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Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe
Quantum dots (QDs) are semiconductor nanoparticles with a diameter of less than 10 nm, which have been widely used as fluorescent probes in biochemical analysis and vivo imaging because of their excellent optical properties. Sensitive and convenient detection of hepatitis B virus (HBV) gene mutation...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461085/ https://www.ncbi.nlm.nih.gov/pubmed/28445403 http://dx.doi.org/10.3390/s17050961 |
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author | Zhang, Cheng Chen, Yiping Liang, Xinmiao Zhang, Guanhua Ma, Hong Nie, Leng Wang, Yu |
author_facet | Zhang, Cheng Chen, Yiping Liang, Xinmiao Zhang, Guanhua Ma, Hong Nie, Leng Wang, Yu |
author_sort | Zhang, Cheng |
collection | PubMed |
description | Quantum dots (QDs) are semiconductor nanoparticles with a diameter of less than 10 nm, which have been widely used as fluorescent probes in biochemical analysis and vivo imaging because of their excellent optical properties. Sensitive and convenient detection of hepatitis B virus (HBV) gene mutations is important in clinical diagnosis. Therefore, we developed a sensitive, low-cost and convenient QDs-mediated fluorescent method for the detection of HBV gene mutations in real serum samples from chronic hepatitis B (CHB) patients who had received lamivudine or telbivudine antiviral therapy. We also evaluated the efficiency of this method for the detection of drug-resistant mutations compared with direct sequencing. In CHB, HBV DNA from the serum samples of patients with poor response or virological breakthrough can be hybridized to probes containing the M204I mutation to visualize fluorescence under fluorescence microscopy, where fluorescence intensity is related to the virus load, in our method. At present, the limits of the method used to detect HBV genetic variations by fluorescence quantum dots is 10(3) IU/mL. These results show that QDs can be used as fluorescent probes to detect viral HBV DNA polymerase gene variation, and is a simple readout system without complex and expensive instruments, which provides an attractive platform for the detection of HBV M204I mutation. |
format | Online Article Text |
id | pubmed-5461085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54610852017-06-16 Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe Zhang, Cheng Chen, Yiping Liang, Xinmiao Zhang, Guanhua Ma, Hong Nie, Leng Wang, Yu Sensors (Basel) Article Quantum dots (QDs) are semiconductor nanoparticles with a diameter of less than 10 nm, which have been widely used as fluorescent probes in biochemical analysis and vivo imaging because of their excellent optical properties. Sensitive and convenient detection of hepatitis B virus (HBV) gene mutations is important in clinical diagnosis. Therefore, we developed a sensitive, low-cost and convenient QDs-mediated fluorescent method for the detection of HBV gene mutations in real serum samples from chronic hepatitis B (CHB) patients who had received lamivudine or telbivudine antiviral therapy. We also evaluated the efficiency of this method for the detection of drug-resistant mutations compared with direct sequencing. In CHB, HBV DNA from the serum samples of patients with poor response or virological breakthrough can be hybridized to probes containing the M204I mutation to visualize fluorescence under fluorescence microscopy, where fluorescence intensity is related to the virus load, in our method. At present, the limits of the method used to detect HBV genetic variations by fluorescence quantum dots is 10(3) IU/mL. These results show that QDs can be used as fluorescent probes to detect viral HBV DNA polymerase gene variation, and is a simple readout system without complex and expensive instruments, which provides an attractive platform for the detection of HBV M204I mutation. MDPI 2017-04-26 /pmc/articles/PMC5461085/ /pubmed/28445403 http://dx.doi.org/10.3390/s17050961 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Cheng Chen, Yiping Liang, Xinmiao Zhang, Guanhua Ma, Hong Nie, Leng Wang, Yu Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe |
title | Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe |
title_full | Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe |
title_fullStr | Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe |
title_full_unstemmed | Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe |
title_short | Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe |
title_sort | detection of hepatitis b virus m204i mutation by quantum dot-labeled dna probe |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461085/ https://www.ncbi.nlm.nih.gov/pubmed/28445403 http://dx.doi.org/10.3390/s17050961 |
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