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pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion

Streptococcus pneumoniae is the main cause of bacterial meningitis, a life-threating disease with a high case fatality rate despite treatment with antibiotics. Pneumococci cause meningitis by invading the blood and penetrating the blood–brain barrier (BBB). Using stimulated emission depletion (STED)...

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Autores principales: Iovino, Federico, Engelen-Lee, Joo-Yeon, Brouwer, Matthijs, van de Beek, Diederik, van der Ende, Arie, Valls Seron, Merche, Mellroth, Peter, Muschiol, Sandra, Bergstrand, Jan, Widengren, Jerker, Henriques-Normark, Birgitta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461002/
https://www.ncbi.nlm.nih.gov/pubmed/28515075
http://dx.doi.org/10.1084/jem.20161668
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author Iovino, Federico
Engelen-Lee, Joo-Yeon
Brouwer, Matthijs
van de Beek, Diederik
van der Ende, Arie
Valls Seron, Merche
Mellroth, Peter
Muschiol, Sandra
Bergstrand, Jan
Widengren, Jerker
Henriques-Normark, Birgitta
author_facet Iovino, Federico
Engelen-Lee, Joo-Yeon
Brouwer, Matthijs
van de Beek, Diederik
van der Ende, Arie
Valls Seron, Merche
Mellroth, Peter
Muschiol, Sandra
Bergstrand, Jan
Widengren, Jerker
Henriques-Normark, Birgitta
author_sort Iovino, Federico
collection PubMed
description Streptococcus pneumoniae is the main cause of bacterial meningitis, a life-threating disease with a high case fatality rate despite treatment with antibiotics. Pneumococci cause meningitis by invading the blood and penetrating the blood–brain barrier (BBB). Using stimulated emission depletion (STED) super-resolution microscopy of brain biopsies from patients who died of pneumococcal meningitis, we observe that pneumococci colocalize with the two BBB endothelial receptors: polymeric immunoglobulin receptor (pIgR) and platelet endothelial cell adhesion molecule (PECAM-1). We show that the major adhesin of the pneumococcal pilus-1, RrgA, binds both receptors, whereas the choline binding protein PspC binds, but to a lower extent, only pIgR. Using a bacteremia-derived meningitis model and mutant mice, as well as antibodies against the two receptors, we prevent pneumococcal entry into the brain and meningitis development. By adding antibodies to antibiotic (ceftriaxone)-treated mice, we further reduce the bacterial burden in the brain. Our data suggest that inhibition of pIgR and PECAM-1 has the potential to prevent pneumococcal meningitis.
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spelling pubmed-54610022017-06-07 pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion Iovino, Federico Engelen-Lee, Joo-Yeon Brouwer, Matthijs van de Beek, Diederik van der Ende, Arie Valls Seron, Merche Mellroth, Peter Muschiol, Sandra Bergstrand, Jan Widengren, Jerker Henriques-Normark, Birgitta J Exp Med Research Articles Streptococcus pneumoniae is the main cause of bacterial meningitis, a life-threating disease with a high case fatality rate despite treatment with antibiotics. Pneumococci cause meningitis by invading the blood and penetrating the blood–brain barrier (BBB). Using stimulated emission depletion (STED) super-resolution microscopy of brain biopsies from patients who died of pneumococcal meningitis, we observe that pneumococci colocalize with the two BBB endothelial receptors: polymeric immunoglobulin receptor (pIgR) and platelet endothelial cell adhesion molecule (PECAM-1). We show that the major adhesin of the pneumococcal pilus-1, RrgA, binds both receptors, whereas the choline binding protein PspC binds, but to a lower extent, only pIgR. Using a bacteremia-derived meningitis model and mutant mice, as well as antibodies against the two receptors, we prevent pneumococcal entry into the brain and meningitis development. By adding antibodies to antibiotic (ceftriaxone)-treated mice, we further reduce the bacterial burden in the brain. Our data suggest that inhibition of pIgR and PECAM-1 has the potential to prevent pneumococcal meningitis. The Rockefeller University Press 2017-06-05 /pmc/articles/PMC5461002/ /pubmed/28515075 http://dx.doi.org/10.1084/jem.20161668 Text en © 2017 Iovino et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Iovino, Federico
Engelen-Lee, Joo-Yeon
Brouwer, Matthijs
van de Beek, Diederik
van der Ende, Arie
Valls Seron, Merche
Mellroth, Peter
Muschiol, Sandra
Bergstrand, Jan
Widengren, Jerker
Henriques-Normark, Birgitta
pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion
title pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion
title_full pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion
title_fullStr pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion
title_full_unstemmed pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion
title_short pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion
title_sort pigr and pecam-1 bind to pneumococcal adhesins rrga and pspc mediating bacterial brain invasion
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461002/
https://www.ncbi.nlm.nih.gov/pubmed/28515075
http://dx.doi.org/10.1084/jem.20161668
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