Cargando…
Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells
Minus end–directed cargo transport along microtubules (MTs) is exclusively driven by the molecular motor dynein in a wide variety of cell types. Interestingly, during evolution, plants have lost the genes encoding dynein; the MT motors that compensate for dynein function are unknown. Here, we show t...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461021/ https://www.ncbi.nlm.nih.gov/pubmed/28442535 http://dx.doi.org/10.1083/jcb.201610065 |
_version_ | 1783242272388677632 |
---|---|
author | Yamada, Moé Tanaka-Takiguchi, Yohko Hayashi, Masahito Nishina, Momoko Goshima, Gohta |
author_facet | Yamada, Moé Tanaka-Takiguchi, Yohko Hayashi, Masahito Nishina, Momoko Goshima, Gohta |
author_sort | Yamada, Moé |
collection | PubMed |
description | Minus end–directed cargo transport along microtubules (MTs) is exclusively driven by the molecular motor dynein in a wide variety of cell types. Interestingly, during evolution, plants have lost the genes encoding dynein; the MT motors that compensate for dynein function are unknown. Here, we show that two members of the kinesin-14 family drive minus end–directed transport in plants. Gene knockout analyses of the moss Physcomitrella patens revealed that the plant-specific class VI kinesin-14, KCBP, is required for minus end–directed transport of the nucleus and chloroplasts. Purified KCBP directly bound to acidic phospholipids and unidirectionally transported phospholipid liposomes along MTs in vitro. Thus, minus end–directed transport of membranous cargoes might be driven by their direct interaction with this motor protein. Newly nucleated cytoplasmic MTs represent another known cargo exhibiting minus end–directed motility, and we identified the conserved class I kinesin-14 (ATK) as the motor involved. These results suggest that kinesin-14 motors were duplicated and developed as alternative MT-based minus end–directed transporters in land plants. |
format | Online Article Text |
id | pubmed-5461021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54610212017-12-05 Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells Yamada, Moé Tanaka-Takiguchi, Yohko Hayashi, Masahito Nishina, Momoko Goshima, Gohta J Cell Biol Research Articles Minus end–directed cargo transport along microtubules (MTs) is exclusively driven by the molecular motor dynein in a wide variety of cell types. Interestingly, during evolution, plants have lost the genes encoding dynein; the MT motors that compensate for dynein function are unknown. Here, we show that two members of the kinesin-14 family drive minus end–directed transport in plants. Gene knockout analyses of the moss Physcomitrella patens revealed that the plant-specific class VI kinesin-14, KCBP, is required for minus end–directed transport of the nucleus and chloroplasts. Purified KCBP directly bound to acidic phospholipids and unidirectionally transported phospholipid liposomes along MTs in vitro. Thus, minus end–directed transport of membranous cargoes might be driven by their direct interaction with this motor protein. Newly nucleated cytoplasmic MTs represent another known cargo exhibiting minus end–directed motility, and we identified the conserved class I kinesin-14 (ATK) as the motor involved. These results suggest that kinesin-14 motors were duplicated and developed as alternative MT-based minus end–directed transporters in land plants. The Rockefeller University Press 2017-06-05 /pmc/articles/PMC5461021/ /pubmed/28442535 http://dx.doi.org/10.1083/jcb.201610065 Text en © 2017 Yamada et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Yamada, Moé Tanaka-Takiguchi, Yohko Hayashi, Masahito Nishina, Momoko Goshima, Gohta Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells |
title | Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells |
title_full | Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells |
title_fullStr | Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells |
title_full_unstemmed | Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells |
title_short | Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells |
title_sort | multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461021/ https://www.ncbi.nlm.nih.gov/pubmed/28442535 http://dx.doi.org/10.1083/jcb.201610065 |
work_keys_str_mv | AT yamadamoe multiplekinesin14familymembersdrivemicrotubuleminusenddirectedtransportinplantcells AT tanakatakiguchiyohko multiplekinesin14familymembersdrivemicrotubuleminusenddirectedtransportinplantcells AT hayashimasahito multiplekinesin14familymembersdrivemicrotubuleminusenddirectedtransportinplantcells AT nishinamomoko multiplekinesin14familymembersdrivemicrotubuleminusenddirectedtransportinplantcells AT goshimagohta multiplekinesin14familymembersdrivemicrotubuleminusenddirectedtransportinplantcells |