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Overexpression of BdMATE Gene Improves Aluminum Tolerance in Setaria viridis
Acidic soils are distributed worldwide, predominantly in tropical and subtropical areas, reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiolog...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462932/ https://www.ncbi.nlm.nih.gov/pubmed/28642761 http://dx.doi.org/10.3389/fpls.2017.00865 |
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author | Ribeiro, Ana P. de Souza, Wagner R. Martins, Polyana K. Vinecky, Felipe Duarte, Karoline E. Basso, Marcos F. da Cunha, Bárbara A. D. B. Campanha, Raquel B. de Oliveira, Patrícia A. Centeno, Danilo C. Cançado, Geraldo M. A. de Magalhães, Jurandir V. de Sousa, Carlos A. F. Andrade, Alan C. Kobayashi, Adilson K. Molinari, Hugo B. C. |
author_facet | Ribeiro, Ana P. de Souza, Wagner R. Martins, Polyana K. Vinecky, Felipe Duarte, Karoline E. Basso, Marcos F. da Cunha, Bárbara A. D. B. Campanha, Raquel B. de Oliveira, Patrícia A. Centeno, Danilo C. Cançado, Geraldo M. A. de Magalhães, Jurandir V. de Sousa, Carlos A. F. Andrade, Alan C. Kobayashi, Adilson K. Molinari, Hugo B. C. |
author_sort | Ribeiro, Ana P. |
collection | PubMed |
description | Acidic soils are distributed worldwide, predominantly in tropical and subtropical areas, reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiological mechanism used by plants to cope with Al stress involves activation of membrane transporters responsible for organic acid anions secretion from the root apex to the rhizosphere, which chelate Al, preventing its absorption by roots. In sorghum, a membrane transporter gene belonging to multidrug and toxic compound extrusion (MATE) family was identified and characterized as an aluminum-activated citrate transporter gene responsible for Al tolerance in this crop. Setaria viridis is an emerging model for C4 species and it is an important model to validate some genes for further C4 crops transformation, such as sugarcane, maize, and wheat. In the present work, Setaria viridis was used as a model plant to overexpress a newly identified MATE gene from Brachypodium distachyon (BdMATE), closely related to SbMATE, for aluminum tolerance assays. Transgenic S. viridis plants overexpressing a BdMATE presented an improved Al tolerance phenotype, characterized by sustained root growth and exclusion of aluminum from the root apex in transgenic plants, as confirmed by hematoxylin assay. In addition, transgenic plants showed higher root citrate exudation into the rhizosphere, suggesting that Al tolerance improvement in these plants could be related to the chelation of the metal by the organic acid anion. These results suggest that BdMATE gene can be used to transform C4 crops of economic importance with improved aluminum tolerance. |
format | Online Article Text |
id | pubmed-5462932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54629322017-06-22 Overexpression of BdMATE Gene Improves Aluminum Tolerance in Setaria viridis Ribeiro, Ana P. de Souza, Wagner R. Martins, Polyana K. Vinecky, Felipe Duarte, Karoline E. Basso, Marcos F. da Cunha, Bárbara A. D. B. Campanha, Raquel B. de Oliveira, Patrícia A. Centeno, Danilo C. Cançado, Geraldo M. A. de Magalhães, Jurandir V. de Sousa, Carlos A. F. Andrade, Alan C. Kobayashi, Adilson K. Molinari, Hugo B. C. Front Plant Sci Plant Science Acidic soils are distributed worldwide, predominantly in tropical and subtropical areas, reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiological mechanism used by plants to cope with Al stress involves activation of membrane transporters responsible for organic acid anions secretion from the root apex to the rhizosphere, which chelate Al, preventing its absorption by roots. In sorghum, a membrane transporter gene belonging to multidrug and toxic compound extrusion (MATE) family was identified and characterized as an aluminum-activated citrate transporter gene responsible for Al tolerance in this crop. Setaria viridis is an emerging model for C4 species and it is an important model to validate some genes for further C4 crops transformation, such as sugarcane, maize, and wheat. In the present work, Setaria viridis was used as a model plant to overexpress a newly identified MATE gene from Brachypodium distachyon (BdMATE), closely related to SbMATE, for aluminum tolerance assays. Transgenic S. viridis plants overexpressing a BdMATE presented an improved Al tolerance phenotype, characterized by sustained root growth and exclusion of aluminum from the root apex in transgenic plants, as confirmed by hematoxylin assay. In addition, transgenic plants showed higher root citrate exudation into the rhizosphere, suggesting that Al tolerance improvement in these plants could be related to the chelation of the metal by the organic acid anion. These results suggest that BdMATE gene can be used to transform C4 crops of economic importance with improved aluminum tolerance. Frontiers Media S.A. 2017-06-08 /pmc/articles/PMC5462932/ /pubmed/28642761 http://dx.doi.org/10.3389/fpls.2017.00865 Text en Copyright © 2017 Ribeiro, de Souza, Martins, Vinecky, Duarte, Basso, da Cunha, Campanha, de Oliveira, Centeno, Cançado, de Magalhães, de Sousa, Andrade, Kobayashi and Molinari. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Ribeiro, Ana P. de Souza, Wagner R. Martins, Polyana K. Vinecky, Felipe Duarte, Karoline E. Basso, Marcos F. da Cunha, Bárbara A. D. B. Campanha, Raquel B. de Oliveira, Patrícia A. Centeno, Danilo C. Cançado, Geraldo M. A. de Magalhães, Jurandir V. de Sousa, Carlos A. F. Andrade, Alan C. Kobayashi, Adilson K. Molinari, Hugo B. C. Overexpression of BdMATE Gene Improves Aluminum Tolerance in Setaria viridis |
title | Overexpression of BdMATE Gene Improves Aluminum Tolerance in Setaria viridis |
title_full | Overexpression of BdMATE Gene Improves Aluminum Tolerance in Setaria viridis |
title_fullStr | Overexpression of BdMATE Gene Improves Aluminum Tolerance in Setaria viridis |
title_full_unstemmed | Overexpression of BdMATE Gene Improves Aluminum Tolerance in Setaria viridis |
title_short | Overexpression of BdMATE Gene Improves Aluminum Tolerance in Setaria viridis |
title_sort | overexpression of bdmate gene improves aluminum tolerance in setaria viridis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462932/ https://www.ncbi.nlm.nih.gov/pubmed/28642761 http://dx.doi.org/10.3389/fpls.2017.00865 |
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