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Effects of topical insulin on second-intention wound healing in the red-eared slider turtle (Trachemys scripta elegans) – a controlled study

BACKGROUND: Compared with mammals, wound healing in reptiles is characterized by reduced wound contraction and longer healing times. The aim of this study is to describe the clinical and histopathological effects of topical insulin on second-intention healing of experimentally induced wounds in skin...

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Autores principales: Negrini, Joao, Mozos, Elena, Escamilla, Alejandro, Pérez, José, Lucena, Rosario, Guerra, Rafael, Ginel, Pedro J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461716/
https://www.ncbi.nlm.nih.gov/pubmed/28587643
http://dx.doi.org/10.1186/s12917-017-1082-8
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author Negrini, Joao
Mozos, Elena
Escamilla, Alejandro
Pérez, José
Lucena, Rosario
Guerra, Rafael
Ginel, Pedro J.
author_facet Negrini, Joao
Mozos, Elena
Escamilla, Alejandro
Pérez, José
Lucena, Rosario
Guerra, Rafael
Ginel, Pedro J.
author_sort Negrini, Joao
collection PubMed
description BACKGROUND: Compared with mammals, wound healing in reptiles is characterized by reduced wound contraction and longer healing times. The aim of this study is to describe the clinical and histopathological effects of topical insulin on second-intention healing of experimentally induced wounds in skin without dermal bony plates of Trachemys scripta elegans exposed to daily variations in ambient temperature and in an aquatic environment. Forty-four healthy adult females were assigned to two groups: Group 1 (n = 24) was used to assess clinical features such as wound contraction; Group 2 (n = 20) was used for histological evaluation and morphometric analysis. Topical porcine insulin (5 IU/ml diluted in glycerol) was applied daily 1 week. For each control time (2, 7, 14, 21 and 28 days post-wounding), re-epithelisation and wound remodelling were evaluated histologically and the number of main inflammatory cells (heterophils, macrophages, lymphocytes and fibroblasts) was scored. RESULTS: Mean wound contraction was higher in the insulin-treated group at each time point and differences were significant at day 28 (P < 0.0001). Histologically, these clinical findings were associated with better re-epithelisation, inflammatory response, collagen synthesis and remodelling of the wounds. Morphometrically, insulin-treated wounds had significantly higher mean counts of heterophils (day 7), macrophages (days 2, 7 and 14) and fibroblasts (days 14 and 21), whereas lymphocyte counts were significantly lower at day 21. These results demonstrate that topical insulin modifies the inflammatory response of turtle skin up-regulating inflammatory cells at early stages and promoting wound healing. CONCLUSIONS: Topical insulin is a potentially useful therapy in skin wounds of Trachemys scripta and should be evaluated in non-experimental wounds of turtles and other reptiles.
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spelling pubmed-54617162017-06-07 Effects of topical insulin on second-intention wound healing in the red-eared slider turtle (Trachemys scripta elegans) – a controlled study Negrini, Joao Mozos, Elena Escamilla, Alejandro Pérez, José Lucena, Rosario Guerra, Rafael Ginel, Pedro J. BMC Vet Res Research Article BACKGROUND: Compared with mammals, wound healing in reptiles is characterized by reduced wound contraction and longer healing times. The aim of this study is to describe the clinical and histopathological effects of topical insulin on second-intention healing of experimentally induced wounds in skin without dermal bony plates of Trachemys scripta elegans exposed to daily variations in ambient temperature and in an aquatic environment. Forty-four healthy adult females were assigned to two groups: Group 1 (n = 24) was used to assess clinical features such as wound contraction; Group 2 (n = 20) was used for histological evaluation and morphometric analysis. Topical porcine insulin (5 IU/ml diluted in glycerol) was applied daily 1 week. For each control time (2, 7, 14, 21 and 28 days post-wounding), re-epithelisation and wound remodelling were evaluated histologically and the number of main inflammatory cells (heterophils, macrophages, lymphocytes and fibroblasts) was scored. RESULTS: Mean wound contraction was higher in the insulin-treated group at each time point and differences were significant at day 28 (P < 0.0001). Histologically, these clinical findings were associated with better re-epithelisation, inflammatory response, collagen synthesis and remodelling of the wounds. Morphometrically, insulin-treated wounds had significantly higher mean counts of heterophils (day 7), macrophages (days 2, 7 and 14) and fibroblasts (days 14 and 21), whereas lymphocyte counts were significantly lower at day 21. These results demonstrate that topical insulin modifies the inflammatory response of turtle skin up-regulating inflammatory cells at early stages and promoting wound healing. CONCLUSIONS: Topical insulin is a potentially useful therapy in skin wounds of Trachemys scripta and should be evaluated in non-experimental wounds of turtles and other reptiles. BioMed Central 2017-06-06 /pmc/articles/PMC5461716/ /pubmed/28587643 http://dx.doi.org/10.1186/s12917-017-1082-8 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Negrini, Joao
Mozos, Elena
Escamilla, Alejandro
Pérez, José
Lucena, Rosario
Guerra, Rafael
Ginel, Pedro J.
Effects of topical insulin on second-intention wound healing in the red-eared slider turtle (Trachemys scripta elegans) – a controlled study
title Effects of topical insulin on second-intention wound healing in the red-eared slider turtle (Trachemys scripta elegans) – a controlled study
title_full Effects of topical insulin on second-intention wound healing in the red-eared slider turtle (Trachemys scripta elegans) – a controlled study
title_fullStr Effects of topical insulin on second-intention wound healing in the red-eared slider turtle (Trachemys scripta elegans) – a controlled study
title_full_unstemmed Effects of topical insulin on second-intention wound healing in the red-eared slider turtle (Trachemys scripta elegans) – a controlled study
title_short Effects of topical insulin on second-intention wound healing in the red-eared slider turtle (Trachemys scripta elegans) – a controlled study
title_sort effects of topical insulin on second-intention wound healing in the red-eared slider turtle (trachemys scripta elegans) – a controlled study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461716/
https://www.ncbi.nlm.nih.gov/pubmed/28587643
http://dx.doi.org/10.1186/s12917-017-1082-8
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