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A systematic evaluation of nucleotide properties for CRISPR sgRNA design

BACKGROUND: CRISPR is a versatile gene editing tool which has revolutionized genetic research in the past few years. Optimizing sgRNA design to improve the efficiency of target/DNA cleavage is critical to ensure the success of CRISPR screens. RESULTS: By borrowing knowledge from oligonucleotide desi...

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Detalles Bibliográficos
Autores principales:	Kuan, Pei Fen, Powers, Scott, He, Shuyao, Li, Kaiqiao, Zhao, Xiaoyu, Huang, Bo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461693/ https://www.ncbi.nlm.nih.gov/pubmed/28587596 http://dx.doi.org/10.1186/s12859-017-1697-6

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