Cargando…

Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules

The initial kinetochore (KT) encounter with a spindle microtubule (MT; KT capture) is one of the rate-limiting steps in establishing proper KT–MT interaction during mitosis. KT capture is facilitated by multiple factors, such as MT extension in various directions, KT diffusion, and MT pivoting. In a...

Descripción completa

Detalles Bibliográficos
Autores principales: Vasileva, Vanya, Gierlinski, Marek, Yue, Zuojun, O’Reilly, Nicola, Kitamura, Etsushi, Tanaka, Tomoyuki U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461016/
https://www.ncbi.nlm.nih.gov/pubmed/28446512
http://dx.doi.org/10.1083/jcb.201608122
_version_ 1783242271089491968
author Vasileva, Vanya
Gierlinski, Marek
Yue, Zuojun
O’Reilly, Nicola
Kitamura, Etsushi
Tanaka, Tomoyuki U.
author_facet Vasileva, Vanya
Gierlinski, Marek
Yue, Zuojun
O’Reilly, Nicola
Kitamura, Etsushi
Tanaka, Tomoyuki U.
author_sort Vasileva, Vanya
collection PubMed
description The initial kinetochore (KT) encounter with a spindle microtubule (MT; KT capture) is one of the rate-limiting steps in establishing proper KT–MT interaction during mitosis. KT capture is facilitated by multiple factors, such as MT extension in various directions, KT diffusion, and MT pivoting. In addition, KTs generate short MTs, which subsequently interact with a spindle MT. KT-derived MTs may facilitate KT capture, but their contribution is elusive. In this study, we find that Stu1 recruits Stu2 to budding yeast KTs, which promotes MT generation there. By removing Stu2 specifically from KTs, we show that KT-derived MTs shorten the half-life of noncaptured KTs from 48–49 s to 28–34 s. Using computational simulation, we found that multiple factors facilitate KT capture redundantly or synergistically. In particular, KT-derived MTs play important roles both by making a significant contribution on their own and by synergistically enhancing the effects of KT diffusion and MT pivoting. Our study reveals fundamental mechanisms facilitating the initial KT encounter with spindle MTs.
format Online
Article
Text
id pubmed-5461016
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-54610162017-06-07 Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules Vasileva, Vanya Gierlinski, Marek Yue, Zuojun O’Reilly, Nicola Kitamura, Etsushi Tanaka, Tomoyuki U. J Cell Biol Research Articles The initial kinetochore (KT) encounter with a spindle microtubule (MT; KT capture) is one of the rate-limiting steps in establishing proper KT–MT interaction during mitosis. KT capture is facilitated by multiple factors, such as MT extension in various directions, KT diffusion, and MT pivoting. In addition, KTs generate short MTs, which subsequently interact with a spindle MT. KT-derived MTs may facilitate KT capture, but their contribution is elusive. In this study, we find that Stu1 recruits Stu2 to budding yeast KTs, which promotes MT generation there. By removing Stu2 specifically from KTs, we show that KT-derived MTs shorten the half-life of noncaptured KTs from 48–49 s to 28–34 s. Using computational simulation, we found that multiple factors facilitate KT capture redundantly or synergistically. In particular, KT-derived MTs play important roles both by making a significant contribution on their own and by synergistically enhancing the effects of KT diffusion and MT pivoting. Our study reveals fundamental mechanisms facilitating the initial KT encounter with spindle MTs. The Rockefeller University Press 2017-06-05 /pmc/articles/PMC5461016/ /pubmed/28446512 http://dx.doi.org/10.1083/jcb.201608122 Text en © 2017 Vasileva et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Vasileva, Vanya
Gierlinski, Marek
Yue, Zuojun
O’Reilly, Nicola
Kitamura, Etsushi
Tanaka, Tomoyuki U.
Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules
title Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules
title_full Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules
title_fullStr Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules
title_full_unstemmed Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules
title_short Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules
title_sort molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461016/
https://www.ncbi.nlm.nih.gov/pubmed/28446512
http://dx.doi.org/10.1083/jcb.201608122
work_keys_str_mv AT vasilevavanya molecularmechanismsfacilitatingtheinitialkinetochoreencounterwithspindlemicrotubules
AT gierlinskimarek molecularmechanismsfacilitatingtheinitialkinetochoreencounterwithspindlemicrotubules
AT yuezuojun molecularmechanismsfacilitatingtheinitialkinetochoreencounterwithspindlemicrotubules
AT oreillynicola molecularmechanismsfacilitatingtheinitialkinetochoreencounterwithspindlemicrotubules
AT kitamuraetsushi molecularmechanismsfacilitatingtheinitialkinetochoreencounterwithspindlemicrotubules
AT tanakatomoyukiu molecularmechanismsfacilitatingtheinitialkinetochoreencounterwithspindlemicrotubules