according to its oral bioavailability and drug-likeness indices. The candidate proteins and genes were extracted in the TCMSP and UniProt Knowledgebase, respectively. The potential pathways associated with the genes were identified by performing gene enrichment analysis with DAVID Bioinformatics Resources 6.7. A total of 603 compounds were obtained from DLCs, and 39 compounds and 66 targets associated with HMG were obtained. Gene enrichment analysis yielded 10 significant pathways with 34 targets. The integrated HMG pathway revealed that DLCs probably act in patients with HMG through multiple mechanisms of anti-inflammation, analgesic effects, and hormonal regulation. This study provides novel insights into the mechanisms of DLCs in HMG, from the molecular level to the pathway level. |
format | Online Article Text |
id | pubmed-5460382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-54603822017-06-15 Molecular Targets and Associated Potential Pathways of Danlu Capsules in Hyperplasia of Mammary Glands Based on Systems Pharmacology Huang, Jihan Tang, Haitao Cao, Sumin He, Yingchun Feng, Yibin Wang, Kun Zheng, Qingshan Evid Based Complement Alternat Med Research Article Hyperplasia of mammary glands (HMG) is common in middle-aged women. Danlu capsules (DLCs) can effectively relieve pain and improve clinical symptoms and are safe for treating HMG. However, the active substances in DLCs and the molecular mechanisms of DLCs in HMG remain unclear. This study identified the bioactive compounds and delineated the molecular targets and potential pathways of DLCs by using a systems pharmacology approach. The candidate compounds were retrieved from the traditional Chinese medicine systems pharmacology (TCMSP) database and analysis platform. Each candidate's druggability was analyzed according to its oral bioavailability and drug-likeness indices. The candidate proteins and genes were extracted in the TCMSP and UniProt Knowledgebase, respectively. The potential pathways associated with the genes were identified by performing gene enrichment analysis with DAVID Bioinformatics Resources 6.7. A total of 603 compounds were obtained from DLCs, and 39 compounds and 66 targets associated with HMG were obtained. Gene enrichment analysis yielded 10 significant pathways with 34 targets. The integrated HMG pathway revealed that DLCs probably act in patients with HMG through multiple mechanisms of anti-inflammation, analgesic effects, and hormonal regulation. This study provides novel insights into the mechanisms of DLCs in HMG, from the molecular level to the pathway level. Hindawi 2017 2017-05-23 /pmc/articles/PMC5460382/ /pubmed/28620417 http://dx.doi.org/10.1155/2017/1930598 Text en Copyright © 2017 Jihan Huang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Huang, Jihan Tang, Haitao Cao, Sumin He, Yingchun Feng, Yibin Wang, Kun Zheng, Qingshan Molecular Targets and Associated Potential Pathways of Danlu Capsules in Hyperplasia of Mammary Glands Based on Systems Pharmacology |
title | Molecular Targets and Associated Potential Pathways of Danlu Capsules in Hyperplasia of Mammary Glands Based on Systems Pharmacology |
title_full | Molecular Targets and Associated Potential Pathways of Danlu Capsules in Hyperplasia of Mammary Glands Based on Systems Pharmacology |
title_fullStr | Molecular Targets and Associated Potential Pathways of Danlu Capsules in Hyperplasia of Mammary Glands Based on Systems Pharmacology |
title_full_unstemmed | Molecular Targets and Associated Potential Pathways of Danlu Capsules in Hyperplasia of Mammary Glands Based on Systems Pharmacology |
title_short | Molecular Targets and Associated Potential Pathways of Danlu Capsules in Hyperplasia of Mammary Glands Based on Systems Pharmacology |
title_sort | molecular targets and associated potential pathways of danlu capsules in hyperplasia of mammary glands based on systems pharmacology |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460382/ https://www.ncbi.nlm.nih.gov/pubmed/28620417 http://dx.doi.org/10.1155/2017/1930598 |
work_keys_str_mv | AT huangjihan moleculartargetsandassociatedpotentialpathwaysofdanlucapsulesinhyperplasiaofmammaryglandsbasedonsystemspharmacology AT tanghaitao moleculartargetsandassociatedpotentialpathwaysofdanlucapsulesinhyperplasiaofmammaryglandsbasedonsystemspharmacology AT caosumin moleculartargetsandassociatedpotentialpathwaysofdanlucapsulesinhyperplasiaofmammaryglandsbasedonsystemspharmacology AT heyingchun moleculartargetsandassociatedpotentialpathwaysofdanlucapsulesinhyperplasiaofmammaryglandsbasedonsystemspharmacology AT fengyibin moleculartargetsandassociatedpotentialpathwaysofdanlucapsulesinhyperplasiaofmammaryglandsbasedonsystemspharmacology AT wangkun moleculartargetsandassociatedpotentialpathwaysofdanlucapsulesinhyperplasiaofmammaryglandsbasedonsystemspharmacology AT zhengqingshan moleculartargetsandassociatedpotentialpathwaysofdanlucapsulesinhyperplasiaofmammaryglandsbasedonsystemspharmacology |