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An investigation on non-invasive fungal sinusitis; Molecular identification of etiologic agents
BACKGROUND: Fungal sinusitis is increasing worldwide in the past two decades. It is divided into two types including invasive and noninvasive. Noninvasive types contain allergic fungal sinusitis (AFS) and fungus ball. AFS is a hypersensitivity reaction to fungal allergens in the mucosa of the sinona...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461590/ https://www.ncbi.nlm.nih.gov/pubmed/28616054 http://dx.doi.org/10.4103/jrms.JRMS_166_17 |
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author | Mohammadi, Abdolrasoul Hashemi, Seyed Mostafa Abtahi, Sayed Hamidreza Lajevardi, Seyed Mohammad Kianipour, Sahar Mohammadi, Rasoul |
author_facet | Mohammadi, Abdolrasoul Hashemi, Seyed Mostafa Abtahi, Sayed Hamidreza Lajevardi, Seyed Mohammad Kianipour, Sahar Mohammadi, Rasoul |
author_sort | Mohammadi, Abdolrasoul |
collection | PubMed |
description | BACKGROUND: Fungal sinusitis is increasing worldwide in the past two decades. It is divided into two types including invasive and noninvasive. Noninvasive types contain allergic fungal sinusitis (AFS) and fungus ball. AFS is a hypersensitivity reaction to fungal allergens in the mucosa of the sinonasal tract in atopic individuals. The fungus ball is a different type of noninvasive fungal rhinosinusitis which is delineated as an accumulation of debris and fungal elements inside a paranasal sinus. Fungal sinusitis caused by various fungi such as Aspergillus species, Penicillium, Mucor, Rhizopus, and phaeohyphomycetes. The aim of the present study is to identify fungal species isolated from noninvasive fungal sinusitis by molecular methods. MATERIALS AND METHODS: During 2015–2016, a total of 100 suspected patients were examined for fungal sinusitis. Functional endoscopic sinus surgery was performed using the Messerklinger technique. Clinical samples were identified by phenotypic and molecular methods. Polymerase chain reaction (PCR) sequencing of ITS1-5.8S-ITS2 region and PCR-restriction fragment length polymorphism with MspI restriction enzyme was performed for molecular identification of molds and yeasts, respectively. RESULTS: Twenty-seven out of 100 suspected cases (27%) had fungal sinusitis. Nasal congestion (59%) and headache (19%) were the most common clinical signs among patients. Fifteen patients (55.5%) were male and 12 patients (44.5%) were female. Aspergillus flavus was the most prevalent fungal species (26%), followed by Penicillium chrysogenum (18.5%) and Candida glabrata species complex (15%). CONCLUSION: Since clinical manifestations, computed tomography scan, endoscopy, and histopathological findings are very nonspecific in AFS and fungus ball; therefore, molecular investigations are compulsory for precise identification of etiologic agents and appropriate management of these fungal infections. |
format | Online Article Text |
id | pubmed-5461590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-54615902017-06-14 An investigation on non-invasive fungal sinusitis; Molecular identification of etiologic agents Mohammadi, Abdolrasoul Hashemi, Seyed Mostafa Abtahi, Sayed Hamidreza Lajevardi, Seyed Mohammad Kianipour, Sahar Mohammadi, Rasoul J Res Med Sci Original Article BACKGROUND: Fungal sinusitis is increasing worldwide in the past two decades. It is divided into two types including invasive and noninvasive. Noninvasive types contain allergic fungal sinusitis (AFS) and fungus ball. AFS is a hypersensitivity reaction to fungal allergens in the mucosa of the sinonasal tract in atopic individuals. The fungus ball is a different type of noninvasive fungal rhinosinusitis which is delineated as an accumulation of debris and fungal elements inside a paranasal sinus. Fungal sinusitis caused by various fungi such as Aspergillus species, Penicillium, Mucor, Rhizopus, and phaeohyphomycetes. The aim of the present study is to identify fungal species isolated from noninvasive fungal sinusitis by molecular methods. MATERIALS AND METHODS: During 2015–2016, a total of 100 suspected patients were examined for fungal sinusitis. Functional endoscopic sinus surgery was performed using the Messerklinger technique. Clinical samples were identified by phenotypic and molecular methods. Polymerase chain reaction (PCR) sequencing of ITS1-5.8S-ITS2 region and PCR-restriction fragment length polymorphism with MspI restriction enzyme was performed for molecular identification of molds and yeasts, respectively. RESULTS: Twenty-seven out of 100 suspected cases (27%) had fungal sinusitis. Nasal congestion (59%) and headache (19%) were the most common clinical signs among patients. Fifteen patients (55.5%) were male and 12 patients (44.5%) were female. Aspergillus flavus was the most prevalent fungal species (26%), followed by Penicillium chrysogenum (18.5%) and Candida glabrata species complex (15%). CONCLUSION: Since clinical manifestations, computed tomography scan, endoscopy, and histopathological findings are very nonspecific in AFS and fungus ball; therefore, molecular investigations are compulsory for precise identification of etiologic agents and appropriate management of these fungal infections. Medknow Publications & Media Pvt Ltd 2017-05-30 /pmc/articles/PMC5461590/ /pubmed/28616054 http://dx.doi.org/10.4103/jrms.JRMS_166_17 Text en Copyright: © 2017 Journal of Research in Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Mohammadi, Abdolrasoul Hashemi, Seyed Mostafa Abtahi, Sayed Hamidreza Lajevardi, Seyed Mohammad Kianipour, Sahar Mohammadi, Rasoul An investigation on non-invasive fungal sinusitis; Molecular identification of etiologic agents |
title | An investigation on non-invasive fungal sinusitis; Molecular identification of etiologic agents |
title_full | An investigation on non-invasive fungal sinusitis; Molecular identification of etiologic agents |
title_fullStr | An investigation on non-invasive fungal sinusitis; Molecular identification of etiologic agents |
title_full_unstemmed | An investigation on non-invasive fungal sinusitis; Molecular identification of etiologic agents |
title_short | An investigation on non-invasive fungal sinusitis; Molecular identification of etiologic agents |
title_sort | investigation on non-invasive fungal sinusitis; molecular identification of etiologic agents |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461590/ https://www.ncbi.nlm.nih.gov/pubmed/28616054 http://dx.doi.org/10.4103/jrms.JRMS_166_17 |
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