Genetic Variability of Koi Herpesvirus In vitro—A Natural Event?

Worldwide koi herpesvirus (KHV) causes high mortalities in Cyprinus carpio L. aquaculture. So far, it is unknown how the different variants of KHV have developed and how they spread in the fish, but also in the environmental water bodies. Therefore, a phylogenetic method based on variable number of...

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Autores principales: Klafack, Sandro, Wang, Qing, Zeng, Weiwei, Wang, Yingying, Li, Yingying, Zheng, Shucheng, Kempter, Jolanta, Lee, Pei-Yu, Matras, Marek, Bergmann, Sven M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462989/
https://www.ncbi.nlm.nih.gov/pubmed/28642739
http://dx.doi.org/10.3389/fmicb.2017.00982
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author Klafack, Sandro
Wang, Qing
Zeng, Weiwei
Wang, Yingying
Li, Yingying
Zheng, Shucheng
Kempter, Jolanta
Lee, Pei-Yu
Matras, Marek
Bergmann, Sven M.
author_facet Klafack, Sandro
Wang, Qing
Zeng, Weiwei
Wang, Yingying
Li, Yingying
Zheng, Shucheng
Kempter, Jolanta
Lee, Pei-Yu
Matras, Marek
Bergmann, Sven M.
author_sort Klafack, Sandro
collection PubMed
description Worldwide koi herpesvirus (KHV) causes high mortalities in Cyprinus carpio L. aquaculture. So far, it is unknown how the different variants of KHV have developed and how they spread in the fish, but also in the environmental water bodies. Therefore, a phylogenetic method based on variable number of tandem repeats (VNTR) was improved to gain deeper insights into the phylogeny of KHV and its possible worldwide distribution. Moreover, a VNTR-3 qPCR was designed which allows fast virus typing. This study presents a useful method for molecular tracing of diverse KHV types, variants, and lineages.
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spelling pubmed-54629892017-06-22 Genetic Variability of Koi Herpesvirus In vitro—A Natural Event? Klafack, Sandro Wang, Qing Zeng, Weiwei Wang, Yingying Li, Yingying Zheng, Shucheng Kempter, Jolanta Lee, Pei-Yu Matras, Marek Bergmann, Sven M. Front Microbiol Microbiology Worldwide koi herpesvirus (KHV) causes high mortalities in Cyprinus carpio L. aquaculture. So far, it is unknown how the different variants of KHV have developed and how they spread in the fish, but also in the environmental water bodies. Therefore, a phylogenetic method based on variable number of tandem repeats (VNTR) was improved to gain deeper insights into the phylogeny of KHV and its possible worldwide distribution. Moreover, a VNTR-3 qPCR was designed which allows fast virus typing. This study presents a useful method for molecular tracing of diverse KHV types, variants, and lineages. Frontiers Media S.A. 2017-06-08 /pmc/articles/PMC5462989/ /pubmed/28642739 http://dx.doi.org/10.3389/fmicb.2017.00982 Text en Copyright © 2017 Klafack, Wang, Zeng, Wang, Li, Zheng, Kempter, Lee, Matras and Bergmann. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Klafack, Sandro
Wang, Qing
Zeng, Weiwei
Wang, Yingying
Li, Yingying
Zheng, Shucheng
Kempter, Jolanta
Lee, Pei-Yu
Matras, Marek
Bergmann, Sven M.
Genetic Variability of Koi Herpesvirus In vitro—A Natural Event?
title Genetic Variability of Koi Herpesvirus In vitro—A Natural Event?
title_full Genetic Variability of Koi Herpesvirus In vitro—A Natural Event?
title_fullStr Genetic Variability of Koi Herpesvirus In vitro—A Natural Event?
title_full_unstemmed Genetic Variability of Koi Herpesvirus In vitro—A Natural Event?
title_short Genetic Variability of Koi Herpesvirus In vitro—A Natural Event?
title_sort genetic variability of koi herpesvirus in vitro—a natural event?
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5462989/
https://www.ncbi.nlm.nih.gov/pubmed/28642739
http://dx.doi.org/10.3389/fmicb.2017.00982
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