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Spatial regulation of organelle release from myosin V transport by p21-activated kinases

Correct positioning of organelles is essential to eukaryotic cells. Molecular motors transport organelles to their proper destinations, yet little is known about the pathways that define these destinations. In Saccharomyces cerevisiae, the myosin V motor Myo2 binds the vacuole-specific adapter Vac17...

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Detalles Bibliográficos
Autores principales: Yau, Richard G., Wong, Sara, Weisman, Lois S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461012/
https://www.ncbi.nlm.nih.gov/pubmed/28495836
http://dx.doi.org/10.1083/jcb.201607020
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author Yau, Richard G.
Wong, Sara
Weisman, Lois S.
author_facet Yau, Richard G.
Wong, Sara
Weisman, Lois S.
author_sort Yau, Richard G.
collection PubMed
description Correct positioning of organelles is essential to eukaryotic cells. Molecular motors transport organelles to their proper destinations, yet little is known about the pathways that define these destinations. In Saccharomyces cerevisiae, the myosin V motor Myo2 binds the vacuole-specific adapter Vac17 to attach to the vacuole/lysosome and initiate transport. After arrival in the bud, Myo2 releases the vacuole, and Vac17 is degraded. However, the mechanisms that spatially regulate this release were not established. In this study, we report that the bud cortex is a landmark that signals a successful delivery of the vacuole to the bud. We demonstrate that upon arrival at the bud cortex, Vac17 is phosphorylated by Cla4. Cla4-dependent phosphorylation is required for the ubiquitylation and subsequent degradation of Vac17 and the release of the vacuole from Myo2. Our study reveals a critical step in the spatial regulation of myosin V–dependent organelle transport and may reveal common mechanisms for how molecular motors accurately deposit cargoes at the correct locations.
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spelling pubmed-54610122017-12-05 Spatial regulation of organelle release from myosin V transport by p21-activated kinases Yau, Richard G. Wong, Sara Weisman, Lois S. J Cell Biol Research Articles Correct positioning of organelles is essential to eukaryotic cells. Molecular motors transport organelles to their proper destinations, yet little is known about the pathways that define these destinations. In Saccharomyces cerevisiae, the myosin V motor Myo2 binds the vacuole-specific adapter Vac17 to attach to the vacuole/lysosome and initiate transport. After arrival in the bud, Myo2 releases the vacuole, and Vac17 is degraded. However, the mechanisms that spatially regulate this release were not established. In this study, we report that the bud cortex is a landmark that signals a successful delivery of the vacuole to the bud. We demonstrate that upon arrival at the bud cortex, Vac17 is phosphorylated by Cla4. Cla4-dependent phosphorylation is required for the ubiquitylation and subsequent degradation of Vac17 and the release of the vacuole from Myo2. Our study reveals a critical step in the spatial regulation of myosin V–dependent organelle transport and may reveal common mechanisms for how molecular motors accurately deposit cargoes at the correct locations. The Rockefeller University Press 2017-06-05 /pmc/articles/PMC5461012/ /pubmed/28495836 http://dx.doi.org/10.1083/jcb.201607020 Text en © 2017 Yau et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Yau, Richard G.
Wong, Sara
Weisman, Lois S.
Spatial regulation of organelle release from myosin V transport by p21-activated kinases
title Spatial regulation of organelle release from myosin V transport by p21-activated kinases
title_full Spatial regulation of organelle release from myosin V transport by p21-activated kinases
title_fullStr Spatial regulation of organelle release from myosin V transport by p21-activated kinases
title_full_unstemmed Spatial regulation of organelle release from myosin V transport by p21-activated kinases
title_short Spatial regulation of organelle release from myosin V transport by p21-activated kinases
title_sort spatial regulation of organelle release from myosin v transport by p21-activated kinases
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461012/
https://www.ncbi.nlm.nih.gov/pubmed/28495836
http://dx.doi.org/10.1083/jcb.201607020
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