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Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal

AIM: The aim of this work was to detect antibiotic resistance in Gram-negative bacteria isolated from subclinical mastitis in cattle in West Bengal. MATERIALS AND METHODS: The milk samples were collected from the cattle suffering with subclinical mastitis in West Bengal. The milk samples were inocul...

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Autores principales: Das, Arnab, Guha, Chanchal, Biswas, Ujjwal, Jana, Partha Sarathi, Chatterjee, Amaresh, Samanta, Indranil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465765/
https://www.ncbi.nlm.nih.gov/pubmed/28620255
http://dx.doi.org/10.14202/vetworld.2017.517-520
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author Das, Arnab
Guha, Chanchal
Biswas, Ujjwal
Jana, Partha Sarathi
Chatterjee, Amaresh
Samanta, Indranil
author_facet Das, Arnab
Guha, Chanchal
Biswas, Ujjwal
Jana, Partha Sarathi
Chatterjee, Amaresh
Samanta, Indranil
author_sort Das, Arnab
collection PubMed
description AIM: The aim of this work was to detect antibiotic resistance in Gram-negative bacteria isolated from subclinical mastitis in cattle in West Bengal. MATERIALS AND METHODS: The milk samples were collected from the cattle suffering with subclinical mastitis in West Bengal. The milk samples were inoculated into the nutrient broth and incubated at 37°C. On the next day, the growth was transferred into nutrient agar and MacConkey agar. All the pure cultures obtained from nutrient agar slant were subjected to Gram-staining and standard biochemical tests. All the bacterial isolates were tested in vitro for their sensitivity to different antibiotics commonly used in veterinary practices. All Gram-negative isolates including positive control were subjected to polymerase chain reaction (PCR) for detection of bla(CTX-M), bla(TEM), bla(SHV), bla(VIM), tetA, tetB, tetC, and tetM genes considered for extended-spectrum β-lactamase (ESBL), metallo-β-lactamase, and tetracycline resistance. RESULTS: In total, 50 Gram-negative organisms (Escherichia coli, Proteus, Pseudomonas, Klebsiella, and Enterobacter) were isolated from milk samples of subclinical mastitis infected cattle. Among these Gram-negative isolates, 48% (24/50) were found either ESBL producing or tetracycline resistant. Out of total 50 Gram-negative isolates, bla(CTX-M) was detected in 18 (36%) isolates, and 6 (12%) harbored bla(TEM) genes in PCR. None of the isolates carried bla(SHV) genes. Further, in this study, 5 (10%) isolates harbored tet(A) gene, and 8 (16%) isolates carried tet(B) gene. No tet(C) gene was detected from the isolates. CONCLUSION: This study showed emerging trend of antibiotic-resistant Gram-negative bacteria associated with subclinical mastitis in cattle in West Bengal, India.
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spelling pubmed-54657652017-06-15 Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal Das, Arnab Guha, Chanchal Biswas, Ujjwal Jana, Partha Sarathi Chatterjee, Amaresh Samanta, Indranil Vet World Research Article AIM: The aim of this work was to detect antibiotic resistance in Gram-negative bacteria isolated from subclinical mastitis in cattle in West Bengal. MATERIALS AND METHODS: The milk samples were collected from the cattle suffering with subclinical mastitis in West Bengal. The milk samples were inoculated into the nutrient broth and incubated at 37°C. On the next day, the growth was transferred into nutrient agar and MacConkey agar. All the pure cultures obtained from nutrient agar slant were subjected to Gram-staining and standard biochemical tests. All the bacterial isolates were tested in vitro for their sensitivity to different antibiotics commonly used in veterinary practices. All Gram-negative isolates including positive control were subjected to polymerase chain reaction (PCR) for detection of bla(CTX-M), bla(TEM), bla(SHV), bla(VIM), tetA, tetB, tetC, and tetM genes considered for extended-spectrum β-lactamase (ESBL), metallo-β-lactamase, and tetracycline resistance. RESULTS: In total, 50 Gram-negative organisms (Escherichia coli, Proteus, Pseudomonas, Klebsiella, and Enterobacter) were isolated from milk samples of subclinical mastitis infected cattle. Among these Gram-negative isolates, 48% (24/50) were found either ESBL producing or tetracycline resistant. Out of total 50 Gram-negative isolates, bla(CTX-M) was detected in 18 (36%) isolates, and 6 (12%) harbored bla(TEM) genes in PCR. None of the isolates carried bla(SHV) genes. Further, in this study, 5 (10%) isolates harbored tet(A) gene, and 8 (16%) isolates carried tet(B) gene. No tet(C) gene was detected from the isolates. CONCLUSION: This study showed emerging trend of antibiotic-resistant Gram-negative bacteria associated with subclinical mastitis in cattle in West Bengal, India. Veterinary World 2017-05 2017-05-12 /pmc/articles/PMC5465765/ /pubmed/28620255 http://dx.doi.org/10.14202/vetworld.2017.517-520 Text en Copyright: © Das, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Das, Arnab
Guha, Chanchal
Biswas, Ujjwal
Jana, Partha Sarathi
Chatterjee, Amaresh
Samanta, Indranil
Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title_full Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title_fullStr Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title_full_unstemmed Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title_short Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title_sort detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in west bengal
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465765/
https://www.ncbi.nlm.nih.gov/pubmed/28620255
http://dx.doi.org/10.14202/vetworld.2017.517-520
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