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The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses
BACKGROUND: The mimetic compound OTR4120 may replace endogenous-degraded heparan sulfates that normally maintain the bioactivity of growth factors that are important for tissue repair. Herein, we investigated the effect of OTR4120 on the healing of normal colonic anastomoses. METHODS: We evaluated t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460427/ https://www.ncbi.nlm.nih.gov/pubmed/28620413 http://dx.doi.org/10.1155/2017/1078062 |
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author | Nerstrøm, Malene Krarup, Peter-Martin Jorgensen, Lars Nannestad Ågren, Magnus S. |
author_facet | Nerstrøm, Malene Krarup, Peter-Martin Jorgensen, Lars Nannestad Ågren, Magnus S. |
author_sort | Nerstrøm, Malene |
collection | PubMed |
description | BACKGROUND: The mimetic compound OTR4120 may replace endogenous-degraded heparan sulfates that normally maintain the bioactivity of growth factors that are important for tissue repair. Herein, we investigated the effect of OTR4120 on the healing of normal colonic anastomoses. METHODS: We evaluated the following two treatment groups of male Sprague Dawley rats (220–256 g): control-treated colonic anastomoses (n = 25) and OTR4120-treated colonic anastomoses (n = 25). We resected 10 mm of the left colon and then applied either saline alone (control) or OTR4120 (100 μg/mL) in saline to the colonic ends before an end-to-end single-layer anastomosis was constructed and again on the anastomosis before the abdomen and skin were closed. RESULTS: On postoperative day 3, the anastomotic breaking strengths were 1.47 ± 0.32 N (mean ± SD) in the control group and 1.52 ± 0.27 N in the OTR4120-treated animals (P = 0.622). We also found that the hydroxyproline concentration (indicator of collagen) in the anastomotic wounds did not differ (P = 0.571) between the two groups. CONCLUSIONS: Our data demonstrate that a single local application of OTR4120 intraoperatively did not increase the biomechanical strength of colonic anastomoses at the critical postoperative day 3 when the anastomoses are the weakest. |
format | Online Article Text |
id | pubmed-5460427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-54604272017-06-15 The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses Nerstrøm, Malene Krarup, Peter-Martin Jorgensen, Lars Nannestad Ågren, Magnus S. Gastroenterol Res Pract Research Article BACKGROUND: The mimetic compound OTR4120 may replace endogenous-degraded heparan sulfates that normally maintain the bioactivity of growth factors that are important for tissue repair. Herein, we investigated the effect of OTR4120 on the healing of normal colonic anastomoses. METHODS: We evaluated the following two treatment groups of male Sprague Dawley rats (220–256 g): control-treated colonic anastomoses (n = 25) and OTR4120-treated colonic anastomoses (n = 25). We resected 10 mm of the left colon and then applied either saline alone (control) or OTR4120 (100 μg/mL) in saline to the colonic ends before an end-to-end single-layer anastomosis was constructed and again on the anastomosis before the abdomen and skin were closed. RESULTS: On postoperative day 3, the anastomotic breaking strengths were 1.47 ± 0.32 N (mean ± SD) in the control group and 1.52 ± 0.27 N in the OTR4120-treated animals (P = 0.622). We also found that the hydroxyproline concentration (indicator of collagen) in the anastomotic wounds did not differ (P = 0.571) between the two groups. CONCLUSIONS: Our data demonstrate that a single local application of OTR4120 intraoperatively did not increase the biomechanical strength of colonic anastomoses at the critical postoperative day 3 when the anastomoses are the weakest. Hindawi 2017 2017-05-16 /pmc/articles/PMC5460427/ /pubmed/28620413 http://dx.doi.org/10.1155/2017/1078062 Text en Copyright © 2017 Malene Nerstrøm et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nerstrøm, Malene Krarup, Peter-Martin Jorgensen, Lars Nannestad Ågren, Magnus S. The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
title | The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
title_full | The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
title_fullStr | The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
title_full_unstemmed | The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
title_short | The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
title_sort | effect of a synthetic heparan sulfate on the healing of colonic anastomoses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460427/ https://www.ncbi.nlm.nih.gov/pubmed/28620413 http://dx.doi.org/10.1155/2017/1078062 |
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