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Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells

Minus end–directed cargo transport along microtubules (MTs) is exclusively driven by the molecular motor dynein in a wide variety of cell types. Interestingly, during evolution, plants have lost the genes encoding dynein; the MT motors that compensate for dynein function are unknown. Here, we show t...

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Autores principales: Yamada, Moé, Tanaka-Takiguchi, Yohko, Hayashi, Masahito, Nishina, Momoko, Goshima, Gohta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461021/
https://www.ncbi.nlm.nih.gov/pubmed/28442535
http://dx.doi.org/10.1083/jcb.201610065
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author Yamada, Moé
Tanaka-Takiguchi, Yohko
Hayashi, Masahito
Nishina, Momoko
Goshima, Gohta
author_facet Yamada, Moé
Tanaka-Takiguchi, Yohko
Hayashi, Masahito
Nishina, Momoko
Goshima, Gohta
author_sort Yamada, Moé
collection PubMed
description Minus end–directed cargo transport along microtubules (MTs) is exclusively driven by the molecular motor dynein in a wide variety of cell types. Interestingly, during evolution, plants have lost the genes encoding dynein; the MT motors that compensate for dynein function are unknown. Here, we show that two members of the kinesin-14 family drive minus end–directed transport in plants. Gene knockout analyses of the moss Physcomitrella patens revealed that the plant-specific class VI kinesin-14, KCBP, is required for minus end–directed transport of the nucleus and chloroplasts. Purified KCBP directly bound to acidic phospholipids and unidirectionally transported phospholipid liposomes along MTs in vitro. Thus, minus end–directed transport of membranous cargoes might be driven by their direct interaction with this motor protein. Newly nucleated cytoplasmic MTs represent another known cargo exhibiting minus end–directed motility, and we identified the conserved class I kinesin-14 (ATK) as the motor involved. These results suggest that kinesin-14 motors were duplicated and developed as alternative MT-based minus end–directed transporters in land plants.
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spelling pubmed-54610212017-12-05 Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells Yamada, Moé Tanaka-Takiguchi, Yohko Hayashi, Masahito Nishina, Momoko Goshima, Gohta J Cell Biol Research Articles Minus end–directed cargo transport along microtubules (MTs) is exclusively driven by the molecular motor dynein in a wide variety of cell types. Interestingly, during evolution, plants have lost the genes encoding dynein; the MT motors that compensate for dynein function are unknown. Here, we show that two members of the kinesin-14 family drive minus end–directed transport in plants. Gene knockout analyses of the moss Physcomitrella patens revealed that the plant-specific class VI kinesin-14, KCBP, is required for minus end–directed transport of the nucleus and chloroplasts. Purified KCBP directly bound to acidic phospholipids and unidirectionally transported phospholipid liposomes along MTs in vitro. Thus, minus end–directed transport of membranous cargoes might be driven by their direct interaction with this motor protein. Newly nucleated cytoplasmic MTs represent another known cargo exhibiting minus end–directed motility, and we identified the conserved class I kinesin-14 (ATK) as the motor involved. These results suggest that kinesin-14 motors were duplicated and developed as alternative MT-based minus end–directed transporters in land plants. The Rockefeller University Press 2017-06-05 /pmc/articles/PMC5461021/ /pubmed/28442535 http://dx.doi.org/10.1083/jcb.201610065 Text en © 2017 Yamada et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Yamada, Moé
Tanaka-Takiguchi, Yohko
Hayashi, Masahito
Nishina, Momoko
Goshima, Gohta
Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells
title Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells
title_full Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells
title_fullStr Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells
title_full_unstemmed Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells
title_short Multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells
title_sort multiple kinesin-14 family members drive microtubule minus end–directed transport in plant cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461021/
https://www.ncbi.nlm.nih.gov/pubmed/28442535
http://dx.doi.org/10.1083/jcb.201610065
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