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Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination
BACKGROUND: Sinapic acid and its esters have broad functions in different stages of seed germination and plant development and are thought to play a role in protecting against ultraviolet irradiation. To better understand the interactions between sinapic acid esters and seed germination processes in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461752/ https://www.ncbi.nlm.nih.gov/pubmed/28587634 http://dx.doi.org/10.1186/s12870-017-1048-9 |
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author | Bi, Baodi Tang, Jingliang Han, Shuang Guo, Jinggong Miao, Yuchen |
author_facet | Bi, Baodi Tang, Jingliang Han, Shuang Guo, Jinggong Miao, Yuchen |
author_sort | Bi, Baodi |
collection | PubMed |
description | BACKGROUND: Sinapic acid and its esters have broad functions in different stages of seed germination and plant development and are thought to play a role in protecting against ultraviolet irradiation. To better understand the interactions between sinapic acid esters and seed germination processes in response to various stresses, we analyzed the role of the plant hormone abscisic acid (ABA) in the regulation of sinapic acid esters involved in seed germination and early seedling growth. RESULTS: We found that exogenous sinapic acid promotes seed germination in a dose-dependent manner in Arabidopsis thaliana. High-performance liquid chromatography mass spectrometry analysis showed that exogenous sinapic acid increased the sinapoylcholine content of imbibed seeds. Furthermore, sinapic acid affected ABA catabolism, resulting in reduced ABA levels and increased levels of the ABA-glucose ester. Using mutants deficient in the synthesis of sinapate esters, we showed that the germination of mutant sinapoylglucose accumulator 2 (sng2) and bright trichomes 1 (brt1) seeds was more sensitive to ABA than the wild-type. Moreover, Arabidopsis mutants deficient in either abscisic acid deficient 2 (ABA2) or abscisic acid insensitive 3 (ABI3) displayed increased expression of the sinapoylglucose:choline sinapoyltransferase (SCT) and sinapoylcholine esterase (SCE) genes with sinapic acid treatment. This treatment also affected the accumulation of sinapoylcholine and free choline during seed germination. CONCLUSIONS: We demonstrated that sinapoylcholine, which constitutes the major phenolic component in seeds among various minor sinapate esters, affected ABA homeostasis during seed germination and early seedling growth in Arabidopsis. Our findings provide insights into the role of sinapic acid and its esters in regulating ABA-mediated inhibition of Arabidopsis seed germination in response to drought stress. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-017-1048-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5461752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54617522017-06-07 Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination Bi, Baodi Tang, Jingliang Han, Shuang Guo, Jinggong Miao, Yuchen BMC Plant Biol Research Article BACKGROUND: Sinapic acid and its esters have broad functions in different stages of seed germination and plant development and are thought to play a role in protecting against ultraviolet irradiation. To better understand the interactions between sinapic acid esters and seed germination processes in response to various stresses, we analyzed the role of the plant hormone abscisic acid (ABA) in the regulation of sinapic acid esters involved in seed germination and early seedling growth. RESULTS: We found that exogenous sinapic acid promotes seed germination in a dose-dependent manner in Arabidopsis thaliana. High-performance liquid chromatography mass spectrometry analysis showed that exogenous sinapic acid increased the sinapoylcholine content of imbibed seeds. Furthermore, sinapic acid affected ABA catabolism, resulting in reduced ABA levels and increased levels of the ABA-glucose ester. Using mutants deficient in the synthesis of sinapate esters, we showed that the germination of mutant sinapoylglucose accumulator 2 (sng2) and bright trichomes 1 (brt1) seeds was more sensitive to ABA than the wild-type. Moreover, Arabidopsis mutants deficient in either abscisic acid deficient 2 (ABA2) or abscisic acid insensitive 3 (ABI3) displayed increased expression of the sinapoylglucose:choline sinapoyltransferase (SCT) and sinapoylcholine esterase (SCE) genes with sinapic acid treatment. This treatment also affected the accumulation of sinapoylcholine and free choline during seed germination. CONCLUSIONS: We demonstrated that sinapoylcholine, which constitutes the major phenolic component in seeds among various minor sinapate esters, affected ABA homeostasis during seed germination and early seedling growth in Arabidopsis. Our findings provide insights into the role of sinapic acid and its esters in regulating ABA-mediated inhibition of Arabidopsis seed germination in response to drought stress. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-017-1048-9) contains supplementary material, which is available to authorized users. BioMed Central 2017-06-06 /pmc/articles/PMC5461752/ /pubmed/28587634 http://dx.doi.org/10.1186/s12870-017-1048-9 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Bi, Baodi Tang, Jingliang Han, Shuang Guo, Jinggong Miao, Yuchen Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination |
title | Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination |
title_full | Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination |
title_fullStr | Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination |
title_full_unstemmed | Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination |
title_short | Sinapic acid or its derivatives interfere with abscisic acid homeostasis during Arabidopsis thaliana seed germination |
title_sort | sinapic acid or its derivatives interfere with abscisic acid homeostasis during arabidopsis thaliana seed germination |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5461752/ https://www.ncbi.nlm.nih.gov/pubmed/28587634 http://dx.doi.org/10.1186/s12870-017-1048-9 |
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