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Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity
T cells are mechanosensitive but the effect of stiffness on their functions is still debated. We characterize herein how human primary CD4(+) T cell functions are affected by stiffness within the physiological Young’s modulus range of 0.5 kPa to 100 kPa. Stiffness modulates T lymphocyte migration an...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5464771/ https://www.ncbi.nlm.nih.gov/pubmed/28594327 http://dx.doi.org/10.7554/eLife.23190 |
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author | Saitakis, Michael Dogniaux, Stéphanie Goudot, Christel Bufi, Nathalie Asnacios, Sophie Maurin, Mathieu Randriamampita, Clotilde Asnacios, Atef Hivroz, Claire |
author_facet | Saitakis, Michael Dogniaux, Stéphanie Goudot, Christel Bufi, Nathalie Asnacios, Sophie Maurin, Mathieu Randriamampita, Clotilde Asnacios, Atef Hivroz, Claire |
author_sort | Saitakis, Michael |
collection | PubMed |
description | T cells are mechanosensitive but the effect of stiffness on their functions is still debated. We characterize herein how human primary CD4(+) T cell functions are affected by stiffness within the physiological Young’s modulus range of 0.5 kPa to 100 kPa. Stiffness modulates T lymphocyte migration and morphological changes induced by TCR/CD3 triggering. Stiffness also increases TCR-induced immune system, metabolism and cell-cycle-related genes. Yet, upon TCR/CD3 stimulation, while cytokine production increases within a wide range of stiffness, from hundreds of Pa to hundreds of kPa, T cell metabolic properties and cell cycle progression are only increased by the highest stiffness tested (100 kPa). Finally, mechanical properties of adherent antigen-presenting cells modulate cytokine production by T cells. Together, these results reveal that T cells discriminate between the wide range of stiffness values found in the body and adapt their responses accordingly. DOI: http://dx.doi.org/10.7554/eLife.23190.001 |
format | Online Article Text |
id | pubmed-5464771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-54647712017-06-09 Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity Saitakis, Michael Dogniaux, Stéphanie Goudot, Christel Bufi, Nathalie Asnacios, Sophie Maurin, Mathieu Randriamampita, Clotilde Asnacios, Atef Hivroz, Claire eLife Biophysics and Structural Biology T cells are mechanosensitive but the effect of stiffness on their functions is still debated. We characterize herein how human primary CD4(+) T cell functions are affected by stiffness within the physiological Young’s modulus range of 0.5 kPa to 100 kPa. Stiffness modulates T lymphocyte migration and morphological changes induced by TCR/CD3 triggering. Stiffness also increases TCR-induced immune system, metabolism and cell-cycle-related genes. Yet, upon TCR/CD3 stimulation, while cytokine production increases within a wide range of stiffness, from hundreds of Pa to hundreds of kPa, T cell metabolic properties and cell cycle progression are only increased by the highest stiffness tested (100 kPa). Finally, mechanical properties of adherent antigen-presenting cells modulate cytokine production by T cells. Together, these results reveal that T cells discriminate between the wide range of stiffness values found in the body and adapt their responses accordingly. DOI: http://dx.doi.org/10.7554/eLife.23190.001 eLife Sciences Publications, Ltd 2017-06-08 /pmc/articles/PMC5464771/ /pubmed/28594327 http://dx.doi.org/10.7554/eLife.23190 Text en © 2017, Saitakis et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biophysics and Structural Biology Saitakis, Michael Dogniaux, Stéphanie Goudot, Christel Bufi, Nathalie Asnacios, Sophie Maurin, Mathieu Randriamampita, Clotilde Asnacios, Atef Hivroz, Claire Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity |
title | Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity |
title_full | Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity |
title_fullStr | Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity |
title_full_unstemmed | Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity |
title_short | Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity |
title_sort | different tcr-induced t lymphocyte responses are potentiated by stiffness with variable sensitivity |
topic | Biophysics and Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5464771/ https://www.ncbi.nlm.nih.gov/pubmed/28594327 http://dx.doi.org/10.7554/eLife.23190 |
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