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Live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared II fluorophore

In vivo imaging of hormone receptors provides the opportunity to visualize target tissues under hormonal control in live animals. Detecting longer-wavelength photons in the second near-infrared window (NIR-II, 1000–1700 nm) region affords reduced photon scattering in tissues accompanied by lower aut...

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Autores principales: Feng, Yi, Zhu, Shoujun, Antaris, Alexander L., Chen, Hao, Xiao, Yuling, Lu, Xiaowei, Jiang, Linlin, Diao, Shuo, Yu, Kuai, Wang, Yan, Herraiz, Sonia, Yue, Jingying, Hong, Xuechuan, Hong, Guosong, Cheng, Zhen, Dai, Hongjie, Hsueh, Aaron J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465568/
https://www.ncbi.nlm.nih.gov/pubmed/28626555
http://dx.doi.org/10.1039/c6sc04897h
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author Feng, Yi
Zhu, Shoujun
Antaris, Alexander L.
Chen, Hao
Xiao, Yuling
Lu, Xiaowei
Jiang, Linlin
Diao, Shuo
Yu, Kuai
Wang, Yan
Herraiz, Sonia
Yue, Jingying
Hong, Xuechuan
Hong, Guosong
Cheng, Zhen
Dai, Hongjie
Hsueh, Aaron J.
author_facet Feng, Yi
Zhu, Shoujun
Antaris, Alexander L.
Chen, Hao
Xiao, Yuling
Lu, Xiaowei
Jiang, Linlin
Diao, Shuo
Yu, Kuai
Wang, Yan
Herraiz, Sonia
Yue, Jingying
Hong, Xuechuan
Hong, Guosong
Cheng, Zhen
Dai, Hongjie
Hsueh, Aaron J.
author_sort Feng, Yi
collection PubMed
description In vivo imaging of hormone receptors provides the opportunity to visualize target tissues under hormonal control in live animals. Detecting longer-wavelength photons in the second near-infrared window (NIR-II, 1000–1700 nm) region affords reduced photon scattering in tissues accompanied by lower autofluorescence, leading to higher spatial resolution at up to centimeter tissue penetration depths. Here, we report the conjugation of a small molecular NIR-II fluorophore CH1055 to a follicle stimulating hormone (FSH-CH) for imaging ovaries and testes in live mice. After exposure to FSH-CH, specific NIR-II signals were found in cultured ovarian granulosa cells containing FSH receptors. Injection of FSH-CH allowed live imaging of ovarian follicles and testicular seminiferous tubules in female and male adult mice, respectively. Using prepubertal mice, NIR-II signals were detected in ovaries containing only preantral follicles. Resolving earlier controversies regarding the expression of FSH receptors in cultured osteoclasts, we detected for the first time specific FSH receptor signals in bones in vivo. The present imaging of FSH receptors in live animals using a ligand-conjugated NIR-II fluorophore with low cell toxicity and rapid clearance allows the development of non-invasive molecular imaging of diverse hormonal target cells in vivo.
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spelling pubmed-54655682017-06-16 Live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared II fluorophore Feng, Yi Zhu, Shoujun Antaris, Alexander L. Chen, Hao Xiao, Yuling Lu, Xiaowei Jiang, Linlin Diao, Shuo Yu, Kuai Wang, Yan Herraiz, Sonia Yue, Jingying Hong, Xuechuan Hong, Guosong Cheng, Zhen Dai, Hongjie Hsueh, Aaron J. Chem Sci Chemistry In vivo imaging of hormone receptors provides the opportunity to visualize target tissues under hormonal control in live animals. Detecting longer-wavelength photons in the second near-infrared window (NIR-II, 1000–1700 nm) region affords reduced photon scattering in tissues accompanied by lower autofluorescence, leading to higher spatial resolution at up to centimeter tissue penetration depths. Here, we report the conjugation of a small molecular NIR-II fluorophore CH1055 to a follicle stimulating hormone (FSH-CH) for imaging ovaries and testes in live mice. After exposure to FSH-CH, specific NIR-II signals were found in cultured ovarian granulosa cells containing FSH receptors. Injection of FSH-CH allowed live imaging of ovarian follicles and testicular seminiferous tubules in female and male adult mice, respectively. Using prepubertal mice, NIR-II signals were detected in ovaries containing only preantral follicles. Resolving earlier controversies regarding the expression of FSH receptors in cultured osteoclasts, we detected for the first time specific FSH receptor signals in bones in vivo. The present imaging of FSH receptors in live animals using a ligand-conjugated NIR-II fluorophore with low cell toxicity and rapid clearance allows the development of non-invasive molecular imaging of diverse hormonal target cells in vivo. Royal Society of Chemistry 2017-05-01 2017-03-06 /pmc/articles/PMC5465568/ /pubmed/28626555 http://dx.doi.org/10.1039/c6sc04897h Text en This journal is © The Royal Society of Chemistry 2017 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Feng, Yi
Zhu, Shoujun
Antaris, Alexander L.
Chen, Hao
Xiao, Yuling
Lu, Xiaowei
Jiang, Linlin
Diao, Shuo
Yu, Kuai
Wang, Yan
Herraiz, Sonia
Yue, Jingying
Hong, Xuechuan
Hong, Guosong
Cheng, Zhen
Dai, Hongjie
Hsueh, Aaron J.
Live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared II fluorophore
title Live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared II fluorophore
title_full Live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared II fluorophore
title_fullStr Live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared II fluorophore
title_full_unstemmed Live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared II fluorophore
title_short Live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared II fluorophore
title_sort live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared ii fluorophore
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465568/
https://www.ncbi.nlm.nih.gov/pubmed/28626555
http://dx.doi.org/10.1039/c6sc04897h
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