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Sortase A-mediated crosslinked short-chain dehydrogenases/reductases as novel biocatalysts with improved thermostability and catalytic efficiency
(S)-carbonyl reductase II (SCRII) from Candida parapsilosis is a short-chain alcohol dehydrogenase/reductase. It catalyses the conversion of 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol with low efficiency. Sortase was reported as a molecular “stapler” for site-specific protein conjugation t...
Autores principales: | Li, Kunpeng, Zhang, Rongzhen, Xu, Yan, Wu, Zhimeng, Li, Jing, Zhou, Xiaotian, Jiang, Jiawei, Liu, Haiyan, Xiao, Rong |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465079/ https://www.ncbi.nlm.nih.gov/pubmed/28596548 http://dx.doi.org/10.1038/s41598-017-03168-z |
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