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Urinary excretion of EGF and MCP-1 in children with vesico-ureteral reflux

PURPOSE: The aim of this study was to investigate the urinary concentration of epidermal growth factor (EGF) and monocyte chemotactic protein-1 (MCP-1) as reflux nephropathy (RN) biomarkers before and after endoscopic treatment of moderate to severe vesico-ureteral reflux (VUR). MATERIALS AND METHOD...

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Autores principales: Pastore, Valentina, Bartoli, Fabio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Urologia 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462148/
https://www.ncbi.nlm.nih.gov/pubmed/28191787
http://dx.doi.org/10.1590/S1677-5538.IBJU.2015.0132
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author Pastore, Valentina
Bartoli, Fabio
author_facet Pastore, Valentina
Bartoli, Fabio
author_sort Pastore, Valentina
collection PubMed
description PURPOSE: The aim of this study was to investigate the urinary concentration of epidermal growth factor (EGF) and monocyte chemotactic protein-1 (MCP-1) as reflux nephropathy (RN) biomarkers before and after endoscopic treatment of moderate to severe vesico-ureteral reflux (VUR). MATERIALS AND METHODS: A prospective study was carried out on 72 children with moderate to severe VUR. All patients underwent endoscopic treatment using Macroplastique® or Deflux®. Vesico-ureteral reflux resolution was tested by post-operative voiding cystourethrography after 3 months and 2 years. Follow-up urinary samples were collected at that time. Control samples were taken from healthy children with no clinical evidence of renal and bladder disease and no history of UTI. RESULTS: In VUR patients, pre-operative urinary EGF levels had a down-regulation when compared to controls. Following successful VUR repair, urinary EGF levels of VUR children progressively increased only at long term follow-up but without returning to normal levels. Urinary MCP-1 levels were highly expressed in pre-operative samples and decreased markedly during early post-operative measurements. Urinary MCP-1 levels did not further decreased in late post-operative follow-up. In fact, these levels remained significantly higher when compared to controls. CONCLUSIONS: Urinary levels of EGF and MCP-1 may become useful markers for monitoring the response to surgical treatment in VUR patients. Although endoscopic VUR treatment is effective in reducing the inflammatory response, the persistence of significant abnormal levels of inflammatory cytokines (such as urinary MCP-1) at long term follow-up suggests that surgery alone may not completely treat the chronic renal inflammation evidenced in these children.
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spelling pubmed-54621482017-06-19 Urinary excretion of EGF and MCP-1 in children with vesico-ureteral reflux Pastore, Valentina Bartoli, Fabio Int Braz J Urol Original Article PURPOSE: The aim of this study was to investigate the urinary concentration of epidermal growth factor (EGF) and monocyte chemotactic protein-1 (MCP-1) as reflux nephropathy (RN) biomarkers before and after endoscopic treatment of moderate to severe vesico-ureteral reflux (VUR). MATERIALS AND METHODS: A prospective study was carried out on 72 children with moderate to severe VUR. All patients underwent endoscopic treatment using Macroplastique® or Deflux®. Vesico-ureteral reflux resolution was tested by post-operative voiding cystourethrography after 3 months and 2 years. Follow-up urinary samples were collected at that time. Control samples were taken from healthy children with no clinical evidence of renal and bladder disease and no history of UTI. RESULTS: In VUR patients, pre-operative urinary EGF levels had a down-regulation when compared to controls. Following successful VUR repair, urinary EGF levels of VUR children progressively increased only at long term follow-up but without returning to normal levels. Urinary MCP-1 levels were highly expressed in pre-operative samples and decreased markedly during early post-operative measurements. Urinary MCP-1 levels did not further decreased in late post-operative follow-up. In fact, these levels remained significantly higher when compared to controls. CONCLUSIONS: Urinary levels of EGF and MCP-1 may become useful markers for monitoring the response to surgical treatment in VUR patients. Although endoscopic VUR treatment is effective in reducing the inflammatory response, the persistence of significant abnormal levels of inflammatory cytokines (such as urinary MCP-1) at long term follow-up suggests that surgery alone may not completely treat the chronic renal inflammation evidenced in these children. Sociedade Brasileira de Urologia 2017 /pmc/articles/PMC5462148/ /pubmed/28191787 http://dx.doi.org/10.1590/S1677-5538.IBJU.2015.0132 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Pastore, Valentina
Bartoli, Fabio
Urinary excretion of EGF and MCP-1 in children with vesico-ureteral reflux
title Urinary excretion of EGF and MCP-1 in children with vesico-ureteral reflux
title_full Urinary excretion of EGF and MCP-1 in children with vesico-ureteral reflux
title_fullStr Urinary excretion of EGF and MCP-1 in children with vesico-ureteral reflux
title_full_unstemmed Urinary excretion of EGF and MCP-1 in children with vesico-ureteral reflux
title_short Urinary excretion of EGF and MCP-1 in children with vesico-ureteral reflux
title_sort urinary excretion of egf and mcp-1 in children with vesico-ureteral reflux
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462148/
https://www.ncbi.nlm.nih.gov/pubmed/28191787
http://dx.doi.org/10.1590/S1677-5538.IBJU.2015.0132
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