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Comparing Apoptosis and Necrosis Effects of Arctium Lappa Root Extract and Doxorubicin on MCF7 and MDA-MB-231 Cell Lines

OBJECTIVE: Breast cancer is a heterogeneous disease and very common malignancy in women worldwide. The efficacy of chemotherapy as an important part of breast cancer treatment is limited due to its side effects. While pharmaceutical companies are looking for better chemicals, research on traditional...

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Autores principales: Ghafari, Fereshteh, Rajabi, Mohammad Reza, Mazoochi, Tahereh, Taghizadeh, Mohsen, Nikzad, Hossein, Atlasi, Mohammad Ali, Taherian, Aliakbar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5464502/
https://www.ncbi.nlm.nih.gov/pubmed/28441789
http://dx.doi.org/10.22034/APJCP.2017.18.3.795
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author Ghafari, Fereshteh
Rajabi, Mohammad Reza
Mazoochi, Tahereh
Taghizadeh, Mohsen
Nikzad, Hossein
Atlasi, Mohammad Ali
Taherian, Aliakbar
author_facet Ghafari, Fereshteh
Rajabi, Mohammad Reza
Mazoochi, Tahereh
Taghizadeh, Mohsen
Nikzad, Hossein
Atlasi, Mohammad Ali
Taherian, Aliakbar
author_sort Ghafari, Fereshteh
collection PubMed
description OBJECTIVE: Breast cancer is a heterogeneous disease and very common malignancy in women worldwide. The efficacy of chemotherapy as an important part of breast cancer treatment is limited due to its side effects. While pharmaceutical companies are looking for better chemicals, research on traditional medicines that generally have fewer side effects is quite interesting. In this study, apoptosis and necrosis effect of Arctium lappa and doxorubicin was compared in MCF7, and MDA-MB-231 cell lines. MATERIALS AND METHODS: MCF7 and MDA-MB-231 cells were cultured in RPMI 1640 containing 10% FBS and 100 U/ml penicillin/streptomycin. MTT assay and an annexin V/propidium iodide (AV/PI) kit were used respectively to compare the survival rate and apoptotic effects of different concentrations of doxorubicin and Arctium lappa root extract on MDA-MB-231 and MCF7 cells. RESULTS: Arctium lappa root extract was able to reduce cell viability of the two cell lines in a dose and time dependent manner similar to doxorubicin. Flow cytometry results showed that similar to doxorubicin, Arctium Lappa root extract had a dose and time dependent apoptosis effect on both cell lines. 10µg/mL of Arctium lappa root extract and 5 µM of doxorubicin showed the highest anti-proliferative and apoptosis effect in MCF7 and MDA231 cells. CONCLUSION: The MCF7 (ER/PR-) and MDA-MB-231 (ER/PR+) cell lines represent two major breast cancer subtypes. The similar anti-proliferative and apoptotic effects of Arctium lappa root extract and doxorubicin (which is a conventional chemotherapy drug) on two different breast cancer cell lines strongly suggests its anticancer effects and further studies.
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spelling pubmed-54645022017-08-28 Comparing Apoptosis and Necrosis Effects of Arctium Lappa Root Extract and Doxorubicin on MCF7 and MDA-MB-231 Cell Lines Ghafari, Fereshteh Rajabi, Mohammad Reza Mazoochi, Tahereh Taghizadeh, Mohsen Nikzad, Hossein Atlasi, Mohammad Ali Taherian, Aliakbar Asian Pac J Cancer Prev Research Article OBJECTIVE: Breast cancer is a heterogeneous disease and very common malignancy in women worldwide. The efficacy of chemotherapy as an important part of breast cancer treatment is limited due to its side effects. While pharmaceutical companies are looking for better chemicals, research on traditional medicines that generally have fewer side effects is quite interesting. In this study, apoptosis and necrosis effect of Arctium lappa and doxorubicin was compared in MCF7, and MDA-MB-231 cell lines. MATERIALS AND METHODS: MCF7 and MDA-MB-231 cells were cultured in RPMI 1640 containing 10% FBS and 100 U/ml penicillin/streptomycin. MTT assay and an annexin V/propidium iodide (AV/PI) kit were used respectively to compare the survival rate and apoptotic effects of different concentrations of doxorubicin and Arctium lappa root extract on MDA-MB-231 and MCF7 cells. RESULTS: Arctium lappa root extract was able to reduce cell viability of the two cell lines in a dose and time dependent manner similar to doxorubicin. Flow cytometry results showed that similar to doxorubicin, Arctium Lappa root extract had a dose and time dependent apoptosis effect on both cell lines. 10µg/mL of Arctium lappa root extract and 5 µM of doxorubicin showed the highest anti-proliferative and apoptosis effect in MCF7 and MDA231 cells. CONCLUSION: The MCF7 (ER/PR-) and MDA-MB-231 (ER/PR+) cell lines represent two major breast cancer subtypes. The similar anti-proliferative and apoptotic effects of Arctium lappa root extract and doxorubicin (which is a conventional chemotherapy drug) on two different breast cancer cell lines strongly suggests its anticancer effects and further studies. West Asia Organization for Cancer Prevention 2017 /pmc/articles/PMC5464502/ /pubmed/28441789 http://dx.doi.org/10.22034/APJCP.2017.18.3.795 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
spellingShingle Research Article
Ghafari, Fereshteh
Rajabi, Mohammad Reza
Mazoochi, Tahereh
Taghizadeh, Mohsen
Nikzad, Hossein
Atlasi, Mohammad Ali
Taherian, Aliakbar
Comparing Apoptosis and Necrosis Effects of Arctium Lappa Root Extract and Doxorubicin on MCF7 and MDA-MB-231 Cell Lines
title Comparing Apoptosis and Necrosis Effects of Arctium Lappa Root Extract and Doxorubicin on MCF7 and MDA-MB-231 Cell Lines
title_full Comparing Apoptosis and Necrosis Effects of Arctium Lappa Root Extract and Doxorubicin on MCF7 and MDA-MB-231 Cell Lines
title_fullStr Comparing Apoptosis and Necrosis Effects of Arctium Lappa Root Extract and Doxorubicin on MCF7 and MDA-MB-231 Cell Lines
title_full_unstemmed Comparing Apoptosis and Necrosis Effects of Arctium Lappa Root Extract and Doxorubicin on MCF7 and MDA-MB-231 Cell Lines
title_short Comparing Apoptosis and Necrosis Effects of Arctium Lappa Root Extract and Doxorubicin on MCF7 and MDA-MB-231 Cell Lines
title_sort comparing apoptosis and necrosis effects of arctium lappa root extract and doxorubicin on mcf7 and mda-mb-231 cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5464502/
https://www.ncbi.nlm.nih.gov/pubmed/28441789
http://dx.doi.org/10.22034/APJCP.2017.18.3.795
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