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Unexpected Dominance of Elusive Acidobacteria in Early Industrial Soft Coal Slags

Acid mine drainage (AMD) and mine tailing environments are well-characterized ecosystems known to be dominated by organisms involved in iron- and sulfur-cycling. Here we examined the microbiology of industrial soft coal slags that originate from alum leaching, an ecosystem distantly related to AMD e...

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Autores principales: Wegner, Carl-Eric, Liesack, Werner
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462947/
https://www.ncbi.nlm.nih.gov/pubmed/28642744
http://dx.doi.org/10.3389/fmicb.2017.01023
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author Wegner, Carl-Eric
Liesack, Werner
author_facet Wegner, Carl-Eric
Liesack, Werner
author_sort Wegner, Carl-Eric
collection PubMed
description Acid mine drainage (AMD) and mine tailing environments are well-characterized ecosystems known to be dominated by organisms involved in iron- and sulfur-cycling. Here we examined the microbiology of industrial soft coal slags that originate from alum leaching, an ecosystem distantly related to AMD environments. Our study involved geochemical analyses, bacterial community profiling, and shotgun metagenomics. The slags still contained high amounts of alum constituents (aluminum, sulfur), which mediated direct and indirect effects on bacterial community structure. Bacterial groups typically found in AMD systems and mine tailings were not present. Instead, the soft coal slags were dominated by uncharacterized groups of Acidobacteria (DA052 [subdivision 2], KF-JG30-18 [subdivision 13]), Actinobacteria (TM214), Alphaproteobacteria (DA111), and Chloroflexi (JG37-AG-4), which have previously been detected primarily in peatlands and uranium waste piles. Shotgun metagenomics allowed us to reconstruct 13 high-quality Acidobacteria draft genomes, of which two genomes could be directly linked to dominating groups (DA052, KF-JG30-18) by recovered 16S rRNA gene sequences. Comparative genomics revealed broad carbon utilization capabilities for these two groups of elusive Acidobacteria, including polysaccharide breakdown (cellulose, xylan) and the competence to metabolize C1 compounds (ribulose monophosphate pathway) and lignin derivatives (dye-decolorizing peroxidases). Equipped with a broad range of efflux systems for metal cations and xenobiotics, DA052 and KF-JG30-18 may have a competitive advantage over other bacterial groups in this unique habitat.
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spelling pubmed-54629472017-06-22 Unexpected Dominance of Elusive Acidobacteria in Early Industrial Soft Coal Slags Wegner, Carl-Eric Liesack, Werner Front Microbiol Microbiology Acid mine drainage (AMD) and mine tailing environments are well-characterized ecosystems known to be dominated by organisms involved in iron- and sulfur-cycling. Here we examined the microbiology of industrial soft coal slags that originate from alum leaching, an ecosystem distantly related to AMD environments. Our study involved geochemical analyses, bacterial community profiling, and shotgun metagenomics. The slags still contained high amounts of alum constituents (aluminum, sulfur), which mediated direct and indirect effects on bacterial community structure. Bacterial groups typically found in AMD systems and mine tailings were not present. Instead, the soft coal slags were dominated by uncharacterized groups of Acidobacteria (DA052 [subdivision 2], KF-JG30-18 [subdivision 13]), Actinobacteria (TM214), Alphaproteobacteria (DA111), and Chloroflexi (JG37-AG-4), which have previously been detected primarily in peatlands and uranium waste piles. Shotgun metagenomics allowed us to reconstruct 13 high-quality Acidobacteria draft genomes, of which two genomes could be directly linked to dominating groups (DA052, KF-JG30-18) by recovered 16S rRNA gene sequences. Comparative genomics revealed broad carbon utilization capabilities for these two groups of elusive Acidobacteria, including polysaccharide breakdown (cellulose, xylan) and the competence to metabolize C1 compounds (ribulose monophosphate pathway) and lignin derivatives (dye-decolorizing peroxidases). Equipped with a broad range of efflux systems for metal cations and xenobiotics, DA052 and KF-JG30-18 may have a competitive advantage over other bacterial groups in this unique habitat. Frontiers Media S.A. 2017-06-08 /pmc/articles/PMC5462947/ /pubmed/28642744 http://dx.doi.org/10.3389/fmicb.2017.01023 Text en Copyright © 2017 Wegner and Liesack. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wegner, Carl-Eric
Liesack, Werner
Unexpected Dominance of Elusive Acidobacteria in Early Industrial Soft Coal Slags
title Unexpected Dominance of Elusive Acidobacteria in Early Industrial Soft Coal Slags
title_full Unexpected Dominance of Elusive Acidobacteria in Early Industrial Soft Coal Slags
title_fullStr Unexpected Dominance of Elusive Acidobacteria in Early Industrial Soft Coal Slags
title_full_unstemmed Unexpected Dominance of Elusive Acidobacteria in Early Industrial Soft Coal Slags
title_short Unexpected Dominance of Elusive Acidobacteria in Early Industrial Soft Coal Slags
title_sort unexpected dominance of elusive acidobacteria in early industrial soft coal slags
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462947/
https://www.ncbi.nlm.nih.gov/pubmed/28642744
http://dx.doi.org/10.3389/fmicb.2017.01023
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