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Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping

BACKGROUND: Alfalfa (Medicago sativa) is one of the most important legume forage species in China and many other countries of the world. It provides a quality source of proteins and minerals to animals. Genetic underpinnings for these important traits, however, are elusive. An alfalfa (M. sativa) as...

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Autores principales: Jia, Congjun, Wu, Xinming, Chen, Min, Wang, Yunqi, Liu, Xiqiang, Gong, Pan, Xu, Qingfang, Wang, Xuemin, Gao, Hongwen, Wang, Zan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460482/
https://www.ncbi.nlm.nih.gov/pubmed/28583066
http://dx.doi.org/10.1186/s12870-017-1047-x
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author Jia, Congjun
Wu, Xinming
Chen, Min
Wang, Yunqi
Liu, Xiqiang
Gong, Pan
Xu, Qingfang
Wang, Xuemin
Gao, Hongwen
Wang, Zan
author_facet Jia, Congjun
Wu, Xinming
Chen, Min
Wang, Yunqi
Liu, Xiqiang
Gong, Pan
Xu, Qingfang
Wang, Xuemin
Gao, Hongwen
Wang, Zan
author_sort Jia, Congjun
collection PubMed
description BACKGROUND: Alfalfa (Medicago sativa) is one of the most important legume forage species in China and many other countries of the world. It provides a quality source of proteins and minerals to animals. Genetic underpinnings for these important traits, however, are elusive. An alfalfa (M. sativa) association mapping study for six traits, namely crude protein (CP), rumen undegraded protein (RUP), and four mineral elements (Ca, K, Mg and P), was conducted in three consecutive years using a large collection encompassing 336 genotypes genotyped with 85 simple sequence repeat (SSR) markers. RESULTS: All the traits were significantly influenced by genotype, environment, and genotype × environment interaction. Eight-five significant associations (P < 0.005) were identified. Among these, five associations with Ca were repeatedly observed and six co-localized associations were identified. CONCLUSIONS: The identified marker alleles significantly associated with the traits provided important information for understanding genetic controls of alfalfa quality. The markers could be used in assisting selection for the individual traits in breeding populations for developing new alfalfa cultivars. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-017-1047-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-54604822017-06-07 Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping Jia, Congjun Wu, Xinming Chen, Min Wang, Yunqi Liu, Xiqiang Gong, Pan Xu, Qingfang Wang, Xuemin Gao, Hongwen Wang, Zan BMC Plant Biol Research Article BACKGROUND: Alfalfa (Medicago sativa) is one of the most important legume forage species in China and many other countries of the world. It provides a quality source of proteins and minerals to animals. Genetic underpinnings for these important traits, however, are elusive. An alfalfa (M. sativa) association mapping study for six traits, namely crude protein (CP), rumen undegraded protein (RUP), and four mineral elements (Ca, K, Mg and P), was conducted in three consecutive years using a large collection encompassing 336 genotypes genotyped with 85 simple sequence repeat (SSR) markers. RESULTS: All the traits were significantly influenced by genotype, environment, and genotype × environment interaction. Eight-five significant associations (P < 0.005) were identified. Among these, five associations with Ca were repeatedly observed and six co-localized associations were identified. CONCLUSIONS: The identified marker alleles significantly associated with the traits provided important information for understanding genetic controls of alfalfa quality. The markers could be used in assisting selection for the individual traits in breeding populations for developing new alfalfa cultivars. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-017-1047-x) contains supplementary material, which is available to authorized users. BioMed Central 2017-06-06 /pmc/articles/PMC5460482/ /pubmed/28583066 http://dx.doi.org/10.1186/s12870-017-1047-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Jia, Congjun
Wu, Xinming
Chen, Min
Wang, Yunqi
Liu, Xiqiang
Gong, Pan
Xu, Qingfang
Wang, Xuemin
Gao, Hongwen
Wang, Zan
Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping
title Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping
title_full Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping
title_fullStr Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping
title_full_unstemmed Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping
title_short Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping
title_sort identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (medicago sativa) using association mapping
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460482/
https://www.ncbi.nlm.nih.gov/pubmed/28583066
http://dx.doi.org/10.1186/s12870-017-1047-x
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