Cargando…

Tim-3 Up-regulation in Patients with Gastric Cancer and Peptic Ulcer Disease

BACKGROUND: T-cell immunoglobulin and mucin domain protein-3 (Tim-3), an inhibitory immunoregulatory receptor, has been recently implicated in tumor biology and tumor-associated immune suppression. In the present study, expression of Tim-3 was evaluated in gastric cancer (GC) and peptic ulcer diseas...

Descripción completa

Detalles Bibliográficos
Autores principales: Naghavi-Alhosseini, Mahdieh, Tehrani, Mohsen, Ajami, Abolghasem, Rafiei, Alireza, Taghvaei, Tarang, Vahedi-Larijani, Laleh, Hossein-Nataj, Hadi, Asgarian-Omran, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5464497/
https://www.ncbi.nlm.nih.gov/pubmed/28441784
http://dx.doi.org/10.22034/APJCP.2017.18.3.765
_version_ 1783242781034020864
author Naghavi-Alhosseini, Mahdieh
Tehrani, Mohsen
Ajami, Abolghasem
Rafiei, Alireza
Taghvaei, Tarang
Vahedi-Larijani, Laleh
Hossein-Nataj, Hadi
Asgarian-Omran, Hossein
author_facet Naghavi-Alhosseini, Mahdieh
Tehrani, Mohsen
Ajami, Abolghasem
Rafiei, Alireza
Taghvaei, Tarang
Vahedi-Larijani, Laleh
Hossein-Nataj, Hadi
Asgarian-Omran, Hossein
author_sort Naghavi-Alhosseini, Mahdieh
collection PubMed
description BACKGROUND: T-cell immunoglobulin and mucin domain protein-3 (Tim-3), an inhibitory immunoregulatory receptor, has been recently implicated in tumor biology and tumor-associated immune suppression. In the present study, expression of Tim-3 was evaluated in gastric cancer (GC) and peptic ulcer disease (PUD) at both mRNA and protein levels. METHODS: A total of 133 gastric tissue biopsies, comprising 43 from GC cases, 48 from PUD and 42 from non-ulcer dyspepsia (NUD) serving as controls were collected. Additionally, non-neoplastic adjacent tissue biopsies were also obtained from 6 patients with GC. Infection with Helicobacter pylori was determined by the rapid urease test for all participants and H&E staining was conducted for GC and PUD patients. Tim-3 relative mRNA expression was determined by SYBR Green based Real-Time PCR using β-actin as a reference gene. Tim-3 protein expression was also studied by immunohistochemistry in 7 GC, 7 PUD and 10 NUD tissue samples. RESULTS: Tim-3 was expressed at higher levels in GC (p=0.030) and PUD (p=0.022) cases compared to he NUD group. Among paired samples obtained from gastric cancer patients, tumor tissues showed elevated Tim-3 expression (p=0.019) in comparison with adjacent non-neoplastic biopsies. Tim-3 mRNA findings were supported by detection of more Tim-3 protein in cancerous (p=0.002) and ulcerative (p=0.01) tissues than in controls. Tim-3 was similarly expressed in H. pylori positive and negative cases. CONCLUSION: Higher Tim-3 expression in patients with gastric cancer and peptic ulcer implies that it might be involved in immune regulation and establishment of these gastrointestinal diseases. Targeted immunotherapy by blocking of inhibitory receptors like Tim-3 could be a promising approach for gastric cancer treatment.
format Online
Article
Text
id pubmed-5464497
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher West Asia Organization for Cancer Prevention
record_format MEDLINE/PubMed
spelling pubmed-54644972017-08-28 Tim-3 Up-regulation in Patients with Gastric Cancer and Peptic Ulcer Disease Naghavi-Alhosseini, Mahdieh Tehrani, Mohsen Ajami, Abolghasem Rafiei, Alireza Taghvaei, Tarang Vahedi-Larijani, Laleh Hossein-Nataj, Hadi Asgarian-Omran, Hossein Asian Pac J Cancer Prev Research Article BACKGROUND: T-cell immunoglobulin and mucin domain protein-3 (Tim-3), an inhibitory immunoregulatory receptor, has been recently implicated in tumor biology and tumor-associated immune suppression. In the present study, expression of Tim-3 was evaluated in gastric cancer (GC) and peptic ulcer disease (PUD) at both mRNA and protein levels. METHODS: A total of 133 gastric tissue biopsies, comprising 43 from GC cases, 48 from PUD and 42 from non-ulcer dyspepsia (NUD) serving as controls were collected. Additionally, non-neoplastic adjacent tissue biopsies were also obtained from 6 patients with GC. Infection with Helicobacter pylori was determined by the rapid urease test for all participants and H&E staining was conducted for GC and PUD patients. Tim-3 relative mRNA expression was determined by SYBR Green based Real-Time PCR using β-actin as a reference gene. Tim-3 protein expression was also studied by immunohistochemistry in 7 GC, 7 PUD and 10 NUD tissue samples. RESULTS: Tim-3 was expressed at higher levels in GC (p=0.030) and PUD (p=0.022) cases compared to he NUD group. Among paired samples obtained from gastric cancer patients, tumor tissues showed elevated Tim-3 expression (p=0.019) in comparison with adjacent non-neoplastic biopsies. Tim-3 mRNA findings were supported by detection of more Tim-3 protein in cancerous (p=0.002) and ulcerative (p=0.01) tissues than in controls. Tim-3 was similarly expressed in H. pylori positive and negative cases. CONCLUSION: Higher Tim-3 expression in patients with gastric cancer and peptic ulcer implies that it might be involved in immune regulation and establishment of these gastrointestinal diseases. Targeted immunotherapy by blocking of inhibitory receptors like Tim-3 could be a promising approach for gastric cancer treatment. West Asia Organization for Cancer Prevention 2017 /pmc/articles/PMC5464497/ /pubmed/28441784 http://dx.doi.org/10.22034/APJCP.2017.18.3.765 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Research Article
Naghavi-Alhosseini, Mahdieh
Tehrani, Mohsen
Ajami, Abolghasem
Rafiei, Alireza
Taghvaei, Tarang
Vahedi-Larijani, Laleh
Hossein-Nataj, Hadi
Asgarian-Omran, Hossein
Tim-3 Up-regulation in Patients with Gastric Cancer and Peptic Ulcer Disease
title Tim-3 Up-regulation in Patients with Gastric Cancer and Peptic Ulcer Disease
title_full Tim-3 Up-regulation in Patients with Gastric Cancer and Peptic Ulcer Disease
title_fullStr Tim-3 Up-regulation in Patients with Gastric Cancer and Peptic Ulcer Disease
title_full_unstemmed Tim-3 Up-regulation in Patients with Gastric Cancer and Peptic Ulcer Disease
title_short Tim-3 Up-regulation in Patients with Gastric Cancer and Peptic Ulcer Disease
title_sort tim-3 up-regulation in patients with gastric cancer and peptic ulcer disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5464497/
https://www.ncbi.nlm.nih.gov/pubmed/28441784
http://dx.doi.org/10.22034/APJCP.2017.18.3.765
work_keys_str_mv AT naghavialhosseinimahdieh tim3upregulationinpatientswithgastriccancerandpepticulcerdisease
AT tehranimohsen tim3upregulationinpatientswithgastriccancerandpepticulcerdisease
AT ajamiabolghasem tim3upregulationinpatientswithgastriccancerandpepticulcerdisease
AT rafieialireza tim3upregulationinpatientswithgastriccancerandpepticulcerdisease
AT taghvaeitarang tim3upregulationinpatientswithgastriccancerandpepticulcerdisease
AT vahedilarijanilaleh tim3upregulationinpatientswithgastriccancerandpepticulcerdisease
AT hosseinnatajhadi tim3upregulationinpatientswithgastriccancerandpepticulcerdisease
AT asgarianomranhossein tim3upregulationinpatientswithgastriccancerandpepticulcerdisease