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Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development
Heterostyly is a common floral polymorphism, but the proteomic basis of this trait is still largely unexplored. In this study, self- and cross-pollination of L-morph and S-morph flowers and comparison of embryo sac development in eggplant (Solanum melongena L.) suggested that lower fruit set from S-...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460878/ https://www.ncbi.nlm.nih.gov/pubmed/28586360 http://dx.doi.org/10.1371/journal.pone.0179018 |
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author | Wang, Yikui Liu, Ake Li, Wenjia Jiang, Yaqing Song, Shiwei Li, Yan Chen, Riyuan |
author_facet | Wang, Yikui Liu, Ake Li, Wenjia Jiang, Yaqing Song, Shiwei Li, Yan Chen, Riyuan |
author_sort | Wang, Yikui |
collection | PubMed |
description | Heterostyly is a common floral polymorphism, but the proteomic basis of this trait is still largely unexplored. In this study, self- and cross-pollination of L-morph and S-morph flowers and comparison of embryo sac development in eggplant (Solanum melongena L.) suggested that lower fruit set from S-morph flowers results from stigma-pollen incompatibility. To explore the molecular mechanism underlying heterostyly development, we conducted isobaric tags for relative and absolute quantification (iTRAQ) proteomic analysis of eggplant pistils for L- and S-morph flowers. A total of 5,259 distinct proteins were identified during heterostyly development. Compared S-morph flowers with L-morph, we discovered 57 and 184 differentially expressed proteins (DEPs) during flower development and maturity, respectively. Quantitative real time polymerase chain reactions were used for nine genes to verify DEPs from the iTRAQ approach. During flower development, DEPs were mainly involved in morphogenesis, biosynthetic processes, and metabolic pathways. At flower maturity, DEPs primarily participated in biosynthetic processes, metabolic pathways, and the formation of ribosomes and proteasomes. Additionally, some proteins associated with senescence and programmed cell death were found to be upregulated in S-morph pistils, which may lead to the lower fruit set in S-morph flowers. Although the exact roles of these related proteins are not yet known, this was the first attempt to use an iTRAQ approach to analyze proteomes of heterostylous eggplant flowers, and these results will provide insights into biochemical events taking place during the development of heterostyly. |
format | Online Article Text |
id | pubmed-5460878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54608782017-06-15 Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development Wang, Yikui Liu, Ake Li, Wenjia Jiang, Yaqing Song, Shiwei Li, Yan Chen, Riyuan PLoS One Research Article Heterostyly is a common floral polymorphism, but the proteomic basis of this trait is still largely unexplored. In this study, self- and cross-pollination of L-morph and S-morph flowers and comparison of embryo sac development in eggplant (Solanum melongena L.) suggested that lower fruit set from S-morph flowers results from stigma-pollen incompatibility. To explore the molecular mechanism underlying heterostyly development, we conducted isobaric tags for relative and absolute quantification (iTRAQ) proteomic analysis of eggplant pistils for L- and S-morph flowers. A total of 5,259 distinct proteins were identified during heterostyly development. Compared S-morph flowers with L-morph, we discovered 57 and 184 differentially expressed proteins (DEPs) during flower development and maturity, respectively. Quantitative real time polymerase chain reactions were used for nine genes to verify DEPs from the iTRAQ approach. During flower development, DEPs were mainly involved in morphogenesis, biosynthetic processes, and metabolic pathways. At flower maturity, DEPs primarily participated in biosynthetic processes, metabolic pathways, and the formation of ribosomes and proteasomes. Additionally, some proteins associated with senescence and programmed cell death were found to be upregulated in S-morph pistils, which may lead to the lower fruit set in S-morph flowers. Although the exact roles of these related proteins are not yet known, this was the first attempt to use an iTRAQ approach to analyze proteomes of heterostylous eggplant flowers, and these results will provide insights into biochemical events taking place during the development of heterostyly. Public Library of Science 2017-06-06 /pmc/articles/PMC5460878/ /pubmed/28586360 http://dx.doi.org/10.1371/journal.pone.0179018 Text en © 2017 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wang, Yikui Liu, Ake Li, Wenjia Jiang, Yaqing Song, Shiwei Li, Yan Chen, Riyuan Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development |
title | Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development |
title_full | Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development |
title_fullStr | Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development |
title_full_unstemmed | Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development |
title_short | Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development |
title_sort | comparative proteomic analysis of eggplant (solanum melongena l.) heterostylous pistil development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460878/ https://www.ncbi.nlm.nih.gov/pubmed/28586360 http://dx.doi.org/10.1371/journal.pone.0179018 |
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