Cargando…
Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices
Neutrophil trafficking in tissues critically regulates the body’s immune response. Neutrophil migration can either play a protective role in host defense or cause health problems. Fibroblast growth factor 23 (FGF23) is a known biomarker for chronic kidney disease (CKD) and was recently shown to impa...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465076/ https://www.ncbi.nlm.nih.gov/pubmed/28596573 http://dx.doi.org/10.1038/s41598-017-03210-0 |
_version_ | 1783242881334509568 |
---|---|
author | Yang, Ke Peretz-Soroka, Hagit Wu, Jiandong Zhu, Ling Cui, Xueling Zhang, Michael Rigatto, Claudio Liu, Yong Lin, Francis |
author_facet | Yang, Ke Peretz-Soroka, Hagit Wu, Jiandong Zhu, Ling Cui, Xueling Zhang, Michael Rigatto, Claudio Liu, Yong Lin, Francis |
author_sort | Yang, Ke |
collection | PubMed |
description | Neutrophil trafficking in tissues critically regulates the body’s immune response. Neutrophil migration can either play a protective role in host defense or cause health problems. Fibroblast growth factor 23 (FGF23) is a known biomarker for chronic kidney disease (CKD) and was recently shown to impair neutrophil arrest on endothelium and transendothelial migration. In the present study, we further examined the effect of FGF23 on human blood neutrophil chemotaxis using two new microfluidic devices. Our results showed that chemotaxis of FGF23 pre-treated neutrophils to a fMLP gradient, in the presence or absence of a uniform FGF23 background, is quantitatively lower compared to the control cells. This effect is accompanied with a stronger drifting of FGF23 pre-treated cells along the flow. However, without the FGF23 pre-treatment, the FGF23 background only reduces chemotaxis of transmigrated cells through the thin barrier channel to the fMLP gradient. The effect of FGF23 on neutrophil migration and the correlation between multiple cell migration parameters are further revealed by chemotactic entropy and principle component analysis. Collectively, these results revealed the effect of FGF23 on weakening neutrophil chemotaxis, which shed light on FGF23 mediated neutrophil migration with direct disease relevance such as CKD. |
format | Online Article Text |
id | pubmed-5465076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54650762017-06-14 Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices Yang, Ke Peretz-Soroka, Hagit Wu, Jiandong Zhu, Ling Cui, Xueling Zhang, Michael Rigatto, Claudio Liu, Yong Lin, Francis Sci Rep Article Neutrophil trafficking in tissues critically regulates the body’s immune response. Neutrophil migration can either play a protective role in host defense or cause health problems. Fibroblast growth factor 23 (FGF23) is a known biomarker for chronic kidney disease (CKD) and was recently shown to impair neutrophil arrest on endothelium and transendothelial migration. In the present study, we further examined the effect of FGF23 on human blood neutrophil chemotaxis using two new microfluidic devices. Our results showed that chemotaxis of FGF23 pre-treated neutrophils to a fMLP gradient, in the presence or absence of a uniform FGF23 background, is quantitatively lower compared to the control cells. This effect is accompanied with a stronger drifting of FGF23 pre-treated cells along the flow. However, without the FGF23 pre-treatment, the FGF23 background only reduces chemotaxis of transmigrated cells through the thin barrier channel to the fMLP gradient. The effect of FGF23 on neutrophil migration and the correlation between multiple cell migration parameters are further revealed by chemotactic entropy and principle component analysis. Collectively, these results revealed the effect of FGF23 on weakening neutrophil chemotaxis, which shed light on FGF23 mediated neutrophil migration with direct disease relevance such as CKD. Nature Publishing Group UK 2017-06-08 /pmc/articles/PMC5465076/ /pubmed/28596573 http://dx.doi.org/10.1038/s41598-017-03210-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Yang, Ke Peretz-Soroka, Hagit Wu, Jiandong Zhu, Ling Cui, Xueling Zhang, Michael Rigatto, Claudio Liu, Yong Lin, Francis Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices |
title | Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices |
title_full | Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices |
title_fullStr | Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices |
title_full_unstemmed | Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices |
title_short | Fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices |
title_sort | fibroblast growth factor 23 weakens chemotaxis of human blood neutrophils in microfluidic devices |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465076/ https://www.ncbi.nlm.nih.gov/pubmed/28596573 http://dx.doi.org/10.1038/s41598-017-03210-0 |
work_keys_str_mv | AT yangke fibroblastgrowthfactor23weakenschemotaxisofhumanbloodneutrophilsinmicrofluidicdevices AT peretzsorokahagit fibroblastgrowthfactor23weakenschemotaxisofhumanbloodneutrophilsinmicrofluidicdevices AT wujiandong fibroblastgrowthfactor23weakenschemotaxisofhumanbloodneutrophilsinmicrofluidicdevices AT zhuling fibroblastgrowthfactor23weakenschemotaxisofhumanbloodneutrophilsinmicrofluidicdevices AT cuixueling fibroblastgrowthfactor23weakenschemotaxisofhumanbloodneutrophilsinmicrofluidicdevices AT zhangmichael fibroblastgrowthfactor23weakenschemotaxisofhumanbloodneutrophilsinmicrofluidicdevices AT rigattoclaudio fibroblastgrowthfactor23weakenschemotaxisofhumanbloodneutrophilsinmicrofluidicdevices AT liuyong fibroblastgrowthfactor23weakenschemotaxisofhumanbloodneutrophilsinmicrofluidicdevices AT linfrancis fibroblastgrowthfactor23weakenschemotaxisofhumanbloodneutrophilsinmicrofluidicdevices |