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A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples

BACKGROUND: RNA-sequencing (RNA-seq) has emerged as one of the most sensitive tool for gene expression analysis. Among the library preparation methods available, the standard poly(A) + enrichment provides a comprehensive, detailed, and accurate view of polyadenylated RNAs. However, on samples of sub...

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Detalles Bibliográficos
Autores principales:	Schuierer, Sven, Carbone, Walter, Knehr, Judith, Petitjean, Virginie, Fernandez, Anita, Sultan, Marc, Roma, Guglielmo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460543/ https://www.ncbi.nlm.nih.gov/pubmed/28583074 http://dx.doi.org/10.1186/s12864-017-3827-y

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