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Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases
BACKGROUND: Studies have found that c-Abl oncogenic kinases may regulate gene transcription by RNA polymerase II phosphorylation or by direct regulation of specific transcription factors or coactivators. However, the global regulation of differential gene expression by c-Abl/Arg is largely unknown....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462482/ https://www.ncbi.nlm.nih.gov/pubmed/28555614 http://dx.doi.org/10.12659/MSM.904888 |
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author | Dong, Qincai Li, Chenggong Qu, Xiuhua Cao, Cheng Liu, Xuan |
author_facet | Dong, Qincai Li, Chenggong Qu, Xiuhua Cao, Cheng Liu, Xuan |
author_sort | Dong, Qincai |
collection | PubMed |
description | BACKGROUND: Studies have found that c-Abl oncogenic kinases may regulate gene transcription by RNA polymerase II phosphorylation or by direct regulation of specific transcription factors or coactivators. However, the global regulation of differential gene expression by c-Abl/Arg is largely unknown. In this study, differentially expressed genes (DEGs) regulated by c-Abl/Arg were identified, and related cellular functions and associated pathways were investigated. MATERIAL/METHODS: RNA obtained from wild-type and c-Abl/Arg gene-silenced MCF-7 cells was analyzed by RNA-Seq. DEGs were identified using edgeR software and partially validated by qRT-PCR. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to explore the potential functions of these DEGs. RESULTS: A total of 1,034 DEGs were significantly regulated by c-Abl/Arg (399 were up-regulated and 635 were down-regulated after c-Abl/Arg double knockdown). GO and KEGG analyses showed that the DEGs were primarily involved in cellular metabolic processes, neurodegenerative disease, the metabolic process and signaling pathway of cAMP, angiogenesis, and cell proliferation. CONCLUSIONS: Our data collectively support the hypothesis that c-Abl/Arg regulate differential gene expression, providing new insights into the biological functions of c-Abl and Arg. |
format | Online Article Text |
id | pubmed-5462482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54624822017-06-14 Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases Dong, Qincai Li, Chenggong Qu, Xiuhua Cao, Cheng Liu, Xuan Med Sci Monit Lab/In Vitro Research BACKGROUND: Studies have found that c-Abl oncogenic kinases may regulate gene transcription by RNA polymerase II phosphorylation or by direct regulation of specific transcription factors or coactivators. However, the global regulation of differential gene expression by c-Abl/Arg is largely unknown. In this study, differentially expressed genes (DEGs) regulated by c-Abl/Arg were identified, and related cellular functions and associated pathways were investigated. MATERIAL/METHODS: RNA obtained from wild-type and c-Abl/Arg gene-silenced MCF-7 cells was analyzed by RNA-Seq. DEGs were identified using edgeR software and partially validated by qRT-PCR. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to explore the potential functions of these DEGs. RESULTS: A total of 1,034 DEGs were significantly regulated by c-Abl/Arg (399 were up-regulated and 635 were down-regulated after c-Abl/Arg double knockdown). GO and KEGG analyses showed that the DEGs were primarily involved in cellular metabolic processes, neurodegenerative disease, the metabolic process and signaling pathway of cAMP, angiogenesis, and cell proliferation. CONCLUSIONS: Our data collectively support the hypothesis that c-Abl/Arg regulate differential gene expression, providing new insights into the biological functions of c-Abl and Arg. International Scientific Literature, Inc. 2017-05-30 /pmc/articles/PMC5462482/ /pubmed/28555614 http://dx.doi.org/10.12659/MSM.904888 Text en © Med Sci Monit, 2017 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Lab/In Vitro Research Dong, Qincai Li, Chenggong Qu, Xiuhua Cao, Cheng Liu, Xuan Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases |
title | Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases |
title_full | Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases |
title_fullStr | Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases |
title_full_unstemmed | Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases |
title_short | Global Regulation of Differential Gene Expression by c-Abl/Arg Oncogenic Kinases |
title_sort | global regulation of differential gene expression by c-abl/arg oncogenic kinases |
topic | Lab/In Vitro Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462482/ https://www.ncbi.nlm.nih.gov/pubmed/28555614 http://dx.doi.org/10.12659/MSM.904888 |
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