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Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro

The use of three dimensional in vitro systems in cancer research is a promising path for developing effective anticancer therapies. The aim of this study was to engineer a functional 3-D in vitro model of normal and cancerous cervical tissue.Normal epithelial and immortalized cervical epithelial car...

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Autores principales: Zuk, Anna Karolina, Wen, Xuesong, Dilworth, Stephen, Li, Dong, Ghali, Lucy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial Department of Journal of Biomedical Research 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460612/
https://www.ncbi.nlm.nih.gov/pubmed/28808216
http://dx.doi.org/10.7555/JBR.31.20160150
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author Zuk, Anna Karolina
Wen, Xuesong
Dilworth, Stephen
Li, Dong
Ghali, Lucy
author_facet Zuk, Anna Karolina
Wen, Xuesong
Dilworth, Stephen
Li, Dong
Ghali, Lucy
author_sort Zuk, Anna Karolina
collection PubMed
description The use of three dimensional in vitro systems in cancer research is a promising path for developing effective anticancer therapies. The aim of this study was to engineer a functional 3-D in vitro model of normal and cancerous cervical tissue.Normal epithelial and immortalized cervical epithelial carcinoma cell lines were used to construct 3-D artificial normal cervical and cervical cancerous tissues. De-epidermised dermis (DED) was used as a scaffold for both models. Morphological analyses were conducted by using hematoxylin and eosin staining and characteristics of the models were studied by analyzing the expression of different structural cytokeratins and differential protein marker Mad1 using immunohistochemical technique.Haematoxylin and eosin staining results showed that normal cervical tissue had multi epithelial layers while cancerous cervical tissue showed dysplastic changes. Immunohistochemistry staining results revealed that for normal cervix model cytokeratin 10 was expressed in the upper stratified layer of epithelium while cytokeratin 5 was expressed mainly in the middle and basal layer. Cytokeratin 19 was weakly expressed in a few basal cells. Cervical cancer model showed cytokeratin 19 expression in different epithelial layers and weak or no expression for cytokeratin 5 and cytokeratin 10. Mad1 expression was detected in some suprabasal cells.The 3-D in vitro models showed stratified epithelial layers and expressed the same types and patterns of differentiation marker proteins as seen in corresponding in vivo tissue in either normal cervical or cervical cancerous tissue. Findings imply that they can serve as functional normal and cervical cancer models.
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spelling pubmed-54606122017-06-13 Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro Zuk, Anna Karolina Wen, Xuesong Dilworth, Stephen Li, Dong Ghali, Lucy J Biomed Res Original Article The use of three dimensional in vitro systems in cancer research is a promising path for developing effective anticancer therapies. The aim of this study was to engineer a functional 3-D in vitro model of normal and cancerous cervical tissue.Normal epithelial and immortalized cervical epithelial carcinoma cell lines were used to construct 3-D artificial normal cervical and cervical cancerous tissues. De-epidermised dermis (DED) was used as a scaffold for both models. Morphological analyses were conducted by using hematoxylin and eosin staining and characteristics of the models were studied by analyzing the expression of different structural cytokeratins and differential protein marker Mad1 using immunohistochemical technique.Haematoxylin and eosin staining results showed that normal cervical tissue had multi epithelial layers while cancerous cervical tissue showed dysplastic changes. Immunohistochemistry staining results revealed that for normal cervix model cytokeratin 10 was expressed in the upper stratified layer of epithelium while cytokeratin 5 was expressed mainly in the middle and basal layer. Cytokeratin 19 was weakly expressed in a few basal cells. Cervical cancer model showed cytokeratin 19 expression in different epithelial layers and weak or no expression for cytokeratin 5 and cytokeratin 10. Mad1 expression was detected in some suprabasal cells.The 3-D in vitro models showed stratified epithelial layers and expressed the same types and patterns of differentiation marker proteins as seen in corresponding in vivo tissue in either normal cervical or cervical cancerous tissue. Findings imply that they can serve as functional normal and cervical cancer models. Editorial Department of Journal of Biomedical Research 2017 /pmc/articles/PMC5460612/ /pubmed/28808216 http://dx.doi.org/10.7555/JBR.31.20160150 Text en This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Original Article
Zuk, Anna Karolina
Wen, Xuesong
Dilworth, Stephen
Li, Dong
Ghali, Lucy
Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro
title Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro
title_full Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro
title_fullStr Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro
title_full_unstemmed Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro
title_short Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro
title_sort modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460612/
https://www.ncbi.nlm.nih.gov/pubmed/28808216
http://dx.doi.org/10.7555/JBR.31.20160150
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