Cargando…

Optimization of a CRISPR/Cas9-mediated Knock-in Strategy at the Porcine Rosa26 Locus in Porcine Foetal Fibroblasts

Genetically modified pigs have important roles in agriculture and biomedicine. However, genome-specific knock-in techniques in pigs are still in their infancy and optimal strategies have not been extensively investigated. In this study, we performed electroporation to introduce a targeting donor vec...

Descripción completa

Detalles Bibliográficos
Autores principales:	Xie, Zicong, Pang, Daxin, Wang, Kankan, Li, Mengjing, Guo, Nannan, Yuan, Hongming, Li, Jianing, Zou, Xiaodong, Jiao, Huping, Ouyang, Hongsheng, Li, Zhanjun, Tang, Xiaochun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465212/ https://www.ncbi.nlm.nih.gov/pubmed/28596588 http://dx.doi.org/10.1038/s41598-017-02785-y

Ejemplares similares

Site-Specific Fat-1 Knock-In Enables Significant Decrease of n-6PUFAs/n-3PUFAs Ratio in Pigs
por: Li, Mengjing, et al.
Publicado: (2018)

CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster
por: Lu, Chao, et al.
Publicado: (2019)

Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-directed Repair
por: Wang, Kankan, et al.
Publicado: (2016)

Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System
por: Wang, Kankan, et al.
Publicado: (2015)

CRISPR/Cas9-Mediated Specific Integration of Fat-1 and IGF-1 at the pRosa26 Locus
por: You, Wenni, et al.
Publicado: (2021)

CRISPR/Cas9-mediated knock-in strategy at the Rosa26 locus in cattle fetal fibroblasts
por: Xie, Yuxuan, et al.
Publicado: (2022)

HMEJ-mediated site-specific integration of a myostatin inhibitor increases skeletal muscle mass in porcine
por: Li, Mengjing, et al.
Publicado: (2021)

HMEJ-mediated site-specific integration of a myostatin inhibitor increases skeletal muscle mass in porcine
por: Li, Mengjing, et al.
Publicado: (2023)

Genetically modified pigs are protected from classical swine fever virus
por: Xie, Zicong, et al.
Publicado: (2018)

Large-Scale CRISPR Screen of LDLR Pathogenic Variants
por: Li, Mengjing, et al.
Publicado: (2023)

Rosa26-targeted sheep gene knock-in via CRISPR-Cas9 system
por: Wu, Mingming, et al.
Publicado: (2016)

Pathological alterations in the gastrointestinal tract of a porcine model of DMD
por: Zou, Xiaodong, et al.
Publicado: (2021)

Porcine Epidemic Diarrhea Virus: An Updated Overview of Virus Epidemiology, Virulence Variation Patterns and Virus–Host Interactions
por: Zhang, Yuanzhu, et al.
Publicado: (2022)

Efficiency of porcine somatic cell nuclear transfer – a retrospective study of factors related to embryo recipient and embryos transferred
por: Huang, Yongye, et al.
Publicado: (2013)

Efficient targeted integration into the bovine Rosa26 locus using TALENs
por: Wang, Ming, et al.
Publicado: (2018)

Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes
por: Park, Ki-Eun, et al.
Publicado: (2017)

High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi
por: Hohenstein, Peter, et al.
Publicado: (2008)

Identification and characterization of rabbit ROSA26 for gene knock-in and stable reporter gene expression
por: Yang, Dongshan, et al.
Publicado: (2016)

Porcine Knock-in Fibroblasts Expressing hDAF on α-1,3-Galactosyltransferase (GGTA1) Gene Locus
por: Kim, Ji Woo, et al.
Publicado: (2012)

Long-Term Evaluation of Retinal Morphology and Function in Rosa26-Cas9 Knock-In Mice
por: Mohan, Kabhilan, et al.
Publicado: (2023)

Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes
por: Chu, Van Trung, et al.
Publicado: (2016)

Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins
por: Ouyang, Ting, et al.
Publicado: (2019)

Dual Fluorescent Reporter Pig for Cre Recombination: Transgene Placement at the ROSA26 Locus
por: Li, Shun, et al.
Publicado: (2014)

Expression of Transgenes Targeted to the Gt(ROSA)26Sor Locus Is Orientation Dependent
por: Strathdee, Douglas, et al.
Publicado: (2006)

Gene targeting to the ROSA26 locus directed by engineered zinc finger nucleases
por: Perez-Pinera, Pablo, et al.
Publicado: (2012)

TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis
por: Wang, Yanbing, et al.
Publicado: (2022)

Correction: Wang et al. TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis. Biology 2022, 11, 141
por: Wang, Yanbing, et al.
Publicado: (2022)

Association of foetal size and sex with porcine foeto-maternal interface integrin expression
por: Stenhouse, Claire, et al.
Publicado: (2019)

Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
por: Srinivas, Shankar, et al.
Publicado: (2001)

Genomic Imprinting at the Porcine DIRAS3 Locus
por: Ahn, Jinsoo, et al.
Publicado: (2021)

Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system
por: Quadros, Rolen M., et al.
Publicado: (2015)

Highly Efficient Transgenesis in Ferrets Using CRISPR/Cas9-Mediated Homology-Independent Insertion at the ROSA26 Locus
por: Yu, Miao, et al.
Publicado: (2019)

Characteristics and neural-like differentiation of mesenchymal stem cells derived from foetal porcine bone marrow
por: Liu, Ying, et al.
Publicado: (2013)

Immunogenicity evaluation of inactivated virus and purified proteins of porcine circovirus type 2 in mice
por: Liu, Xiaohui, et al.
Publicado: (2018)

Genomic Imprinting at the Porcine PLAGL1 Locus and the Orthologous Locus in the Human
por: Ahn, Jinsoo, et al.
Publicado: (2021)

A Modified RMCE-Compatible Rosa26 Locus for the Expression of Transgenes from Exogenous Promoters
por: Tchorz, Jan S., et al.
Publicado: (2012)

Rosa26 Locus Supports Tissue-Specific Promoter Driving Transgene Expression Specifically in Pig
por: Kong, Qingran, et al.
Publicado: (2014)

Feasibility Study of a Novel Transcatheter Tricuspid Annuloplasty System in a Porcine Model
por: Pan, Wenzhi, et al.
Publicado: (2022)

Evaluation of OPEN Zinc Finger Nucleases for Direct Gene Targeting of the ROSA26 Locus in Mouse Embryos
por: Hermann, Mario, et al.
Publicado: (2012)

Transvection-like interchromosomal interaction is not observed at the transcriptional level when tested in the Rosa26 locus in mouse
por: Tanimoto, Keiji, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_jgq3vkab755p0mp71m6pn3r0uf